Deamidation of asparagine and glutamine residues in proteins and peptides: structural determinants and analytical methodology

Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides. Succinimide-linked reactions that contribute to protein degradation.

1987 • 1,275 citations

The Structure of a Complex of Recombinant Hirudin and Human α-Thrombin

1990 • 718 citations

On-line mass spectrometric detection for capillary zone electrophoresis

1987 • 619 citations

Succinimide Formation from Aspartyl and Asparaginyl Peptides as a Model for the Spontaneous Degradation of Proteins

1989 • 504 citations

Adaptation of the equipment for high-performance electrophoresis to isoelectric focusing

1985 • 430 citations

Capillary zone electrophoresis-mass spectrometry using an electrospray ionization interface

1988 • 386 citations

Refined structure of the Hirudin-thrombin complex

1991 • 370 citations

Crystal structure of the thrombin-hirudin complex: a novel mode of serine protease inhibition.

1990 • 362 citations

Effects of amino acid sequence, buffers, and ionic strength on the rate and mechanism of deamidation of asparagine residues in small peptides.

1991 • 351 citations

PROTEIN CARBOXYL METHYLTRANSFERASES: TWO DISTINCT CLASSES OF ENZYMES

1985 • 311 citations

The Amino‐Acid Sequence of the αA₂ Chain of Bovine α‐Crystallin

1973 • 304 citations

Integration of mass spectrometry in analytical biotechnology

1991 • 242 citations

Tertiary Structure Is a Principal Determinant to Protein Deamidation

1988 • 209 citations

Stoichiometric methylation of porcine adrenocorticotropin by protein carboxyl methyltransferase requires deamidation of asparagine 25. Evidence for methylation at the alpha-carboxyl group of atypical L-isoaspartyl residues.

1984 • 202 citations

Synthetic peptide substrates for the erythrocyte protein carboxyl methyltransferase. Detection of a new site of methylation at isomerized L-aspartyl residues.

1984 • 201 citations

Nonenzymatic Covalent Posttranslational Modification of Proteins In Vivo

1985 • 197 citations

The Amino‐Acid Sequence of the αB₂ Chain of Bovine α‐Crystallin

1974 • 182 citations

Capillary isoelectric focusing of proteins in uncoated fused silica capillaries using polymeric additives

1991 • 164 citations

Sequence and structure determinants of the nonenzymatic deamidation of asparagine and glutamine residues in proteins

1991 • 163 citations

Solution structure of recombinant hirudin and the Lys-47 .fwdarw. Glu mutants: a nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing study

1989 • 155 citations

Formation of Isoaspartate at Two Distinct Sites during in vitro Aging of Human Growth Hormone

1989 • 145 citations

Rates of nonenzymic deamidation of glutaminyl and asparaginyl residues in pentapeptides

1973 • 137 citations

Amino-Acid Sequence of Horse Heart Cytochrome C : Peptides released by Digestion with Chymotrypsin

1961 • 129 citations

Distribution of glutamine and asparagine residues and their near neighbors in peptides and proteins.

1991 • 128 citations

Conformation of recombinant desulfatohirudin in aqueous solution determined by nuclear magnetic resonance

1989 • 126 citations

Deamidation of the asparaginyl‐glycyl sequence

1986 • 123 citations

Characterization of the tryptic map of recombinant DNA derived tissue plasminogen activator by high-performance liquid chromatography-electrospray ionization mass spectrometry

1991 • 120 citations

Tuna cytochrome c at 2.0 A resolution. II. Ferrocytochrome structure analysis.

1977 • 120 citations

Capillary electrophoresis—mass spectrometry

1993 • 116 citations

Multiple Forms of Cytochrome c in the Rat

1968 • 116 citations

Role of peptide conformation in the rate and mechanism of deamidation of asparaginyl residues

1988 • 91 citations

The unusual substrate specificity of eukaryotic protein carboxyl methyltransferases

1987 • 89 citations

Primary structures of the α‐crystallin A chains of twenty‐eight mammalian species, chicken and frog

1984 • 77 citations

Deamidation: a source of microheterogeneity in pharmaceutical proteins

1992 • 69 citations

Isolation and characterization of a succinimide variant of methionyl human growth hormone

1991 • 65 citations

Manipulation of electroosmotic flow in capillary electophoresis

1993 • 65 citations

Analysis of proteins and glycoproteins at the picomole level by on-line coupling of microbore high-performance liquid chromatography with flow fast atom bombardment and electrospray mass spectrometry: A comparative evaluation

1990 • 58 citations

Age‐dependent deamidation of αB‐crystallin

1993 • 51 citations

Sequence-specific deamidation: isolation and biochemical characterization of succinimide intermediates of recombinant hirudin

1993 • 51 citations

On the Heterogeneity of Beef Heart Cytochrome c. I. Separation and Isolation of Subfractions by Disc Electrophoresis and Column Chromatography.

1964 • 48 citations

Improvements in the method developed for performing isoelectric focusing in uncoated capillaries

1992 • 45 citations

Formation of isoaspartate 99 in bovine and porcine somatotropins

1990 • 41 citations

SEQUENCE DEPENDENT DEAMIDATION RATES FOR MODEL PEPTIDES OF CYTOCHROME C

1974 • 39 citations

Rugged method for the determination of deamidation products in insulin solutions by free zone capillary electrophoresis using an untreated fused-silica capillary

1992 • 33 citations

β-Aspartylglycine in urine and enzymic hydrolyzates of proteins

1966 • 31 citations

Characterization of the deamidated forms of recombinant hirudin

1992 • 27 citations

Capillary electrophoresis-electrospray mass spectrometry for the analysis of recombinant bovine and porcine somatotropins

1992 • 27 citations

Quantitative Studies of Urinary β-Aspartyl Oligopeptides^*

1966 • 26 citations

Solid‐state conformations of aminosuccinyl peptides: Crystal structure of tert‐butyloxycarbonyl‐<scp>L</scp>‐leucyl‐<scp>L</scp>‐aminosuccinyl‐<scp>L</scp>‐phenylalaninamide

1989 • 21 citations

Aspartimide formation in the joining peptide sequence of porcine and mouse pro-opiomelanocortin.

1993 • 20 citations

Analysis of recombinant proteins by isoelectric focusing in immobilized pH gradients

1992 • 13 citations