Use of DNA sequence and mutant analyses and antisense oligodeoxynucleotides to examine the molecular basis of nonmuscle myosin light chain kinase autoinhibition, calmodulin recognition, and activity.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

The Determination of Enzyme Dissociation Constants

1934 • 11,865 citations

Improved tools for biological sequence comparison.

1988 • 11,455 citations

The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Catalytic Domains

1988 • 5,115 citations

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

1984 • 4,231 citations

The helical hydrophobic moment: a measure of the amphiphilicity of a helix

1982 • 1,030 citations

An oligomer complementary to c-myc mRNA inhibits proliferation of HL-60 promyelocytic cells and induces differentiation.

1988 • 476 citations

Inhibition of Cellular Proliferation by Antisense Oligodeoxynucleotides to PCNA Cyclin

1988 • 425 citations

Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for activity.

1979 • 381 citations

Sequence of an unusually large protein implicated in regulation of myosin activity in C. elegans

1989 • 368 citations

Identification of major nucleolar proteins as candidate mitotic substrates of cdc2 kinase

1990 • 344 citations

Loosening tight junctions. Lessons from the intestine.

1989 • 329 citations

Calmodulin binding domains: characterization of a phosphorylation and calmodulin binding site from myosin light chain kinase

1986 • 319 citations

Cloning and expression of a cDNA coding for the human platelet-derived growth factor receptor: evidence for more than one receptor class.

1988 • 314 citations

Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase.

1987 • 287 citations

Regulation of actin microfilament integrity in living nonmuscle cells by the cAMP-dependent protein kinase and the myosin light chain kinase.

1988 • 279 citations

Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning.

1987 • 253 citations

Involvement of myosin light-chain kinase in endothelial cell retraction.

1990 • 249 citations

The calmodulin binding domain of chicken smooth muscle myosin light chain kinase contains a pseudosubstrate sequence.

1987 • 241 citations

[30] Smooth muscle myosin light chain kinase

1983 • 229 citations

Molecular cloning of cDNA for caltractin, a basal body-associated Ca2+-binding protein: homology in its protein sequence with calmodulin and the yeast CDC31 gene product.

1988 • 219 citations

Regulatory and structural motifs of chicken gizzard myosin light chain kinase.

1990 • 208 citations

The Nature of the Intermolecular Forces Operative in Biological Processes

1940 • 205 citations

Homology of the .gamma. subunit of phosphorylase b kinase with cAMP-dependent protein kinase

1984 • 173 citations

Autoregulation of Enzymes by Pseudosubstrate Prototopes: Myosin Light Chain Kinase

1988 • 152 citations

Domain organization of chicken gizzard myosin light chain kinase deduced from a cloned cDNA

1986 • 148 citations

Phosphorylation of smooth muscle myosin light chain kinase by protein kinase C. Comparative study of the phosphorylated sites.

1985 • 145 citations

Chemical synthesis and expression of a calmodulin gene designed for site-specific mutagenesis

1985 • 140 citations

Multifunctional Ca2+/calmodulin-dependent protein kinase: domain structure and regulation

1989 • 136 citations

Site-specific mutagenesis of the alpha-helices of calmodulin. Effects of altering a charge cluster in the helix that links the two halves of calmodulin.

1987 • 135 citations

Isolation of the native form of chicken gizzard myosin light-chain kinase

1984 • 125 citations

Rabbit skeletal muscle myosin light chain kinase. The calmodulin binding domain as a potential active site-directed inhibitory domain.

1987 • 122 citations

Purification and characterization of a basal body-associated Ca2+-binding protein.

1988 • 112 citations

Identification of a cDNA clone that contains the complete coding sequence for a 140-kD rat NCAM polypeptide.

1987 • 110 citations

Calmodulin and the regulation of the actin—myosin interaction in smooth muscle and nonmuscle cells

1982 • 108 citations

Amino acid sequence specificities of an adhesive recognition signal

1985 • 106 citations

Structural organization, DNA sequence, and expression of the calmodulin gene.

1988 • 103 citations

Calcium-independent myosin light chain kinase of smooth muscle. Preparation by limited chymotryptic digestion of the calcium ion dependent enzyme, purification and characterization

1982 • 101 citations

Identification in Turkey Gizzard of an Acidic Protein Related to the C-terminal Portion of Smooth Muscle Myosin Light Chain Kinase

1989 • 97 citations

Amino acid sequence of rabbit skeletal muscle myosin light chain kinase

1986 • 96 citations

Characterization of antibodies to smooth muscle myosin kinase and their use in localizing myosin kinase in nonmuscle cells.

1981 • 89 citations

Partial deduced sequence of the 110-kD-calmodulin complex of the avian intestinal microvillus shows that this mechanoenzyme is a member of the myosin I family.

1989 • 89 citations

Calcium-sensitive regulation of actin-myosin interactions in baby hamster kidney (BHK-21) cells.

1979 • 83 citations

Pseudosubstrates turn off protein kinases

1988 • 81 citations

Calcium control of actin-myosin based contraction in triton models of mouse 3T3 fibroblasts is mediated by the myosin light chain kinase (MLCK)-calmodulin complex

1983 • 78 citations

Organelle Movements along Actin Filaments and Microtubules

1986 • 77 citations

Phosphorylation of smooth muscle myosin light chain kinase by Ca2+/calmodulin-dependent protein kinase II: Comparative study of the phosphorylation sites

1990 • 76 citations

Amino acid sequence of a novel calmodulin from Paramecium tetraurelia that contains dimethyllysine in the first domain.

1987 • 72 citations

Identification of calmodulin-binding proteins in chicken embryo fibroblasts.

1984 • 65 citations

Analytical subcellular distribution of calmodulin and calmodulin-binding proteins in normal and virus-transformed fibroblasts.

1983 • 64 citations

Membrane‐bound myosin‐l provides new mechanisms in cell motility

1989 • 64 citations

Mapping of calmodulin-binding domain of Ca2+/calmodulin-dependent protein kinase II from rat brain

1988 • 59 citations

Isolation of the cDNA encoding rat skeletal muscle myosin light chain kinase. Sequence and tissue distribution.

1988 • 56 citations

Isolation of cDNA clones for the catalytic gamma subunit of mouse muscle phosphorylase kinase: expression of mRNA in normal and mutant Phk mice.

1987 • 55 citations

Location of the Inhibitory Region of Smooth Muscle Myosin Light Chain Kinase

1989 • 55 citations

Computational and site‐specific mutagenesis analyses of the asymmetric charge distribution on calmodulin

1989 • 55 citations

Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of phosphorylase kinase

1987 • 53 citations

Domain characterization of rabbit skeletal muscle myosin light chain kinase.

1990 • 52 citations

Skeletal muscle phosphorylase kinase catalytic subunit mRNAs are expressed in heart tissue but not in liver.

1987 • 48 citations

In vivo mutations of calmodulin: a mutant Paramecium with altered ion current regulation has an isoleucine-to-threonine change at residue 136 and an altered methylation state at lysine residue 115.

1989 • 33 citations

Ca²⁺‐dependent contraction of human lung fibroblasts treated with triton X‐100: A role of Ca²⁺‐calmodulin‐dependent phosphorylation of myosin 20,000‐dalton light chain

1984 • 32 citations

Random phosphorylation of the two heads of thymus myosin and the independent stimulation of their actin-activated ATPases.

1985 • 31 citations

Smooth muscle myosin light chain kinase. Amino acid sequence at the site phosphorylated by adenosine cyclic 3',5'-phosphate-dependent protein kinase whether or not calmodulin is bound.

1986 • 31 citations

Sequence of the cDNA for the alpha subunit of calmodulin kinase II from mouse brain

1989 • 29 citations

Myosin Light Chain Kinase Structure Function Analysis Using Bacterial Expression

1989 • 27 citations

Regulation of muscle differentiation: isolation and purification of chick actin messenger ribonucleic acid and quantitation with complementary deoxyribonucleic acid probes

1980 • 26 citations

Evidence for regulation of lamellipodial and tail contraction of glycerinated chicken embryonic fibroblasts by myosin light chain kinase

1986 • 26 citations

Calmodulin and Calmodulin-regulated Protein Kinases as Transducers of Intracellular Calcium Signals

1988 • 24 citations

Nucleotide sequence of cDNA encoding the catalytic subunit of phosphorylase kinase from rat soleus muscle

1988 • 21 citations

[26] Purification of calmodulin and preparation of immobilized calmodulin

1988 • 17 citations

Tryptic digestion of myosin light chain kinase produces an inactive fragment that is activated on continued digestion

1986 • 16 citations

Modulation of the stability of rabbit skeletal muscle myosin light chain kinase through the calmodulin-binding domain.

1990 • 16 citations

Perturbation of the Calmodulin System in Transformed Cells

1990 • 15 citations

Purification and characterization of myosin light-chain kinase from the rat pancreas

1989 • 15 citations

Immunological properties of myosin light-chain kinases

1987 • 14 citations

Molecular characterization of rat skeletal muscle myosin light chain kinase

1989 • 14 citations

Immunoreactive levels of myosin light-chain kinase in normal and virus-transformed chicken embryo fibroblasts.

1984 • 14 citations

Nonmuscle myosin phosphorylation sites for calcium-dependent and calcium-independent protein kinases

1986 • 13 citations

Identification of the native form of chicken gizzard myosin light chain kinase with the aid of monoclonal antibodies

1983 • 12 citations

Calcium-dependent shortening of fibroblasts induced by the ionophore, a23187

1981 • 11 citations

AN INTERDISCIPLINARY APPROACH TO THE MOLECULAR MECHANISMS OF CALMODULIN ACTION: COMPARATIVE BIOCHEMISTRY, SITE-SPECIFIC MUTAGENESIS, AND PROTEIN ENGINEERING

1987 • 10 citations

Identification of two forms of myosin light chain kinase in turkey gizzard

1983 • 6 citations

Immunoreactive Levels of Myosin Light-Chain Kinase in Normal and Virus-Transformed Chicken Embryo Fibroblasts

1984 • 3 citations

Smooth Muscle Myosin Light Chain Kinase

2017 • 2 citations