Engineering streptococcal protein G for increased alkaline stability

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Calculation of protein extinction coefficients from amino acid sequence data

1989 • 5,198 citations

RASMOL: biomolecular graphics for all

1995 • 2,601 citations

A general method ofin vitropreparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

1988 • 2,410 citations

Protein secondary structure and circular dichroism: A practical guide

1990 • 926 citations

Encyclopedia of bioprocess technology: fermentation, biocatalysis, and bioseparation

1999 • 872 citations

A Novel, Highly Stable Fold of the Immunoglobulin Binding Domain of Streptococcal Protein G

1991 • 794 citations

Molecular clocks

2001 • 495 citations

Nonenzymatic Deamidation of Asparaginyl and Glutaminyl Residues in Protein

1991 • 411 citations

Two Crystal Structures of the B1 Immunoglobulin-Binding Domain of Streptococcal Protein G and Comparison with NMR

1994 • 409 citations

α-Helix stability in proteins

1992 • 361 citations

Crystal structure of the C2 fragment of streptococcal protein G in complex with the Fc domain of human IgG

1995 • 355 citations

The Third IgG-Binding Domain from Streptococcal Protein G

1994 • 340 citations

Design, synthesis, and application of a Protein A mimetic

1998 • 289 citations

Thermodynamic analysis of the folding of the streptococcal protein G IgG-binding domains B1 and B2: why small proteins tend to have high denaturation temperatures

1992 • 250 citations

Improving the Packing and Accuracy of NMR Structures with a Pseudopotential for the Radius of Gyration

1999 • 235 citations

Tertiary Structure Is a Principal Determinant to Protein Deamidation

1988 • 209 citations

Crystal structure of a streptococcal protein G domain bound to an Fab fragment

1992 • 180 citations

High-level production of uniformly 15N-and 13C-enriched fusion proteins in Escherichia coli

1996 • 179 citations

Analysis and use of the serum albumin binding domains of streptococcal protein G

1988 • 161 citations

Kinetic analysis of folding and unfolding the 56 amino acid IgG-binding domain of streptococcal protein G

1992 • 158 citations

Helix Propensities Are Identical in Proteins and Peptides

1997 • 149 citations

Fusion tails for the recovery and purification of recombinant proteins

1991 • 146 citations

Secondary Structure and Protein Deamidation

1999 • 136 citations

Structure and evolution of the repetitive gene encoding streptococcal protein G

1987 • 135 citations

Protein a mimetic peptide ligand for affinity purification of antibodies

1996 • 129 citations

Distribution of glutamine and asparagine residues and their near neighbors in peptides and proteins.

1991 • 128 citations

Effect of protein conformation on rate of deamidation: Ribonuclease A

1989 • 102 citations

1.67-.ANG. X-ray structure of the B2 immunoglobulin-binding domain of streptococcal protein G and comparison to the NMR structure of the B1 domain

1992 • 97 citations

Role of peptide conformation in the rate and mechanism of deamidation of asparaginyl residues

1988 • 91 citations

Protein engineering of an IgG-binding domain allows milder elution conditions during affinity chromatography

2000 • 85 citations

The effects of alpha‐helix on the stability of Asn residues: Deamidation rates in peptides of varying helicity

1999 • 80 citations

The serum albumin‐binding domain of streptococcal protein G is a three‐helical bundle: a heteronuclear NMR study

1996 • 74 citations

Analysis of rpsD mutations in Escherichia coli. I:

1979 • 66 citations

Determination of the solution structures of domains II and III of protein G from Streptococcus by 1H nuclear magnetic resonance

1992 • 62 citations

Dissection of the protein G B1 domain binding site for human IgG Fc fragment

1999 • 50 citations

Charge engineering of a protein domain to allow efficient ion-exchange recovery

2000 • 46 citations

Stability towards alkaline conditions can be engineered into a protein ligand

2000 • 46 citations

Thermodynamic genetics of the folding of the B1 immunoglobulin‐binding domain from streptococcal protein G

1995 • 37 citations

Reactivity toward deamidation of asparagine residues in β‐turn structures

2000 • 32 citations

Development of a cleaning in place protocol and repetitive application of Escherichia coli homogenate on STREAMLINE™ Q XL

2000 • 25 citations

The secondary structure of protein G′, a robust molecule

1991 • 20 citations

Stabilization of lysozyme against irreversible inactivation by alterations of the Asp-Gly sequences

1995 • 17 citations