Targeted gene replacements in a Streptomyces polyketide synthase gene cluster: role for the acyl carrier protein

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

1977 • 3,829 citations

[1] Production of single-stranded plasmid DNA

1987 • 2,642 citations

Genetic Manipulation of Streptomyces — A Laboratory Manual

1986 • 1,304 citations

TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEA

1982 • 1,253 citations

Experiments with gene fusions

1985 • 1,210 citations

Construction and characterization of new cloning vehicles I. Ampicillin-resistant derivatives of the plasmid pMB9

1977 • 1,055 citations

Modular Organization of Genes Required for Complex Polyketide Biosynthesis

1991 • 883 citations

Fatty acid synthase, a proficient multifunctional enzyme

1989 • 801 citations

Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector

1992 • 714 citations

MOLECULAR GENETICS OF POLYKETIDES AND ITS COMPARISON TO FATTY ACID BIOSYNTHESIS

1990 • 674 citations

Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host

1984 • 358 citations

Analysis of the nucleotide sequence of the Streptomyces glaucescens tcmI genes provides key information about the enzymology of polyketide antibiotic biosynthesis.

1989 • 250 citations

Physical and genetical characterisation of a second sex factor, SCP2, for Streptomyces coelicolor A3(2)

1977 • 231 citations

Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin.

1992 • 229 citations

Structure and deduced function of the granaticin-producing polyketide synthase gene cluster of Streptomyces violaceoruber Tü22.

1989 • 226 citations

A vector system with temperature-sensitive replication for gene disruption and mutational cloning in streptomycetes

1989 • 223 citations

The Streptomyces plasmid SCP2∗: its functional analysis and development into useful cloning vectors

1985 • 208 citations

Genetics of Actinorhodin Biosynthesis by Streptomyces coelicolor A3(2)

1979 • 197 citations

Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin inStreptomyces coelicolor A3(2)

1986 • 187 citations

Spore colour in Streptomyces coelicolor A3(2) involves the developmentally regulated synthesis of a compound biosynthetically related to polyketide antibiotics

1990 • 184 citations

A combined genetic and physical map of the Streptomyces coelicolor A3(2) chromosome

1992 • 183 citations

The Complete Amino Acid Sequence of the Acyl Carrier Protein of Escherichia coli

1968 • 181 citations

Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor

1988 • 178 citations

The role of cysteines in polyketide synthases. Site-directed mutagenesis of resveratrol and chalcone synthases, two key enzymes in different plant-specific pathways

1991 • 175 citations

Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis

1988 • 163 citations

Biosynthesis of anthraquinones by interspecies cloning of actinorhodin biosynthesis genes in streptomycetes: clarification of actinorhodin gene functions

1990 • 157 citations

Cloning, disruption, and transcriptional analysis of three RNA polymerase sigma factor genes of Streptomyces coelicolor A3(2)

1990 • 155 citations

The polyketide metabolites

1992 • 117 citations

Purification and characterization of the acyl carrier protein of the Streptomyces glaucescens tetracenomycin C polyketide synthase

1992 • 96 citations

Flavanone Synthase from Petroselinum hortense

1979 • 95 citations

Three‐dimensional structure of acyl carrier protein in solution determined by nuclear magnetic resonance and the combined use of dynamical simulated annealing and distance geometry

1988 • 89 citations

Phage-mediated cloning of bldA, a region involved in Streptomyces coelicolor morphological development, and its analysis by genetic complementation

1985 • 88 citations

[21] Genetic manipulation of Streptomyces: Integrating vectors and gene replacement

1991 • 88 citations

Visualizing Gene Expression in Time and Space in the Filamentous Bacterium Streptomyces coelicolor

1988 • 85 citations

Polyketide synthase com plexes: their structure and function in antibiotic biosynthesis

1991 • 82 citations

Biosynthesis of the antibiotic actinorhodin. Analysis of blocked mutants of Streptomyces coelicolor.

1987 • 79 citations

Mutactin, a novel polyketide from Streptomyces coelicolor. Structure and biosynthetic relationship to actinorhodin

1990 • 63 citations

Overproduction and localization of components of the polyketide synthase of Streptomyces glaucescens involved in the production of the antibiotic tetracenomycin C

1991 • 58 citations

Cloning, characterization, and high-level expression in Escherichia coli of the Saccharopolyspora erythraea gene encoding an acyl carrier protein potentially involved in fatty acid biosynthesis

1991 • 54 citations

Genes for Polyketide Secondary Metabolic Pathways in Microorganisms and Plants

2007 • 54 citations

Cloning of spiramycin biosynthetic genes and their use in constructing Streptomyces ambofaciens mutants defective in spiramycin biosynthesis

1990 • 53 citations

The use of a chromosome integration vector to map erythromycin resistance and production genes in Saccharopolyspora erythraea (Streptomyces erythraeus)

1988 • 47 citations

The glucose kinase gene of Strptomyces coelicolor and its use in selecting spontaneous deletions for desired regions of the genome

1987 • 37 citations

Replacement of Streptomyces hygroscopicus genomic segments with in vitro altered DNA sequences.

1988 • 37 citations

Biosynthesis of branched long-chain fatty acids from the related short-chain α-keto acid substrates by a cell-free system of Bacillus subtilis

1973 • 34 citations

A rapid method for the analysis of plasmid content and copy number in various Streptomycetes grown on agar plates

1990 • 13 citations