Interactions between Centromere Complexes inSaccharomyces cerevisiae

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Mass spectrometry-based proteomics

2003 • 6,616 citations

Functional organization of the yeast proteome by systematic analysis of protein complexes

2002 • 4,751 citations

[56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe

1991 • 3,691 citations

Getting started with yeast

2002 • 3,496 citations

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

1985 • 2,456 citations

[1] Getting started with yeast

1991 • 2,456 citations

Heterologous modules for efficient and versatile PCR-based gene targeting inSchizosaccharomyces pombe

1998 • 2,230 citations

Sequencing and comparison of yeast species to identify genes and regulatory elements

2003 • 1,785 citations

Multifunctional yeast high-copy-number shuttle vectors

1992 • 1,656 citations

Cohesins: Chromosomal Proteins that Prevent Premature Separation of Sister Chromatids

1997 • 1,466 citations

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1987 • 1,375 citations

[19] Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast

1991 • 1,369 citations

Feedback control of mitosis in budding yeast

1991 • 1,167 citations

Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.

1988 • 983 citations

Centromeres and Kinetochores

2003 • 967 citations

Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.

1982 • 923 citations

Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae.

1984 • 838 citations

Finding Functional Features in Saccharomyces Genomes by Phylogenetic Footprinting

2003 • 828 citations

Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces.

1984 • 827 citations

Isolation of Monoclonal Antibodies Specific for Human c-myc Proto-Oncogene Product

1985 • 788 citations

Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p.

1996 • 777 citations

A Central Role for Cohesins in Sister Chromatid Cohesion, Formation of Axial Elements, and Recombination during Yeast Meiosis

1999 • 722 citations

HeterologousHIS3 Marker and GFP Reporter Modules for PCR-Targeting inSaccharomyces cerevisiae

1997 • 683 citations

Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies

1989 • 650 citations

Predicting coiled coils by use of pairwise residue correlations.

1995 • 626 citations

Phospho-Regulation of Kinetochore-Microtubule Attachments by the Aurora Kinase Ipl1p

2002 • 612 citations

GFP tagging of budding yeast chromosomes reveals that protein–protein interactions can mediate sister chromatid cohesion

1996 • 589 citations

Mutations that suppress the thermosensitivity of green fluorescent protein

1996 • 529 citations

Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast.

1993 • 506 citations

[14] Genetic applications of yeast transformation with linear and gapped plasmids

1983 • 503 citations

Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins.

1993 • 492 citations

A rapid method for localized mutagenesis of yeast genes

1992 • 456 citations

Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2

2002 • 426 citations

Evidence that the MIF2 gene of Saccharomyces cerevisiae encodes a centromere protein with homology to the mammalian centromere protein CENP-C.

1995 • 410 citations

The product of the spindle formation gene sad1+ associates with the fission yeast spindle pole body and is essential for viability.

1995 • 406 citations

Generation of protein-reactive antibodies by short peptides is an event of high frequency: implications for the structural basis of immune recognition.

1983 • 378 citations

Cse4p Is a Component of the Core Centromere of Saccharomyces cerevisiae

1998 • 365 citations

Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae.

1990 • 333 citations

Cohesin ensures bipolar attachment of microtubules to sister centromeres and resists their precocious separation

2000 • 330 citations

Purification of a RAS-Responsive Adenylyl Cyclase Complex from Saccharomyces cerevisiae by Use of an Epitope Addition Method

1988 • 328 citations

Three-dimensional reconstruction and analysis of mitotic spindles from the yeast, Schizosaccharomyces pombe.

1993 • 320 citations

Analysis of the Saccharomyces Spindle Pole by Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry

1998 • 318 citations

The Ndc80p Complex from Saccharomyces cerevisiae Contains Conserved Centromere Components and Has a Function in Chromosome Segregation

2001 • 318 citations

A 240 kd multisubunit protein complex, CBF3, is a major component of the budding yeast centromere

1991 • 313 citations

High-Voltage Electron Tomography of Spindle Pole Bodies and Early Mitotic Spindles in the YeastSaccharomyces cerevisiae

1999 • 283 citations

hNuf2 inhibition blocks stable kinetochore–microtubule attachment and induces mitotic cell death in HeLa cells

2002 • 281 citations

Electron-microscopic study of the spindle and chromosome movement in the yeast Saccharomyces Cerevisiae

1976 • 280 citations

Establishing Biorientation Occurs with Precocious Separation of the Sister Kinetochores, but Not the Arms, in the Early Spindle of Budding Yeast

2000 • 280 citations

Determining centromere identity: cyclical stories and forking paths

2001 • 280 citations

Components of the yeast spindle and spindle pole body.

1990 • 269 citations

Transient Sister Chromatid Separation and Elastic Deformation of Chromosomes during Mitosis in Budding Yeast

2000 • 269 citations

NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae.

1993 • 264 citations

The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity

2003 • 254 citations

Centromere clustering is a major determinant of yeast interphase nuclear organization

2000 • 245 citations

Spc98p and Spc97p of the yeast gamma -tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p

1997 • 239 citations

Molecular Analysis of Kinetochore-Microtubule Attachment in Budding Yeast

2001 • 236 citations

Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway

2003 • 233 citations

Pds1p is required for faithful execution of anaphase in the yeast, Saccharomyces cerevisiae

1996 • 219 citations

The identification of a second cell cycle control on the HO promoter in yeast: Cell cycle regulation of SWI5 nuclear entry

1990 • 215 citations

Kinetochore microtubules in PTK cells.

1992 • 213 citations

Budding Yeast Chromosome Structure and Dynamics during Mitosis

2001 • 210 citations

Localization of Core Spindle Pole Body (SPB) Components during SPB Duplication in Saccharomyces cerevisiae

1999 • 207 citations

A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore

1999 • 197 citations

Purification of NSP1 reveals complex formation with ‘GLFG’ nucleoporins and a novel nuclear pore protein NIC96.

1993 • 189 citations

The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control

2001 • 185 citations

Implication of a novel multiprotein Dam1p complex in outer kinetochore function

2001 • 175 citations

Budding yeast centromere composition and assembly as revealed by in vivo cross-linking

1997 • 173 citations

The mitotic spindle is required for loading of the DASH complex onto the kinetochore

2002 • 170 citations

Stu2p: A Microtubule-Binding Protein that Is an Essential Component of the Yeast Spindle Pole Body

1997 • 164 citations

Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation

2002 • 161 citations

Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells

2003 • 158 citations

Fission Yeast Bub1 Is a Mitotic Centromere Protein Essential for the Spindle Checkpoint and the Preservation of Correct Ploidy through Mitosis

1998 • 156 citations

A computationally directed screen identifying interacting coiled coils from Saccharomyces cerevisiae

2000 • 155 citations

NDC1: a nuclear periphery component required for yeast spindle pole body duplication

1993 • 151 citations

The N Terminus of the Centromere H3-Like Protein Cse4p Performs an Essential Function Distinct from That of the Histone Fold Domain

2000 • 147 citations

Mitotic Spindle Integrity and Kinetochore Function Linked by the Duo1p/Dam1p Complex

2001 • 147 citations

Slk19p Is a Centromere Protein That Functions to Stabilize Mitotic Spindles

1999 • 142 citations

HEC, a Novel Nuclear Protein Rich in Leucine Heptad Repeats Specifically Involved in Mitosis

1997 • 137 citations

Checkpoint Genes Required To Delay Cell Division in Response to Nocodazole Respond to Impaired Kinetochore Function in the Yeast Saccharomyces cerevisiae

1995 • 137 citations

Simple centromere, complex kinetochore

2002 • 134 citations

The fission yeast SPB component Cut12 links bipolar spindle formation to mitotic control

1998 • 131 citations

A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint

2001 • 126 citations

Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore

2002 • 121 citations

Kinetochore Protein Interactions and their Regulation by the Aurora Kinase Ipl1p

2003 • 120 citations

Evolutionary conservation between budding yeast and human kinetochores

2001 • 113 citations

Captivating Capture: How Microtubules Attach to Kinetochores

2003 • 108 citations

Ctf19p: A Novel Kinetochore Protein in Saccharomyces cerevisiae and a Potential Link between the Kinetochore and Mitotic Spindle

1999 • 106 citations

Polyploids require Bik1 for kinetochore–microtubule attachment

2001 • 104 citations

Flow cytometric analysis of DNA content in budding yeast

1997 • 96 citations

Cloning and analysis of the Kluyveromyces lactis TRP1 gene: A chromosomal locus flanked by genes encoding inorganic pyrophosphatase and histone H3

1989 • 94 citations

Purification of a yeast centromere-binding protein that is able to distinguish single base-pair mutations in its recognition site.

1989 • 81 citations

Fluorescence resonance energy transfer using color variants of green fluorescent protein

2002 • 77 citations

Nuf2, a spindle pole body-associated protein required for nuclear division in yeast.

1994 • 74 citations

Meiotic behaviours of chromosomes and microtubules in budding yeast: relocalization of centromeres and telomeres during meiotic prophase

1998 • 74 citations

Requirement of Chromatid Cohesion Proteins Rad21/Scc1 and Mis4/Scc2 for Normal Spindle-Kinetochore Interaction in Fission Yeast

2002 • 67 citations

Chl4p and Iml3p Are Two New Members of the Budding Yeast Outer Kinetochore

2003 • 64 citations

Dad1p, Third Component of the Duo1p/Dam1p Complex Involved in Kinetochore Function and Mitotic Spindle Integrity

2001 • 62 citations

Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a New Group of Proteins Important for Chromosome Segregation in Saccharomyces cerevisiae

2002 • 50 citations

Purification of a protein binding to the CDEI subregion of Saccharomyces cerevisiae centromere DNA.

1989 • 45 citations

Behavior of kinetochores during mitosis in the fungus Saprolegnia ferax.

1980 • 40 citations

Purification of a Yeast Centromere-Binding ProteinThat Is Able To Distinguish Single Base-Pair Mutations in Its Recognition Site

1989 • 37 citations

Purification of a Protein Binding to the CDEI Subregion of Saccharomyces cerevisiae Centromere DNA

1989 • 23 citations

Meiotic cytology of Saccharomyces cerevisiae in protoplast lysates

1982 • 23 citations

NNF1 is an essential yeast gene required for proper spindle orientation, nucleolar and nuclear envelope structure and mRNA export

1997 • 15 citations