Effects of polystyrene surface chemistry on the biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies

Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule

1984 • 3,580 citations

Fibronectin adsorption on hydrophilic and hydrophobic surfaces detected by antibody binding and analyzed during cell adhesion in serum-containing medium.

1982 • 613 citations

Cell attachment to thrombospondin: the role of ARG-GLY-ASP, calcium, and integrin receptors.

1988 • 481 citations

Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule

1981 • 444 citations

Identification of an alternatively spliced site in human plasma fibronectin that mediates cell type-specific adhesion.

1986 • 442 citations

A β ₃ Integrin Mutation Abolishes Ligand Binding and Alters Divalent Cation-Dependent Conformation

1990 • 418 citations

Adsorption characteristics of plasma fibronectin in relationship to biological activity

1981 • 335 citations

The adsorptive characteristics of proteins for polystyrene and their significance in solid-phase immunoassays

1980 • 307 citations

Human fibronectin contains distinct adhesion- and motility-promoting domains for metastatic melanoma cells.

1986 • 270 citations

Affinity chromatographic isolation of the melanoma adhesion receptor for the IIICS region of fibronectin and its identification as the integrin alpha 4 beta 1.

1990 • 244 citations

A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin

1989 • 205 citations

Role of serum vitronectin and fibronectin in adhesion of fibroblasts following seeding onto tissue culture polystyrene

1992 • 171 citations

Monoclonal antibody characterization of two distant sites required for function of the central cell-binding domain of fibronectin in cell adhesion, cell migration, and matrix assembly.

1991 • 156 citations

Polystyrene chemistry affects vitronectin activity: An explanation for cell attachment to tissue culture polystyrene but not to unmodified polystyrene

1993 • 140 citations

Competitive adsorption of vitronection with albumin, fibrinogen, and fibronectin on polymeric biomaterials

1991 • 114 citations

Conformational states of vitronectin: preferential expression of an antigenic epitope when vitronectin is covalently and noncovalently complexed with thrombin-antithrombin III or treated with urea

1988 • 89 citations

Heparin-binding properties of human serum spreading factor.

1985 • 83 citations

Effects of plasma protein adsorption on protein conformation and activity

1984 • 76 citations

Conformational states of vitronectin: preferential expression of an antigenic epitope when vitronectin is covalently and noncovalently complexed with thrombin-antithrombin III or treated with urea

1988 • 69 citations

Activation of Vitronectin (Serum Spreading Factor) Binding of Heparin by Denaturing Agents1

1985 • 69 citations

Adhesion and growth of cultured human endothelial cells on perfluorosulphonate: pole of vitronectin and fibponectin in cell attachment

1991 • 66 citations

Induction of fibroblast spreading by Mn2+: A possible role for unusual binding sites for divalent cations in receptors for proteins containing arg-gly-asp

1988 • 63 citations

Secondary structure of human plasma fibronectin: conformational change induced by calf alveolar heparan sulfates

1985 • 57 citations

Conformational changes of plasma fibronectin detected upon adsorption to solid substrates: a spin-label study

1989 • 51 citations

Immunoglobulin G and secretory fibronectin adsorption to silica

1987 • 47 citations

Fluorescence energy transfer detects changes in fibronectin structure upon surface binding

1989 • 43 citations

Adsorption of Fibronectin to Polyurethane Surfaces: Fourier Transform Infrared Spectroscopic Studies

1987 • 42 citations

Vitronectin adsorption on polystyrene and oxidized polystyrene

1989 • 42 citations

Use of protein A to remove immunoglobulins from serum in hybridoma culture media

1983 • 40 citations

Structural changes in the NH2-terminal domain of fibronectin upon interaction with heparin. Relationship to matrix-driven translocation.

1988 • 38 citations

Practical limitations of estimation of protein adsorption to polymer surfaces

1991 • 30 citations

A major difference between serum and fibronectin in the divalent cation requirement for adhesion and spreading of BHK21 cells

1987 • 29 citations

Anti-fibronectin antibodies that modify heparin binding and cell adhesion: evidence for a new cell binding site in the heparin binding region

1992 • 27 citations

Heparin modulates conformational states of plasma fibronectin: An electron spin resonance spin label approach

1986 • 26 citations

Solid-phase monoclonal antibodies A novel method of directing the function of biologically active molecules by presenting a specific orientation

1990 • 22 citations

Practical considerations of the ability of monoclonal antibodies to detect antigenic differences between closely related variants

1985 • 21 citations

One free sulfhydryl group of plasma fibronectin becomes titratable upon binding of the protein to solid substrates

1988 • 16 citations

Use of Monoclonal Antibodies in the Study of the Conformation and Conformational Alterations in Proteins

1988 • 15 citations

Discrimination of Different Domains in Fibronectin on the Basis of their Stability against Urea Denaturation

1983 • 11 citations

A monoclonal antibody against brain calmodulin-dependent protein kinase type II detects putative conformational changes induced by calcium-calmodulin

1988 • 7 citations