Additive Attenuation of Virulence of Streptococcus pneumoniae by Mutation of the Genes Encoding Pneumolysin and Other Putative Pneumococcal Virulence Proteins

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES

1944 • 3,183 citations

Changes in size and secondary structure of the ribosomal transcription unit during vertebrate evolution

1975 • 1,188 citations

Pneumococcal resistance to antibiotics

1990 • 820 citations

Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types

2017 • 770 citations

Contribution of novel choline‐binding proteins to adherence, colonization and immunogenicity of Streptococcus pneumoniae

1997 • 488 citations

Conservation of a hexapeptide sequence in the anchor region of surface proteins from Gram‐positive cocci

1990 • 412 citations

Antibody Response to Pneumococcal Vaccination in Children Younger than Five Years of Age

1983 • 384 citations

Competence and virulence of Streptococcus pneumoniae: Adc and PsaA mutants exhibit a requirement for Zn and Mn resulting from inactivation of putative ABC metal permeases

1997 • 383 citations

SpsA, a novel pneumococcal surface protein with specific binding to secretory Immunoglobulin A and secretory component

1997 • 336 citations

Reduced virulence of a defined pneumolysin-negative mutant of Streptococcus pneumoniae

1989 • 335 citations

Contribution of autolysin to virulence of Streptococcus pneumoniae

1989 • 261 citations

Sequence heterogeneity of PsaA, a 37-kilodalton putative adhesin essential for virulence of Streptococcus pneumoniae

1996 • 261 citations

MOLECULAR ANALYSIS OF THE PATHOGENICITY OF STREPTOCOCCUS PNEUMONIAE: The Role of Pneumococcal Proteins

1993 • 259 citations

Intranasal Immunization of Mice with PspA (Pneumococcal Surface Protein A) Can Prevent Intranasal Carriage, Pulmonary Infection, and Sepsis withStreptococcus pneumoniae

1997 • 247 citations

Immunization of mice with pneumolysin toxoid confers a significant degree of protection against at least nine serotypes of Streptococcus pneumoniae

1994 • 226 citations

Novel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning

1983 • 225 citations

Activation of human complement by the pneumococcal toxin pneumolysin

1984 • 220 citations

Structural properties and evolutionary relationships of PspA, a surface protein of Streptococcus pneumoniae, as revealed by sequence analysis

1992 • 220 citations

The crystal structure of pneumococcal surface antigen PsaA reveals a metal-binding site and a novel structure for a putative ABC-type binding protein

1998 • 219 citations

Use of insertional inactivation to facilitate studies of biological properties of pneumococcal surface protein A (PspA).

1987 • 214 citations

Effect of immunization with pneumolysin on survival time of mice challenged with Streptococcus pneumoniae

1983 • 205 citations

Identification of Pneumococcal Surface Protein A as a Lactoferrin-Binding Protein of Streptococcus pneumoniae

1999 • 188 citations

Molecular evolution of lytic enzymes of Streptococcus pneumoniae and its bacteriophages.

1988 • 174 citations

Nucleotide sequence and expression of the pneumococcal autolysin gene from its own promoter in Escherichia coli

1986 • 167 citations

Pneumococcal Diversity: Considerations for New Vaccine Strategies with Emphasis on Pneumococcal Surface Protein A (PspA)

1998 • 160 citations

A neuraminidase from Streptococcus pneumoniae has the features of a surface protein

1994 • 142 citations

The contribution of pneumolysin to the pathogenicity of Streptococcus pneumoniae

1996 • 136 citations

Interruption of capsule production in Streptococcus pneumonia serotype 3 by insertion of transposon Tn916

1990 • 134 citations

Structure and function of pneumolysin, the multifunctional, thiol‐activated toxin of Streptococcus pneumoniae

1991 • 126 citations

Destruction of low efficiency markers is a slow process occurring at a heteroduplex stage of transformation

1974 • 125 citations

Penicillin tolerance genes ofStreptococcus pneumoniae: the ABC‐type manganese permease complex Psa

1998 • 117 citations

Truncated forms of PspA that are secreted from Streptococcus pneumoniae and their use in functional studies and cloning of the pspA gene

1992 • 116 citations

Cloning and nucleotide sequence of the Streptococcus pneumoniae hyaluronidase gene and purification of the enzyme from recombinant Escherichia coli

1994 • 113 citations

Comparative efficacy of pneumococcal neuraminidase and pneumolysin as immunogens protective against Streptococcus pneumoniae

1988 • 110 citations

Transformation of encapsulated Streptococcus pneumoniae

1986 • 107 citations

Effect of defined point mutations in the pneumolysin gene on the virulence of Streptococcus pneumoniae

1995 • 103 citations

Cloning and characterization of nanB, a second Streptococcus pneumoniae neuraminidase gene, and purification of the NanB enzyme from recombinant Escherichia coli

1996 • 92 citations

Comparative efficacy of autolysin and pneumolysin as immunogens protecting mice against infection by Streptococcus pneumoniae

1992 • 86 citations

Effect of insertional inactivation of the genes encoding pneumolysin and autolysin on the virulence of Streptococcus pneumoniae type 3

1992 • 83 citations

Comparative Virulence of Streptococcus pneumoniae Strains with Insertion-Duplication, Point, and Deletion Mutations in the Pneumolysin Gene

1999 • 82 citations

Slow excision repair in an mfd mutant of Escherichia coli B/r

1974 • 79 citations

Novel pneumococcal surface proteins: role in virulence and vaccine potential

1998 • 76 citations

Molecular Analysis of Putative Pneumococcal Virulence Proteins

1997 • 75 citations

Systemic and Mucosal Protective Immunity to Pneumococcal Surface Protein A

1996 • 62 citations

Purification and immunological characterization of neuraminidase produced by Streptococcus pneumoniae

1988 • 55 citations

Analysis of pneumococcal PspA microheterogeneity in SDS polyacrylamide gels and the association of PspA with the cell membrane

1992 • 32 citations