Imaging in Systems Biology

Stochastic Gene Expression in a Single Cell

2002 • 5,397 citations

Comprehensive Identification of Cell Cycle–regulated Genes of the YeastSaccharomyces cerevisiaeby Microarray Hybridization

1998 • 4,811 citations

CellProfiler: image analysis software for identifying and quantifying cell phenotypes.

2006 • 4,776 citations

A synthetic oscillatory network of transcriptional regulators

2000 • 4,543 citations

Correlation between Protein and mRNA Abundance in Yeast

1999 • 3,800 citations

Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin

1997 • 3,163 citations

Far-Field Optical Nanoscopy

2007 • 2,876 citations

A guide to choosing fluorescent proteins

2005 • 2,755 citations

Being Digital

1995 • 2,725 citations

The Fluorescent Toolbox for Assessing Protein Location and Function

2006 • 2,702 citations

Transcriptional regulatory code of a eukaryotic genome

2004 • 2,174 citations

The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements

2006 • 2,130 citations

Comparing protein abundance and mRNA expression levels on a genomic scale.

2003 • 1,602 citations

Single-cell proteomic analysis of S. cerevisiae reveals the architecture of biological noise

2006 • 1,532 citations

Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation

2002 • 1,516 citations

Control of Stochasticity in Eukaryotic Gene Expression

2004 • 1,448 citations

Patterns of Auxin Transport and Gene Expression during Primordium Development Revealed by Live Imaging of the Arabidopsis Inflorescence Meristem

2005 • 1,132 citations

Gene Regulation at the Single-Cell Level

2005 • 1,116 citations

The Gene Ontology (GO) project in 2006

2005 • 971 citations

Circularly permuted green fluorescent proteins engineered to sense Ca ²⁺

2001 • 941 citations

Circular permutation and receptor insertion within green fluorescent proteins

1999 • 913 citations

Multicolor and Electron Microscopic Imaging of Connexin Trafficking

2002 • 908 citations

Noise in protein expression scales with natural protein abundance

2006 • 828 citations

Gene regulatory networks for development

2005 • 730 citations

An excitable gene regulatory circuit induces transient cellular differentiation

2006 • 729 citations

Evaluation of gene expression measurements from commercial microarray platforms

2003 • 716 citations

MOLECULAR GENETICS OF SPORULATION IN BACILLUS SUBTILIS

1996 • 628 citations

An auxin-driven polarized transport model for phyllotaxis

2006 • 598 citations

Counting Cytokinesis Proteins Globally and Locally in Fission Yeast

2005 • 580 citations

A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C

2003 • 536 citations

Regulated cell-to-cell variation in a cell-fate decision system

2005 • 446 citations

A Genetically Encoded Optical Probe of Membrane Voltage

1997 • 424 citations

High-throughput fluorescence microscopy for systems biology

2006 • 421 citations

Interactome: gateway into systems biology

2005 • 400 citations

Automated cell lineage tracing in Caenorhabditis elegans

2006 • 366 citations

Real-time lineage analysis reveals oriented cell divisions associated with morphogenesis at the shoot apex ofArabidopsis thaliana

2004 • 327 citations

Segmenting and tracking fluorescent cells in dynamic 3-D microscopy with coupled active surfaces

2005 • 315 citations

A hybrid 3D watershed algorithm incorporating gradient cues and object models for automatic segmentation of nuclei in confocal image stacks

2003 • 315 citations

Two‐photon molecular excitation provides intrinsic 3‐dimensional resolution for laser‐based microscopy and microphotochemistry

1994 • 231 citations

Single-cell quantification of molecules and rates using open-source microscope-based cytometry

2007 • 222 citations

Digitizing life at the level of the cell: high-performance laser-scanning microscopy and image analysis for in toto imaging of development

2003 • 205 citations

Detection of programmed cell death using fluorescence energy transfer

1998 • 205 citations

Three-chromophore FRET microscopy to analyze multiprotein interactions in living cells

2004 • 186 citations

The Stanford Microarray Database: implementation of new analysis tools and open source release of software

2006 • 175 citations

Microarray reality checks in the context of a complex disease

2004 • 175 citations

A Quantitative Approach to the Study of Cell Shapes and Interactions during Early Chordate Embryogenesis

2006 • 144 citations

Cell Dynamics During Somite Boundary Formation Revealed by Time-Lapse Analysis

2002 • 140 citations

A database for management of gene expression data in situ

2004 • 123 citations

Multi-parameter analysis of the kinetics of NF-κB signalling and transcription in single living cells

2002 • 109 citations

Evaluation of confocal microscopy system performance

2001 • 106 citations

Applications of High-Throughput RNA Interference Screens to Problems in Cell and Developmental Biology

2007 • 106 citations

Small‐Molecule Screening and Profiling by Using Automated Microscopy

2004 • 102 citations

Biophysical mechanisms for morphogenetic progressions at the shoot apex

1992 • 84 citations

An array of problems

2005 • 73 citations

Proteome chips for whole-organism assays

2006 • 68 citations

Comparative evaluation of linear and exponential amplification techniques for expression profiling at the single-cell level.

2006 • 62 citations

Biological microarray interpretation: The rules of engagement

2006 • 49 citations

Unraveling genomic regulatory networks in the simple chordate, Ciona intestinalis

2005 • 38 citations

A comparison of some quick and simple threshold selection methods for stained cells.

1988 • 34 citations

Time-Lapse Microscopy of Brain Development

2004 • 33 citations

Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture

2004 • 24 citations

Live imaging and morphometric analysis of embryonic development in the ascidianCiona intestinalis

2005 • 23 citations

Application of 2D and 3D DIAS to Motion Analysis of Live Cells in Transmission and Confocal Microscopy Imaging

2006 • 22 citations

Confocal quantification of cis-regulatory reporter gene expression in living sea urchin

2006 • 21 citations

Anatomical ontologies: names and places in biology.

2005 • 11 citations