Rational Design of an Active Avidin Monomer

The "megaprimer" method of site-directed mutagenesis.

1990 • 1,174 citations

Structural Origins of High-Affinity Biotin Binding to Streptavidin

1989 • 1,162 citations

[5] Avidin and streptavidin

1990 • 1,005 citations

Three-dimensional structures of avidin and the avidin-biotin complex.

1993 • 637 citations

Crystal structure of core streptavidin determined from multiwavelength anomalous diffraction of synchrotron radiation.

1989 • 579 citations

Three-dimensional Structure of the Tetragonal Crystal Form of Egg-white Avidin in its functional Complex with Biotin at 2·7 Å Resolution

1993 • 300 citations

Site-directed mutagenesis studies of the high-affinity streptavidin-biotin complex: contributions of tryptophan residues 79, 108, and 120.

1995 • 201 citations

Molecular Recognition between Genetically Engineered Streptavidin and Surface-Bound Biotin

1999 • 182 citations

Kinetics of Protein-Protein Interactions at the Surface of an Optical Biosensor

1995 • 178 citations

Intersubunit contacts made by tryptophan 120 with biotin are essential for both strong biotin binding and biotin-induced tighter subunit association of streptavidin.

1995 • 135 citations

The properties of subunits of avidin coupled to Sepharose

1973 • 131 citations

A tetravalent single-chain antibody-streptavidin fusion protein for pretargeted lymphoma therapy.

2000 • 127 citations

Kinetic analysis of biosensor data: elementary tests for self-consistency

1996 • 126 citations

Recombinant NeutraLite Avidin: a non‐glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non‐specific binding properties

2000 • 107 citations

Sodium dodecyl sulfate‐polyacrylamide gel electrophoretic method for assessing the quaternary state and comparative thermostability of avidin and streptavidin

1996 • 103 citations

An avidin monomer affinity column for the purification of biotin-containing enzymes

1979 • 103 citations

Development and Characterization of a Series of Soluble Tetrameric and Monomeric Streptavidin Muteins with Differential Biotin Binding Affinities

2001 • 99 citations

The quaternary structure of streptavidin in urea

1991 • 75 citations

[20] Monovalent avidin affinity columns

1990 • 68 citations

Engineering subunit association of multisubunit proteins: A dimeric streptavidin

1997 • 68 citations

Mutation of a critical tryptophan to lysine in avidin or streptavidin may explain why sea urchin fibropellin adopts an avidin‐like domain

1999 • 58 citations

Production of Biologically Active Recombinant Avidin in Baculovirus-Infected Insect Cells

1997 • 58 citations

Biotin Induces Tetramerization of a Recombinant Monomeric Avidin

2001 • 57 citations

Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin

2002 • 53 citations

Limited proteolysis of native proteins: The interaction between avidin and proteinase K

1995 • 51 citations

Engineering of chicken avidin: a progressive series of reduced charge mutants

1998 • 43 citations

The Major Conformational IgE-binding Epitopes of Hevein (Hev b6.02) Are Identified by a Novel Chimera-based Allergen Epitope Mapping Strategy

2002 • 41 citations

X-ray crystallographic studies of streptavidin mutants binding to biotin

1999 • 31 citations

Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin

2002 • 31 citations

Avidin Is a Promising Tag for Fusion Proteins Produced in Baculovirus-Infected Insect Cells

1999 • 29 citations

[17] 2-Iminobiotin-containing reagent and affinity columns

1990 • 25 citations

Evolution of the fibropellin gene family and patterns of fibropellin gene expression in sea urchin phylogeny

1995 • 19 citations