The essential OST2 gene encodes the 16-kD subunit of the yeast oligosaccharyltransferase, a highly conserved protein expressed in diverse eukaryotic organisms.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Basic local alignment search tool

1990 • 87,358 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Antibodies, a laboratory manual

1989 • 12,929 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

ASSEMBLY OF ASPARAGINE-LINKED OLIGOSACCHARIDES

1985 • 4,790 citations

The scanning model for translation: an update.

1989 • 3,525 citations

Getting started with yeast

2002 • 3,496 citations

Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3Å resolution

1985 • 3,049 citations

A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coli

1987 • 2,493 citations

[1] Getting started with yeast

1991 • 2,456 citations

Multifunctional yeast high-copy-number shuttle vectors

1992 • 1,656 citations

A Model Recognition Approach to the Prediction of All-Helical Membrane Protein Structure and Topology

1994 • 790 citations

Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: implications for protein engineering

1990 • 750 citations

Toward an understanding of the molecular mechanisms of physiological cell death.

1993 • 623 citations

How N-linked oligosaccharides affect glycoprotein folding in the endoplasmic reticulum.

1994 • 617 citations

A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction

1989 • 614 citations

Chitinase is required for cell separation during growth of Saccharomyces cerevisiae.

1991 • 601 citations

Predicting the orientation of eukaryotic membrane-spanning proteins.

1989 • 596 citations

[20] In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast

1991 • 584 citations

Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole

1982 • 579 citations

Plasmid construction by homologous recombination in yeast

1987 • 560 citations

Glycoprotein biosynthesis in yeast

1993 • 514 citations

Sequence specificity in the dimerization of transmembrane .alpha.-helixes

1992 • 490 citations

Genetic analysis of yeast RAS1 and RAS2 genes

1984 • 460 citations

PROTEIN GLYCOSYLATION IN YEAST

1987 • 437 citations

Hydrophobic Organization of Membrane Proteins

1989 • 316 citations

Oligosaccharyltransferase activity is associated with a protein complex composed of ribophorins I and II and a 48 kd protein

1992 • 291 citations

Membrane protein association by potential intrarnembrane charge pairs

1991 • 239 citations

Cloning and sequencing of the yeast gene for dolichol phosphate mannose synthase, an essential protein.

1988 • 231 citations

Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells.

1993 • 223 citations

Transmembrane Helical Interactions and the Assembly of the T Cell Receptor Complex

1990 • 217 citations

Cloning of Saccharomyces cerevisiae DNA replication genes: isolation of the CDC8 gene and two genes that compensate for the cdc8-1 mutation.

1983 • 192 citations

STT3, a highly conserved protein required for yeast oligosaccharyl transferase activity in vivo.

1995 • 187 citations

Guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen of Saccharomyces cerevisiae

1993 • 183 citations

GENETIC ANALYSIS OF THE YEAST CYTOSKELETON

1987 • 179 citations

Separation of neutral oligosaccharides by high-performance liquid chromatography

1981 • 169 citations

The yeast WBP1 is essential for oligosaccharyl transferase activity in vivo and in vitro.

1992 • 156 citations

Glycosylation allows cell-surface transport of an anchored secretory protein

1985 • 145 citations

Yeast Wbp1p and Swp1p form a protein complex essential for oligosaccharyl transferase activity.

1993 • 125 citations

Saccharomyces cerevisiae mutants that make mannoproteins with a truncated carbohydrate outer chain.

1980 • 117 citations

Isolation of the ALG5 Locus Encoding the UDP‐Glucose:Dolichyl‐Phosphate Glucosyltransferase from Saccharomyces cerevisiae

1994 • 105 citations

The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides.

1994 • 105 citations

New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: cloning of the ALG8 locus.

1994 • 99 citations

Two yeast mutations in glucosylation steps of the asparagine glycosylation pathway.

1984 • 94 citations

The 48-kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1.

1992 • 88 citations

Chapter 1 A Hitchhiker's Guide to Analysis of the Secretory Pathway in Yeast

1989 • 88 citations

Yeast carboxypeptidase Y requires glycosylation for efficient intracellular transport, but not for vacuolar sorting, in vivo stability, or activity

1991 • 83 citations

Removal of N-glycosylation sites of the yeast acid phosphatase severely affects protein folding

1991 • 78 citations

Functional characterization of Ost3p. Loss of the 34-kD subunit of the Saccharomyces cerevisiae oligosaccharyltransferase results in biased underglycosylation of acceptor substrates.

1995 • 77 citations

Inhibition of N‐linked glycosylation induces early apoptosis in human promyelocytic HL‐60 cells

1995 • 73 citations

An essential 45 kDa yeast transmembrane protein reacts with anti-nuclear pore antibodies: purification of the protein, immunolocalization and cloning of the gene.

1991 • 72 citations

The alpha subunit of the Saccharomyces cerevisiae oligosaccharyltransferase complex is essential for vegetative growth of yeast and is homologous to mammalian ribophorin I.

1995 • 68 citations

The N‐oligosaccharyltransferase complex from yeast

1994 • 68 citations

Another brick in the wall? Recent developments concerning the yeast cell envelope

1994 • 66 citations

[33] Contranslational amino-terminal processing

1990 • 61 citations

Molecular Cloning of a Human cDNA Encoding a Novel Protein, DAD1, Whose Defect Causes Apoptotic Cell Death in Hamster BHK21 Cells

1993 • 60 citations

Cloning of Saccharomyces cerevisiae DNA Replication Genes: Isolation of the CDC8 Gene and Two Genes That Compensate for the cdc8-1 Mutation

1983 • 60 citations

Sulfhydryl Modification of the Yeast Wbp1p Inhibits Oligosaccharyl Transferase Activity

1995 • 57 citations

Purification and characterization of avian oligosaccharyltransferase. Complete amino acid sequence of the 50-kDa subunit.

1994 • 44 citations

Diminished activity of the first N-glycosylation enzyme, dolichol-P-dependent N-acetylglucosamine-1-P transferase (GPT), gives rise to mutant phenotypes in yeast

1995 • 34 citations

The essential yeast NLT1 gene encodes the 64 kDa glycoprotein subunit of the oligosaccharyl transferase

1995 • 28 citations

An essential 45 kDa yeast transmembrane protein reacts with anti-nuclear pore antibodies: purification of the protein, immunolocalization and cloning of the gene.

1991 • 24 citations