Coupling of Enzymes to Antibodies and Antigens

Methods of enzymatic analysis

1964 • 5,230 citations

PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPY

1974 • 3,882 citations

Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G

1971 • 3,630 citations

PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATION

1974 • 2,413 citations

Coupling of enzymes to proteins with glutaraldehyde

1969 • 1,442 citations

A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. Coli I. Purification and characterization of alkaline phosphatase

1960 • 1,407 citations

Immunoassay using antibody—enzyme conjugates

1974 • 883 citations

Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration

1971 • 879 citations

The steady-state kinetics of peroxidase with 2,2′-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen

1975 • 871 citations

Purification, Composition, and Molecular Weight of the β-Galactosidase of Escherichia coli K12

1965 • 660 citations

ENZYME-LABELED ANTIBODIES FOR THE LIGHT AND ELECTRON MICROSCOPIC LOCALIZATION OF TISSUE ANTIGENS

1967 • 614 citations

ENZYME AND METABOLIC INHIBITORS

1964 • 504 citations

THE PROPERTIES OF SPECIFIC STAINS FOR ELECTRON MICROSCOPY PREPARED BY THE CONJUGATION OF ANTIBODY MOLECULES WITH FERRITIN

1961 • 266 citations

Enzyme-linked immunoassay: conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from E. coli and its use for immunoassay.

1976 • 153 citations

Immunoenzyme Techniques: Enzymes as Markers for the Localization of Antigens and Antibodies

1970 • 137 citations

FLUOROGENIC SUBSTRATES FOR β-D-GALACTOSIDASES AND PHOSPHATASES DERIVED FROM FLUORESCEIN (3, 6-DIHYDROXYFLUORAN) AND ITS MONOMETHYL ETHER

1963 • 128 citations

On the Formation of Covalent Linkages between Two Protein Molecules

1961 • 124 citations

Polymerization and Immobilization of Proteins Using Ethylchloroformate and Glutaraldehyde

1976 • 123 citations

Covalent attachment of proteins to polysaccharide carriers by means of benzoquinone

1975 • 122 citations

A sandwich method of enzymoimmunoassay. I. Application to rat and human alpha-fetoprotein

1975 • 120 citations

Magnetic solid phase enzyme-immunoassay

1977 • 118 citations

Enzyme-Linked Immunoassay: Conjugation of the Fab′ Fragment of Rabbit IgG with β-D-Galactosidase from E. Coli and its Use for Immunoassay

1976 • 109 citations

DETECTION OF PLASMA MEMBRANE CARBOHYDRATES WITH LECTIN PEROXIDASE CONJUGATES

1973 • 107 citations

Enzyme-immunoassay for the measurement of antigens using peroxidase conjugates

1972 • 101 citations

Identification of synaptic acetylcholine receptor sites in retina with peroxidase-labeled α-bungarotoxin

1977 • 99 citations

A method for quantitative determination of cellular immunoglobulins by enzyme‐labeled antibodies

1971 • 98 citations

A comparative study of horseradish peroxidase conjugates prepared with a one-step and a two-step method.

1975 • 84 citations

Enzyme‐Linked Immunoassay

1976 • 78 citations

Peroxidase-conjugate chromatography isolation of conjugates prepared with glutaraldehyde or periodate using polyacrylamide-agarose gel.

1976 • 76 citations

Immunoenzymatic techniques

1977 • 67 citations

Enzyme-linked Immunoassay<subtitle>I. Novel Method for Synthesis of the Insulin-β-D-galactosidase Conjugate and Its Applicability for Insulin Assay</subtitle>

1975 • 66 citations

Enzyme markers: their linkage with proteins and use in immuno-histochemistry

1972 • 60 citations

ANTIBODY ENZYME CONJUGATES THE PREPARATION OF INTERMOLECULAR CONJUGATES OF HORSERADISH PEROXIDASE AND ANTIBODY AND THEIR USE IN IMMUNOHISTOLOGY OF RENAL CORTEX

1973 • 51 citations

ULTRASTRUCTURAL LOCALIZATION OF INTRACELLULAR ANTIGEN USING ENZYME-LABELED ANTIBODY FRAGMENTS

1971 • 51 citations

Enzyme markers: their linkage with proteins and use in immuno-histochemistry

1973 • 50 citations

A new method using p-benzoquinone for coupling antigens and antibodies to marker substances.

1976 • 42 citations

Conjugation of p-Benzoquinone treated enzymes with antibodies and Fab fragments

1977 • 40 citations

Peroxidase and fluorescein isothiocyanate as antibody markers. A quantitative comparison of two peroxidase conjugates prepared with glutaraldehyde or periodate anda fluorescein conjugate.

1976 • 39 citations

Enzyme Immunoassay of Insulin by Fluorimetry of the Insulin-glucoamylase Complex

1973 • 36 citations

Conjugation of antibody to ferritin by means of p,p′-difluoro-m,m′-dinitrodiphenylsulphone

1962 • 32 citations

Studies on the conjugation of horseradish peroxidase to Fab fragments

1973 • 29 citations

An enzyme-immunoassay of antibody specific for adenosine using β-galactosidase

1974 • 26 citations

Use of tolylene diisocyanate for the preparation of a peroxidase-labelled antibody conjugate

1973 • 26 citations

The reaction of 4,4′-difluoro-3,3′-dinitro-diphenyl sulfone with γ-globulin and horseradish peroxidase

1971 • 25 citations

An Enzyme-Linked Procedure for the Detection and Estimation of Surface Receptors on Cells

1976 • 15 citations

Conjugation of enzymes to anti-poliovirus globulin: Effect of enzyme molecular weight on virus neutralization capacity

1974 • 14 citations

The preparation of ferritin-labelled antibodies and other protein-protein conjugates with bis-diazotized benzidine

1967 • 11 citations

Conjugation of lactic-dehydrogenase with proteins by the use of glutaraldehyde: detection of antigens in the immunocompetent cells by means of the conjugates.

1969 • 4 citations