Chemical modification of 3.alpha.,20.beta.-hydroxysteroid dehydrogenase with diethyl pyrocarbonate. Evidence for an essential, highly reactive, lysyl residue

Tissue sulfhydryl groups

1959 • 25,130 citations

[41] Modification of histidyl residues in proteins by diethylpyrocarbonate

1977 • 813 citations

Inactivation of Myosin by 2,4-Dinitrophenol and Protection by Adenosine Triphosphate and Other Phosphate Compounds

1963 • 409 citations

Ethoxyformylation of proteins. Reaction of ethoxyformic anhydride with .alpha.-chymotrypsin, pepsin, and pancreatic ribonuclease at pH 4

1970 • 388 citations

Evidence of an essential histidine residue in thermolysin

1974 • 210 citations

Pyruvate Carboxylase

1965 • 209 citations

Specific Chemical Modification of Proteins

1970 • 181 citations

Ionic properties of an essential histidine residue in pig heart lactate dehydrogenase

1973 • 176 citations

Biocatalysis in water-organic solvent two-phase systems

1984 • 168 citations

Enzymatic conversion of apolar compounds in organic media using an NADH‐regenerating system and dihydrogen as reductant

1983 • 123 citations

Bifunctional enzyme activity at the same active site: study of 3.alpha. and 20.beta. activity by affinity alkylation of 3.alpha.,20.beta.-hydroxysteroid dehydrogenase with 17-(bromoacetoxy)steroids

1980 • 77 citations

Effects of chemical modification on the activity of Crotalus adamanteus phospholipase A₂. Evidence for an essential amino group

1973 • 76 citations

Evidence for an essential histidine residue in S-adenosylhomocysteinase from rat liver

1983 • 66 citations

The Role of an Essential Histidine Residue of Yeast Alcohol Dehydrogenase

1975 • 59 citations

Involvement of histidine residues in the activity of horse liver alcohol dehydrogenase

1983 • 50 citations

Reaction mechanism and stereospecificity of 20 β-hydroxysteroid dehydrogenase

1968 • 43 citations

Modification of histidines in human prothrombin. Effect on the interaction of fibrinogen with thrombin from diethyl pyrocarbonate-modified prothrombin.

1985 • 31 citations

Comparison of the 3α- and 20β-hydroxysteroid dehydrogenase activities of the cortisone reductase of Streptomyces hydrogenans

1978 • 30 citations

The molecular weight and substrate specificity of 20 β-hydroxysteroid dehydrogenase from Streptomyces hydrogenans

1973 • 24 citations

Affinity labeling of steroid binding sites. Synthesis of 21-bromoacetylaminoprogesterone and study of 20beta-hydroxysteroid dehydrogenase.

1978 • 19 citations

Effect of the lyotropic series of anions on denaturation and renaturation of 20β-hydroxysteroid dehydrogenase

1984 • 17 citations

Nicotinamide adenine dinucleotide binding and promotion of enzyme activity: model based on affinity labeling of 3.alpha.,20.beta.-hydroxysteroid dehydrogenase with a nucleoside

1981 • 14 citations

Renaturation and urea-induced denaturation of 20β-hydroxysteroid dehydrogenase studied in solution and in the immobilized state

1980 • 11 citations