[48] The use of alkaline phosphatase-conjugated anti-immunoglobulin with immunoblots for determining the specificity of monoclonal antibodies to protein mixtures

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Continuous cultures of fused cells secreting antibody of predefined specificity

1975 • 17,037 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

A rapid, sensitive, and specific method for the determination of protein in dilute solution

1973 • 2,518 citations

A dot-immunobinding assay for monoclonal and other antibodies

1982 • 2,219 citations

A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots

1984 • 2,189 citations

Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose

1984 • 1,697 citations

Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli K12 into four bands

1975 • 1,439 citations

Protein blotting: Principles and applications

1983 • 858 citations

Antibody production by hybridomas

1980 • 646 citations

The use of Coomassie Brilliant Blue G250 perchloric acid solution for staining in electrophoresis and isoelectric focusing on polyacrylamide gels

1975 • 622 citations

Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents.

1978 • 408 citations

Coupling of Enzymes to Antibodies and Antigens

1978 • 344 citations

A tetrazolium method for non-specific alkaline phosphatase

1970 • 274 citations

On the electrotransfer of polypeptides from gels to nitrocellulose membranes

1983 • 192 citations

Quantitative electrophoretic transfer of polypeptides from SDS polyacrylamide gels to nitrocellulose sheets: A method for their re-use in immunoautoradiographic detection of antigens

1982 • 190 citations

Heterogeneity of the ribosomal genes in mice and men

1977 • 138 citations

Application of the nitrocellulose transfer technique and alkaline phosphatase conjugated anti-immunoglobulin for determination of the specificity of monoclonal antibodies to protein mixtures

1982 • 113 citations

Western and dot immunoblotting analysis of viral antigens and antibodies: Application to murine hepatitis virus

1984 • 101 citations

The use of alkaline-phosphatase-conjugated second antibody for the visualization of electrophoretically separated proteins recognized by monoclonal antibodies

1983 • 67 citations

Calf thymus alkaline phosphatase

1977 • 32 citations

Immunoblotting with monoclonal antibodies: Loss of immunoreactivity with human immunoglobulins arises from polypeptide chain separation

1984 • 28 citations

Enumeration of lymphocyte populations in whole peripheral blood with alkaline phosphatase-labelled reagents. A method for routine clinical use.

1977 • 21 citations

One antigen may form two precipitin lines and two spurs when tested with two monoclonal antibodies by gel diffusion assays

1984 • 17 citations

Detection of anti-TNP antibody-forming cells (AFC) with TNP-enzyme and TNP-Fab anti-enzyme conjugates

1976 • 10 citations