Site-Specific Initiation of DNA Replication in Xenopus Egg Extract Requires Nuclear Structure

Genomic sequencing.

1984 • 8,343 citations

Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors.

1990 • 909 citations

Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs

1986 • 622 citations

Two steps in the assembly of complexes at yeast replication origins in vivo

1994 • 566 citations

Nuclear reconstitution in vitro: Stages of assembly around protein-free DNA

1987 • 550 citations

Mapping replicational sites in the eucaryotic cell nucleus.

1989 • 520 citations

The Units of DNA Replication in Drosophila melanogaster Chromosomes

1974 • 518 citations

EUKARYOTIC DNA POLYMERASES

1991 • 493 citations

Dynamic organization of DNA replication in mammalian cell nuclei: spatially and temporally defined replication of chromosome-specific alpha-satellite DNA sequences.

1992 • 478 citations

Visualization of replication factories attached to a nucleoskeleton

1993 • 472 citations

A lamin-independent pathway for nuclear envelope assembly.

1990 • 350 citations

The events of the midblastula transition in Xenopus are regulated by changes in the cell cycle

1987 • 312 citations

A method for incorporating macromolecules into adherent cells.

1984 • 307 citations

Identification of an origin of bidirectional DNA replication in mammalian chromosomes

1990 • 288 citations

EUKARYOTIC DNA REPLICATION: Anatomy of An Origin

1993 • 280 citations

Replication structure of the human & beta;-globin gene domain

1993 • 277 citations

Initiation and termination of DNA replication in human rRNA genes.

1993 • 247 citations

Electron microscopic analysis of chromatin replication in the cellular blastoderm drosophila melanogaster embryo

1977 • 234 citations

Mapping replication units in animal cells

1989 • 232 citations

Replication occurs at a nucleoskeleton.

1986 • 230 citations

Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis

1985 • 227 citations

Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos.

1993 • 215 citations

Changes in the nuclear lamina composition during early development of Xenopus laevis

1985 • 214 citations

A relationship between replicon size and supercoiled loop domains in the eukaryotic genome

1982 • 209 citations

Origins of DNA replication in metazoan chromosomes.

1993 • 203 citations

Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor

1993 • 198 citations

Fine mapping of a replication origin of human DNA.

1994 • 193 citations

Matrix attachment regions are positioned near replication initiation sites, genes, and an interamplicon junction in the amplified dihydrofolate reductase domain of Chinese hamster ovary cells.

1988 • 177 citations

REGULATION OF EUKARYOTIC DNA REPLICATION

1994 • 170 citations

Spatial and temporal distribution of DNA replication sites localized by immunofluorescence and confocal microscopy in mouse fibroblasts

1991 • 170 citations

Competition between chromatin and transcription complex assembly regulates gene expression during early development

1994 • 159 citations

DNA replication initiates at multiple sites on plasmid DNA in Xenopus egg extracts

1992 • 154 citations

Emetine allows identification of origins of mammalian DNA replication by imbalanced DNA synthesis, not through conservative nucleosome segregation.

1991 • 140 citations

The nuclear membrane prevents replication of human G2 nuclei but not G1 nuclei in Xenopus egg extract

1992 • 140 citations

Mimosine Arrests DNA Synthesis at Replication Forks by Inhibiting Deoxyribonucleotide Metabolism

1995 • 139 citations

Stability of RNA in developing Xenopus embryos and identification of a destabilizing sequence in TFIIIA messenger RNA

1988 • 138 citations

Distinct roles of cdk2 and cdc2 in RP-A phosphorylation during the cell cycle

1993 • 136 citations

Bovine papilloma virus plasmids replicate randomly in mouse fibroblasts throughout S phase of the cell cycle

1987 • 135 citations

High-resolution mapping of replication fork movement through the amplified dihydrofolate reductase domain in CHO cells by in-gel renaturation analysis.

1989 • 134 citations

Early dihydrofolate reductase gene amplification events in CHO cells usually occur on the same chromosome arm as the original locus.

1989 • 132 citations

Replication in the amplified dihydrofolate reductase domain in CHO cells may initiate at two distinct sites, one of which is a repetitive sequence element.

1989 • 130 citations

Intramolecular DNA triplexes, bent DNA and DNA unwinding elements in the initiation region of an amplified dihydrofolate reductase replicon

1990 • 129 citations

Initiation of DNA replication in the dihydrofolate reductase locus is confined to the early S period in CHO cells synchronized with the plant amino acid mimosine.

1992 • 128 citations

Study of the cell cycle-dependent assembly of the DNA pre-replication centres in Xenopus egg extracts.

1994 • 124 citations

Two DNA polymerases may be required for synthesis of the lagging DNA strand of simian virus 40

1990 • 121 citations

Ease of DNA unwinding is a conserved property of yeast replication origins

1993 • 119 citations

Intracellular localization and metabolism of DNA polymerase α in human cells visualized with monoclonal antibody

1984 • 115 citations

[19] Cell cycle analysis by flow cytometry

1979 • 114 citations

Drosophila chorion genes: Cracking the eggshell's secrets

1991 • 109 citations

Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix

1993 • 108 citations

The timing of the formation and usage of replicase clusters in s-phase nuclei of human diploid fibroblasts

1991 • 101 citations

Replication of purified DNA in Xenopus egg extract is dependent on nuclear assembly

1990 • 100 citations

Analysis of chromosomal replicons in early embryos ofDrosophila melanogasterby two-dimensional gel electrophoresis

1991 • 92 citations

Structure of the dihydrofolate reductase gene in Chinese hamster ovary cells

1983 • 87 citations

Autonomous Replication in Human Cells of Multimers of Specific Human and Bacterial DNA Sequences

1993 • 87 citations

The termination region for SV40 DNA replication directs the mode of separation for the two sibling molecules

1985 • 84 citations

Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts.

1993 • 78 citations

Modular sequence elements associated with origin regions in eukaryotic chromosomal DNA

1994 • 78 citations

Localization and DNA sequence of a replication origin in the rhodopsin gene locus of Chinese hamster cells

1992 • 76 citations

Initiation of Simian Virus 40 DNA Replication in Vitro: Aphidicolin Causes Accumulation of Early-Replicating Intermediates and Allows Determination of the Initial Direction of DNA Synthesis

1986 • 71 citations

Mapping of Replication Initiation Sites in Human Ribosomal DNA by Nascent-Strand Abundance Analysis

1995 • 67 citations

Immunoglobulin heavy chain enhancer is located near or in an initiation zone of chromosomal DNA replication.

1993 • 63 citations

Transcription inhibits the replication of autonomously replicating plasmids in human cells.

1994 • 63 citations

Replication of Ribosomal DNA in Xenopus laevis

1981 • 60 citations

Densely methylated DNA islands in mammalian chromosomal replication origins.

1994 • 59 citations

Localization of a bidirectional DNA replication origin in the native locus and in episomally amplified murine adenosine deaminase loci.

1993 • 58 citations

In VitroReplication of Plasmids Containing Human c-mycDNA

1995 • 57 citations

Replication initiation sites are distributed widely in the amplified CHO dihydrofolate reductase domain

1994 • 54 citations

DNA replication in cell-free extracts from Xenopus eggs is prevented by disrupting nuclear envelope function

1992 • 53 citations

A novel DNA replication origin identified in the human heat shock protein 70 gene promoter.

1994 • 51 citations

Analysis of the autonomous replication behavior in human cells of the dihydrofolate reductase putative chromosomal origin of replication

1992 • 49 citations

The role of transcription factors, chromatin structure and DNA replication in 5 S RNA gene regulation

1994 • 49 citations

DNA Helical Instability Facilitates Initiation at the SV40 Replication Origin

1994 • 46 citations

Analysis of a replication initiation sequence from the adenosine deaminase region of the mouse genome.

1993 • 45 citations

Analysis of an origin of DNA amplification in Sciara coprophila by a novel three-dimensional gel method.

1994 • 43 citations

DNA Replication Origins in Animal Cells: a Question of Context?

1994 • 40 citations

Nuclear DNA synthesis in vitro is mediated via stable replication forks assembled in a temporally specific fashion in vivo.

1988 • 40 citations

Structural analysis of the mitotic cycle in pre-gastrula Xenopus embryos

1988 • 39 citations

Identification of an Origin of Bidirectional DNA Replication in the Ubiquitously Expressed Mammalian CAD Gene

1995 • 39 citations

Mapping an initiation region of DNA replication at a single-copy chromosomal locus in Drosophila melanogaster cells by two-dimensional gel methods and PCR-mediated nascent-strand analysis: multiple replication origins in a broad zone.

1994 • 38 citations

A Mammalian Origin of Bidirectional DNA Replication within the Chinese HamsterRPS14Locust

1994 • 38 citations

Autonomous replication of human chromosomal DNA fragments in human cells.

1993 • 38 citations

Antifolate-resistant Chinese hamster cells. Molecular basis for the biochemical and structural heterogeneity among dihydrofolate reductases produced by drug-sensitive and drug-resistant cell lines.

1988 • 34 citations

Ligand dependence of estrogen receptor induced changes in chromatin structure

1992 • 31 citations

Nuclear distribution of PCNA during embryonic development in Xenopus laevis: a reinvestigation of early cell cycles

1992 • 28 citations

Tight association of DNA polymerase .ALPHA. with granular structures in the nuclear matrix of chick embryo cell: Immunocytochemical detection with monoclonal antibody against DNA polymerase .ALPHA..

1984 • 25 citations

In vivo activity for initiation of DNA replication resides in a transcribed region of the human genome

1993 • 25 citations

Site-specific Initiation of DNA Replication in Metazoan Chromosomes and the Role of Nuclear Organization

1993 • 24 citations

DNA replication from initiation zones of mammalian cells in a model system.

1994 • 23 citations

Purification of Circular DNA Using Benzoylated Naphthoylated DEAE-Cellulose

1985 • 21 citations

Densely Methylated DNA Islands in Mammalian Chromosomal Replication Origins

1994 • 20 citations

Aphidicolin-induced topological and recombinational events in simian virus 40

1991 • 19 citations

Aphidicolin arrest irreversibly impairs replicating simian virus 40 chromosomes.

1983 • 19 citations

Analysis of a Replication Initiation Sequence from the Adenosine Deaminase Region of the Mouse Genome

1993 • 18 citations

Mapping an Initiation Region of DNA Replication at a Single-Copy Chromosomal Locus in Drosophila melanogaster Cells by Two-Dimensional Gel Methods and PCR-Mediated Nascent-Strand Analysis: Multiple Replication Origins in a Broad Zone

1994 • 14 citations

Patterns of dna replication in Drosophila polytene nuclei replicating in Xenopus egg and oocyte extracts

1992 • 11 citations

[47] Specific labeling of newly replicated DNA

1995 • 10 citations

DNA Replication from Initiation Zones of Mammalian cells in a Model System

1994 • 6 citations