Computer-Based Methods for the Mouse Full-Length cDNA Encyclopedia: Real-Time Sequence Clustering for Construction of a Nonredundant cDNA Library

Improved tools for biological sequence comparison.

1988 • 11,455 citations

A general method applicable to the search for similarities in the amino acid sequence of two proteins

1970 • 11,049 citations

CAP3: A DNA Sequence Assembly Program

1999 • 5,180 citations

Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project

1991 • 2,286 citations

A gene map of the human genome.

1996 • 986 citations

Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence.

1995 • 804 citations

Sequence identification of 2,375 human brain genes

1992 • 764 citations

A Gene Map of the Human Genome

1996 • 737 citations

Frequent Alternative Splicing of Human Genes

1999 • 553 citations

Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression

1992 • 528 citations

Alternative poly(A) site selection in complex transcription units: means to an end?

1997 • 508 citations

TIGR Assembler: A New Tool for Assembling Large Shotgun Sequencing Projects

1995 • 413 citations

ESTablishing a human transcript map

1995 • 391 citations

THE BIOCHEMISTRY OF 3′-END CLEAVAGE AND POLYADENYLATION OF MESSENGER RNA PRECURSORS

1992 • 387 citations

Pieces of the puzzle: expressed sequence tags and the catalog of human genes

1997 • 380 citations

High-Efficiency Full-Length cDNA Cloning by Biotinylated CAP Trapper

1996 • 303 citations

Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes

2000 • 283 citations

Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA

1998 • 235 citations

High‐efficiency cloning of Arabidopsis full‐length cDNA by biotinylated CAP trapper

1998 • 218 citations

d2_cluster: A Validated Method for Clustering EST and Full-Length cDNA Sequences

1999 • 202 citations

A Comprehensive Approach to Clustering of Expressed Human Gene Sequence: The Sequence Tag Alignment and Consensus Knowledge Base

1999 • 201 citations

[2] High-efficiency full-length cDNA cloning

1999 • 189 citations

Single pass sequencing and physical and genetic mapping of human brain cDNAs

1992 • 173 citations

Assessing protein coding region integrity in cDNA sequencing projects.

1998 • 163 citations

3′-End cleavage and polyadenylation of mRNA precursors

1995 • 136 citations

Alternate Polyadenylation in Human mRNAs: A Large-Scale Analysis by EST Clustering

1998 • 129 citations

High Efficiency Selection of Full-length cDNA by Improved Biotinylated Cap Trapper

1997 • 94 citations

Expressed sequence tags and chromosomal localization of cDNA clones from a subtracted retinal pigment epithelium library

1992 • 64 citations

Characterization of Gene Expression in Mouse Blastocyst Using Single-Pass Sequencing of 3995 Clones

1998 • 48 citations

Comparative evaluation of 5′-end-sequence quality of clones in CAP trapper and other full-length-cDNA libraries

2001 • 41 citations

Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display

1999 • 41 citations

Transcriptional sequencing: A method for DNA sequencing using RNA polymerase

1998 • 38 citations

Automated Filtration-Based High-Throughput Plasmid Preparation System

1999 • 33 citations