The gene encoding streptothricin acetyltransferase (sat) as a selectable marker for Leishmania expression vectors

Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression.

1990 • 450 citations

Double targeted gene replacement for creating null mutants.

1991 • 323 citations

Gene replacement in parasitic protozoa

1990 • 238 citations

Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes.

1990 • 222 citations

A shuttle vector which facilitates the expression of transfected genes inTrypanosoma cruzi and Leishmania

1992 • 214 citations

Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts.

1993 • 189 citations

Targeted insertion of the neomycin phosphotransferase gene into the tubulin gene cluster of Trypanosoma brucei

1990 • 177 citations

Molecular cloning of the major surface antigen of leishmania.

1988 • 169 citations

Stable expression of the bacterial neor gene in Leishmania enriettii

1990 • 159 citations

Expression of a Bacterial Gene in a Trypanosomatid Protozoan

1989 • 149 citations

Homologous Recombination and Stable Transfection in the Parasitic Protozoan Trypanosoma brucei

1990 • 135 citations

Genes encoding the major surface glycoprotein in Leishmania are tandemly linked at a single chromosomal locus and are constitutively transcribed

1989 • 131 citations

Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.

1989 • 116 citations

Sequence and transcriptional analysis of the nourseothricin acetyltransferase-encoding gene nat1 from Streptomyces noursei

1993 • 81 citations

Two more independent selectable markers for stable transfection of Leishmania

1993 • 65 citations

Maturation of Polycistronic Pre-mRNA in Trypanosoma brucei: Analysis of trans Splicing and Poly(A) Addition at Nascent RNA Transcripts from the hsp70 Locus

1991 • 65 citations

The 63-kilobase circular amplicon of tunicamycin-resistant Leishmania amazonensis contains a functional N-acetylglucosamine-1-phosphate transferase gene that can be used as a dominant selectable marker in transfection.

1992 • 63 citations

Three selectable markers for transformation of Ustilago maydis

1994 • 50 citations

Stable transformation of Trypanosoma brucei

1993 • 46 citations

The hygromycin B-resistance-encoding gene as a selectable marker for stable transformation of Trypanosoma brucei

1991 • 43 citations

Thebleresistance gene as a new selectable marker forTrypanosoma brucei: fly transmission of stable procyclic transformants to produce antibiotic resistant bloodstream forms

1993 • 42 citations

Maturation of polycistronic pre-mRNA in Trypanosoma brucei: analysis of trans splicing and poly(A) addition at nascent RNA transcripts from the hsp70 locus.

1991 • 40 citations

Analysis of the nourseothricin-resistance gene (nat) of Streptomyces noursei

1988 • 38 citations

The nucleotide sequence ofleuAfromSalmonella typhimurium

1990 • 27 citations

Nucleotide sequence of a plasmid born streptothricin-acetyl-transferase gene (sat-1)

1989 • 22 citations

Leishmania major HEXBP deletion mutants generated by double targeted gene replacement

1994 • 20 citations

Nucleotide sequence of the streptothricin-acetyl-transferase gene sat-2

1990 • 14 citations