Hepatitis C virus-encoded NS2-3 protease: cleavage-site mutagenesis and requirements for bimolecular cleavage

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

1987 • 11,491 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

On the size of the active site in proteases. I. Papain

1967 • 5,150 citations

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

1985 • 2,456 citations

Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.

1986 • 2,118 citations

FLAVIVIRUS GENOME ORGANIZATION, EXPRESSION, AND REPLICATION

1990 • 1,903 citations

Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region

1993 • 1,401 citations

Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.

1990 • 1,208 citations

Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes

1989 • 1,004 citations

Structure and organization of the hepatitis C virus genome isolated from human carriers

1991 • 970 citations

Expression and identification of hepatitis C virus polyprotein cleavage products

1993 • 890 citations

Isolation of Monoclonal Antibodies Specific for Human c-myc Proto-Oncogene Product

1985 • 788 citations

Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis.

1991 • 636 citations

Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites

1993 • 601 citations

Full-length sequence of a hepatitis C virus genome having poor homology to reported isolates: Comparative study of four distinct genotypes

1992 • 578 citations

Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins

1991 • 557 citations

Neutral endopeptidase 24.11 (enkephalinase) and related regulators of peptide hormones ¹

1989 • 552 citations

New mammalian expression vectors

1990 • 525 citations

Nucleotide sequence of the genomic RNA of hepatitis C virus isolated from a human carrier: comparison with reported isolates for conserved and divergent regions

1991 • 476 citations

Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus

1993 • 476 citations

Nonstructural protein 3 of the hepatitis C virus encodes a serine-type proteinase required for cleavage at the NS3/4 and NS4/5 junctions

1993 • 464 citations

NS3 is a serine protease required for processing of hepatitis C virus polyprotein

1993 • 454 citations

Classification and nomenclature of viruses. Fifth report of the International Committee on Taxonomy of Viruses.

1991 • 453 citations

A second hepatitis C virus-encoded proteinase.

1993 • 416 citations

Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins

1994 • 411 citations

Processing in the hepatitis C virus E2-NS2 region: identification of p7 and two distinct E2-specific products with different C termini

1994 • 347 citations

Proteolytic processing and membrane association of putative nonstructural proteins of hepatitis C virus.

1993 • 342 citations

Evidence that the N-terminal domain of nonstructural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyprotein.

1990 • 342 citations

Kinetic and structural analyses of hepatitis C virus polyprotein processing

1994 • 331 citations

Hepatitis C virus NS3 protein polynucleotide-stimulated nucleoside triphosphatase and comparison with the related pestivirus and flavivirus enzymes

1993 • 310 citations

Identification of Genotypes of Hepatitis C Virus by Sequence Comparisons in the Core, E1 and NS-5 Regions

1994 • 283 citations

Genomic structure of the human prototype strain H of hepatitis C virus: comparison with American and Japanese isolates.

1991 • 281 citations

Processing of the yellow fever virus nonstructural polyprotein: a catalytically active NS3 proteinase domain and NS2B are required for cleavages at dibasic sites

1991 • 242 citations

Deletion analysis of dengue virus type 4 nonstructural protein NS2B: identification of a domain required for NS2B-NS3 protease activity

1993 • 228 citations

A central region in the hepatitis C virus NS4A protein allows formation of an active NS3-NS4A serine proteinase complex in vivo and in vitro

1995 • 221 citations

In vitro processing of dengue virus type 2 nonstructural proteins NS2A, NS2B, and NS3

1990 • 212 citations

Hepatitis C virus NS3 serine proteinase: trans-cleavage requirements and processing kinetics

1994 • 206 citations

Comparative Specificity of Microbial Proteinases

1974 • 195 citations

Mutagenesis of the yellow fever virus NS2B protein: effects on proteolytic processing, NS2B-NS3 complex formation, and viral replication

1993 • 193 citations

Dengue 2 Virus NS2B and NS3 Form a Stable Complex That Can Cleave NS3 within the Helicase Domain

1993 • 185 citations

The Hepatitis C Virus Encodes a Serine Protease Involved in Processing of the Putative Nonstructural Proteins from the Viral Polyprotein Precursor

1993 • 183 citations

Pestivirus gene expression: Protein p80 of bovine viral diarrhea virus is a proteinase involved in polyprotein processing

1991 • 166 citations

Two hepatitis C virus glycoprotein E2 products with different C termini

1994 • 152 citations

Association of Sindbis virion glycoproteins and their precursors

1982 • 133 citations

Complex Processing and Protein:Protein Interactions in the E2:NS2 Region of HCV

1994 • 133 citations

Production of Nonstructural Proteins of Hepatitis C Virus Requires a Putative Viral Protease Encoded by NS3

1994 • 128 citations

Endopeptidase 24.15 from rat testes. Isolation of the enzyme and its specificity toward synthetic and natural peptides, including enkephalin-containing peptides

1989 • 117 citations

Processing and localization of Dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5

1992 • 112 citations

Entire nucleotide sequence and characterization of a hepatitis C virus of genotype V/3a

1994 • 102 citations

The entire nucleotide sequence and classification of a hepatitis C virus isolate of a novel genotype from an Indonesian patient with chronic liver disease

1994 • 96 citations

The hepatitis C virus NS3 serine proteinase and NS4A cofactor: establishment of a cell-free trans-processing assay.

1995 • 87 citations

Analysis of N-terminal processing of hepatitis C virus nonstructural protein 2

1994 • 74 citations

Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector

1993 • 47 citations

The coding region for the 54-kDa protein of several pestiviruses lacks host insertions but reveals a “Zinc finger-like” domain

1990 • 43 citations

Open Lung Biopsy Provides a Higher and More Specific Diagnostic Yield Compared to Broncho-Alveolar Lavage in Immunocompromised Patients

1995 • 41 citations

Description of a procedure which allows isolation of viral nonstructural proteins from BHK vertebrate cells infected with the West Nile flavivirus in a state which allows their direct chemical characterization

1990 • 28 citations