Mutations in Hepatitis C Virus RNAs Conferring Cell Culture Adaptation

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Replication of Subgenomic Hepatitis C Virus RNAs in a Hepatoma Cell Line

1999 • 2,796 citations

Virus taxonomy: classification and nomenclature of viruses. Seventh report of the International Committee on Taxonomy of Viruses.

2000 • 2,555 citations

Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.

1982 • 1,367 citations

Mutations in the Nonstructural Protein 5a Gene and Response to Interferon in Patients with Chronic Hepatitis C Virus 1b Infection

1996 • 1,016 citations

Internal ribosome entry site within hepatitis C virus RNA

1992 • 914 citations

Expression and identification of hepatitis C virus polyprotein cleavage products

1993 • 890 citations

Evidence That Hepatitis C Virus Resistance to Interferon Is Mediated through Repression of the PKR Protein Kinase by the Nonstructural 5A Protein

1997 • 824 citations

Transmission of Hepatitis C by Intrahepatic Inoculation with Transcribed RNA

1997 • 718 citations

Identification and properties of the RNA-dependent RNA polymerase of hepatitis C virus.

1996 • 694 citations

Comparison of full-length sequences of interferon-sensitive and resistant hepatitis C virus 1b. Sensitivity to interferon is conferred by amino acid substitutions in the NS5A region.

1995 • 638 citations

Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis.

1991 • 636 citations

Replication of hepatitis C virus

2000 • 625 citations

Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: the crystal structure provides insights into the mode of unwinding

1998 • 613 citations

Control of PKR Protein Kinase by Hepatitis C Virus Nonstructural 5A Protein: Molecular Mechanisms of Kinase Regulation

1998 • 603 citations

Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites

1993 • 601 citations

Hepatitis C Virus-Encoded Enzymatic Activities and Conserved RNA Elements in the 3′ Nontranslated Region Are Essential for Virus Replication In Vivo

2000 • 594 citations

Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus

1999 • 564 citations

The International Committee on Taxonomy of Viruses

1995 • 526 citations

Transcripts from a single full-length cDNA clone of hepatitis C virus are infectious when directly transfected into the liver of a chimpanzee

1997 • 525 citations

Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity

1997 • 518 citations

Overview of Hepatitis C Virus Genome Structure, Polyprotein Processing, and Protein Properties

2000 • 505 citations

Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus

1993 • 476 citations

Electroporation in ‘intracellular’ buffer increases cell survival

1992 • 460 citations

NS3 is a serine protease required for processing of hepatitis C virus polyprotein

1993 • 454 citations

Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA

1996 • 450 citations

Translation of human hepatitis C virus RNA in cultured cells is mediated by an internal ribosome-binding mechanism

1993 • 435 citations

A second hepatitis C virus-encoded proteinase.

1993 • 416 citations

Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins

1994 • 411 citations

Molecular views of viral polyprotein processing revealed by the crystal structure of the hepatitis C virus bifunctional protease–helicase

1999 • 378 citations

Infectious Transcripts and cDNA Clones of RNA Viruses

1994 • 340 citations

Kinetic and structural analyses of hepatitis C virus polyprotein processing

1994 • 331 citations

Hepatitis C virus NS3 protein polynucleotide-stimulated nucleoside triphosphatase and comparison with the related pestivirus and flavivirus enzymes

1993 • 310 citations

C-Terminal Domain of the Hepatitis C Virus NS3 Protein Contains an RNA Helicase Activity

1995 • 299 citations

Structure of the 3' terminus of the hepatitis C virus genome

1996 • 286 citations

A Novel Sequence Found at the 3′-Terminus of Hepatitis C Virus Genome

1995 • 283 citations

Phosphorylation of hepatitis C virus-encoded nonstructural protein NS5A

1995 • 281 citations

Structural Requirements for Initiation of Translation by Internal Ribosome Entry within Genome-Length Hepatitis C Virus RNA

1996 • 253 citations

Hepatitis C virus-encoded nonstructural protein NS4A has versatile functions in viral protein processing

1995 • 247 citations

RNA-dependent RNA Polymerase Activity of the Soluble Recombinant Hepatitis C Virus NS5B Protein Truncated at the C-terminal Region

1998 • 243 citations

In vivo analysis of the 3′ untranslated region of the hepatitis C virus after in vitro mutagenesis of an infectious cDNA clone

1999 • 220 citations

Production of Two Phosphoproteins from the NS5A Region of the Hepatitis C Viral Genome

1994 • 217 citations

Genetic Analysis of Internal Ribosomal Entry Site on Hepatitis C Virus RNA: Implication for Involvement of the Highly Ordered Structure and Cell Type-Specific Transacting Factors

1997 • 209 citations

Hepatitis C virus NS3 serine proteinase: trans-cleavage requirements and processing kinetics

1994 • 206 citations

Secondary structure determination of the conserved 98-base sequence at the 3' terminus of hepatitis C virus genome RNA

1997 • 193 citations

Determination of the secondary structure of and cellular protein binding to the 3'-untranslated region of the hepatitis C virus RNA genome

1997 • 177 citations

Phosphorylation of the hepatitis C virus NS5A protein in vitro and in vivo: properties of the NS5A-associated kinase

1997 • 177 citations

Selection of RNA Replicons Capable of Persistent Noncytopathic Replication in Mammalian Cells

1999 • 175 citations

Almost the entire 5′ non‐translated region of hepatitis C virus is required for cap‐independent translation

1995 • 159 citations

Infectious Bovine Viral Diarrhea Virus (Strain NADL) RNA from Stable cDNA Clones: a Cellular Insert Determines NS3 Production and Viral Cytopathogenicity

1998 • 149 citations

An infectious molecular clone of a Japanese genotype 1b hepatitis C virus

1999 • 143 citations

Genetic Organization and Diversity of the 3′ Noncoding Region of the Hepatitis C Virus Genome

1996 • 126 citations

Complete nucleotide sequence of a cell culture-adapted variant of hepatitis a virus: Comparison with wild-type virus with restricted capacity for in vitro replication

1988 • 118 citations

Cell death induced by cytopathic bovine viral diarrhoea virus is mediated by apoptosis

1996 • 115 citations

The N-terminal region of hepatitis C virus-encoded NS5A is important for NS4A-dependent phosphorylation

1997 • 115 citations

Electroporation efficiency in mammalian cells is increased by dimethyl sulfoxide (DMSO)

1996 • 109 citations

Nucleotide sequence of wild-type hepatitis A virus GBM in comparison with two cell culture-adapted variants

1994 • 85 citations

Induction of apoptosis and cleavage of poly(ADP-ribose) polymerase by cytopathic bovine viral diarrhea virus infection

1997 • 72 citations

Mutations in both the 2B and 2C genes of hepatitis A virus are involved in adaptation to growth in cell culture

1992 • 72 citations

Attenuated hepatitis A virus: genetic determinants of adaptation to growth in MRC-5 cells

1994 • 70 citations

Infection of a chimpanzee with hepatitis C virus grown in cell culture.

1998 • 54 citations

Genetic analysis of the hepatitis C virus (HCV) genome from HCV-infected human T cells.

1997 • 52 citations

Synergism of mutations in bacteriophage qβ RNA affecting host factor dependence of qβ replicase

2000 • 33 citations