Depletion of yeast RNase III blocks correct U2 3' end formation and results in polyadenylated but functional U2 snRNA

Methods in yeast genetics

1979 • 7,824 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Guide to Yeast Genetics and Molecular Biology

1993 • 2,377 citations

Methods in Yeast Genetics: A Laboratory Course Manual

1990 • 1,922 citations

Multifunctional yeast high-copy-number shuttle vectors

1992 • 1,656 citations

Methods in Yeast Genetics — A Laboratory Course Manual

1991 • 1,257 citations

Mechanical Devices of the Spliceosome: Motors, Clocks, Springs, and Things

1998 • 1,121 citations

Guide to Yeast Genetics and Molecular Biology

1991 • 607 citations

SPLICEOSOMAL snRNAs

1988 • 463 citations

Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing

1989 • 391 citations

Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles

1988 • 363 citations

A practical guide to molecular cloning

1987 • 360 citations

DYNAMIC RNA-RNA INTERACTIONS IN THE SPLICEOSOME

1994 • 356 citations

Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, U2, U4 and U5 snRNPs.

1995 • 294 citations

Formation of the 3′ end of U1 snRNA requires compatible snRNA promoter elements

1986 • 255 citations

snRNP Sm proteins share two evolutionarily conserved sequence motifs which are involved in Sm protein-protein interactions.

1995 • 248 citations

RNase III Cleaves Eukaryotic Preribosomal RNA at a U3 snoRNP-Dependent Site

1996 • 230 citations

3′ end formation of U1 snRNA precursors is coupled to transcription from snRNA promoters

1986 • 222 citations

Formation of the 3′ end of U1 snRNA is directed by a conserved sequence located downstream of the coding region.

1985 • 183 citations

An essential yeast snRNA with a U5-like domain is required for splicing in vivo

1987 • 168 citations

Cell Biology of the snRNP Particle

1990 • 163 citations

U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs

1986 • 160 citations

Lethal and temperature-sensitive mutations and their suppressors identify an essential structural element in U2 small nuclear RNA.

1990 • 147 citations

UsnRNP Assembly and Transport

1988 • 142 citations

Yeast contains small nuclear RNAs encoded by single copy genes

1983 • 133 citations

Sequences required for 3′ end formation of human U2 small nuclear RNA

1985 • 122 citations

Polyadenylation of telomerase RNA in budding yeast.

1997 • 119 citations

Alternative 3′-end processing of U5 snRNA by RNase III

1997 • 114 citations

Unexpected flexibility in an evolutionarily conserved protein-RNA interaction: genetic analysis of the Sm binding site.

1990 • 95 citations

Two conserved domains of yeast U2 snRNA are separated by 945 nonessential nucleotides

1988 • 93 citations

In vitro reconstitution of functional yeast U2 snRNPs.

1989 • 91 citations

Nucleocytoplasmic transport and processing of small nuclear RNA precursors.

1990 • 90 citations

In vitro reconstitution of mammalian U2 and U5 snRNPs active in splicing: Sm proteins are functionally interchangeable and are essential for the formation of functional U2 and U5 snRNPs.

1995 • 79 citations

Rearrangement of snRNA Structure during Assembly and Function of the Spliceosome

1995 • 75 citations

Internal sequences that distinguish yeast from metazoan U2 snRNA are unnecessary for pre-mRNA splicing

1988 • 66 citations

Invariant U2 RNA Sequences Bordering the Branchpoint Recognition Region Are Essential for Interaction with Yeast SF3a and SF3b Subunits

1996 • 65 citations

Structurally Related but Functionally Distinct Yeast Sm D Core Small Nuclear Ribonucleoprotein Particle Proteins

1995 • 63 citations

Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae.

1994 • 56 citations

Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly

1991 • 54 citations

A conserved sequence element in ribonuclease III processing signals is not required for accuratein vitroenzymatic cleavage

1991 • 44 citations

Nucleocytoplasmic Transport and Processing of Small Nuclear RNA Precursors

1990 • 37 citations

Rescue of the fission yeast snRNA synthesis mutant snm1 by overexpression of the double-strand-specific Pac1 ribonuclease

1995 • 34 citations

Limited functional equivalence of phylogenetic variation in small nuclear RNA: yeast U2 RNA with altered branchpoint complementarity inhibits splicing and produces a dominant lethal phenotype.

1991 • 32 citations

Human snRNP polypeptide D1 promotes pre-mRNA splicing in yeast and defines nonessential yeast Smd1p sequences

1993 • 25 citations

Interactions between Highly Conserved U2 Small Nuclear RNA Structures and Prp5p, Prp9p, Prp11p, and Prp21p Proteins Are Required To Ensure Integrity of the U2 Small Nuclear Ribonucleoprotein in Saccharomyces cerevisiae

1994 • 22 citations

Dynamic RNA-RNA Interactions in the Spliceosome

1994 • 22 citations

A mutation in a single gene of Schizosaccharomyces pombe affects the expression of several snRNAs and causes defects in RNA processing.

1990 • 21 citations

Structure and expression of U-snRNA genes

1987 • 12 citations