Targeting a Complex Transcriptome: The Construction of the Mouse Full-Length cDNA Encyclopedia

Initial sequencing and comparative analysis of the mouse genome

2002 • 6,971 citations

A simple and very efficient method for generating cDNA libraries

1983 • 4,606 citations

Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project

1991 • 2,286 citations

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences

2002 • 1,749 citations

Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs

2002 • 1,612 citations

The DNA sequence of human chromosome 21

2000 • 1,406 citations

λ ZAP: a bacteriophage λ expression vector within vivoexcision properties

1988 • 1,394 citations

The DNA sequence of human chromosome 22

1999 • 1,167 citations

Stromal Effects on Mammary Gland Development and Breast Cancer

2002 • 902 citations

Large-Scale Transcriptional Activity in Chromosomes 21 and 22

2002 • 839 citations

Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence.

1995 • 804 citations

Functional annotation of a full-length mouse cDNA collection

2001 • 663 citations

Patterns of Variant Polyadenylation Signal Usage in Human Genes

2000 • 650 citations

Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides

1994 • 584 citations

Normalization and subtraction: two approaches to facilitate gene discovery.

1996 • 542 citations

Generation and analysis of 280,000 human expressed sequence tags.

1996 • 474 citations

Analysis of expressed sequence tags indicates 35,000 human genes

2000 • 367 citations

Estimate of human gene number provided by genome-wide analysis using Tetraodon nigroviridis DNA sequence

2000 • 324 citations

An anatomy of normal and malignant gene expression

2002 • 310 citations

High-Efficiency Full-Length cDNA Cloning by Biotinylated CAP Trapper

1996 • 303 citations

Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes

2000 • 283 citations

Construction and characterization of a full length-enriched and a 5′-end-enriched cDNA library

1997 • 258 citations

Identification and Characterization of the Potential Promoter Regions of 1031 Kinds of Human Genes

2001 • 256 citations

Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA

1998 • 235 citations

DNA Microarrays: A Molecular Cloning Manual

2003 • 212 citations

Delineating developmental and metabolic pathwaysin vivoby expression profiling using the RIKEN set of 18,816 full-length enriched mouse cDNA arrays

2001 • 206 citations

Oligo(dT) primer generates a high frequency of truncated cDNAs through internal poly(A) priming during reverse transcription

2002 • 193 citations

A Drosophilafull-length cDNA resource

2002 • 190 citations

Impact of Alternative Initiation, Splicing, and Termination on the Diversity of the mRNA Transcripts Encoded by the Mouse Transcriptome

2003 • 189 citations

[2] High-efficiency full-length cDNA cloning

1999 • 189 citations

Identification and Characterization of the Potential Promoter Regions of 1031 Kinds of Human Genes

2001 • 186 citations

Diverse transcriptional initiation revealed by fine, large‐scale mapping of mRNA start sites

2001 • 168 citations

The Mouse Gene Expression Database (GXD)

2001 • 159 citations

The contribution of 700,000 ORF sequence tags to the definition of the human transcriptome

2001 • 142 citations

3′-End cleavage and polyadenylation of mRNA precursors

1995 • 136 citations

Alternate Polyadenylation in Human mRNAs: A Large-Scale Analysis by EST Clustering

1998 • 129 citations

An encyclopedia of mouse genes

1999 • 120 citations

A premature-termination mutation in the Mus musculus cyclin-dependent kinase 3 gene

2001 • 99 citations

Molecular Analysis of Gene Expression in the Developing Pontocerebellar Projection System

2002 • 95 citations

High Efficiency Selection of Full-length cDNA by Improved Biotinylated Cap Trapper

1997 • 94 citations

Significance of rare mRNA sequences in liver

1977 • 77 citations

Balanced-Size and Long-Size Cloning of Full-Length, Cap-Trapped cDNAs into Vectors of the Novel λ-FLC Family Allows Enhanced Gene Discovery Rate and Functional Analysis

2001 • 76 citations

Multiple tissue-specific promoters control expression of the murine tartrate-resistant acid phosphatase gene

2003 • 64 citations

RIKEN Integrated Sequence Analysis (RISA) System---384-Format Sequencing Pipeline with 384 Multicapillary Sequencer

2000 • 62 citations

Characterization of Gene Expression in Mouse Blastocyst Using Single-Pass Sequencing of 3995 Clones

1998 • 48 citations

Cloning Full-Length, Cap-Trapper-Selected cDNAs by Using the Single-Strand Linker Ligation Method

2001 • 47 citations

Continued Discovery of Transcriptional Units Expressed in Cells of the Mouse Mononuclear Phagocyte Lineage

2003 • 46 citations

Comparative evaluation of 5′-end-sequence quality of clones in CAP trapper and other full-length-cDNA libraries

2001 • 41 citations

Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display

1999 • 41 citations

Screening poly(dA/dT) ⁻ cDNAs for gene identification

2000 • 38 citations

Simple and rapid preparation of plasmid template by a filtration method using microtiter filter plates

1997 • 34 citations

Automated Filtration-Based High-Throughput Plasmid Preparation System

1999 • 33 citations

Extra-Long First-Strand cDNA Synthesis

2002 • 30 citations

The Mammalian Protein–Protein Interaction Database and Its Viewing System That Is Linked to the Main FANTOM2 Viewer

2003 • 30 citations

Comprehensive Analysis of the Mouse Metabolome Based on the Transcriptome

2003 • 30 citations

A Computer-Based Method of Selecting Clones for a Full-Length cDNA Project: Simultaneous Collection of Negligibly Redundant and Variant cDNAs

2002 • 30 citations

Computer-Based Methods for the Mouse Full-Length cDNA Encyclopedia: Real-Time Sequence Clustering for Construction of a Nonredundant cDNA Library

2001 • 28 citations

Inferring Alternative Splicing Patterns in Mouse from a Full-Length cDNA Library and Microarray Data

2002 • 25 citations

Computer-Based Methods for the Mouse Full-Length cDNA Encyclopedia: Real-Time Sequence Clustering for Construction of a Nonredundant cDNA Library

2001 • 22 citations

A premature-termination mutation in the Mus musculus cyclin-dependent kinase 3 gene

2001 • 22 citations

cDNA detection and analysis

2001 • 21 citations

Untitled

2001 • 14 citations

Removal of PolyA Tails from Full-Length cDNA Libraries for High-Efficiency Sequencing

2001 • 13 citations

Cytoplasmic RNA Extraction from Fresh and Frozen Mammalian Tissues

2002 • 13 citations