Key Residues in Subsite F Play a Critical Role in the Activity of Pseudomonas fluorescens Subspecies cellulosa Xylanase A Against Xylooligosaccharides but Not Against Highly Polymeric Substrates such as Xylan

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar

1959 • 26,991 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

1983 • 2,609 citations

New families in the classification of glycosyl hydrolases based on amino acid sequence similarities

1993 • 1,995 citations

[6] Quantitation of protein

1990 • 1,401 citations

Microbial xylanolytic systems

1985 • 954 citations

Bacterial cellulases and xylanases

1993 • 388 citations

Stereoselective hydrolysis catalyzed by related beta-1,4-glucanases and beta-1,4-xylanases.

1992 • 209 citations

Crystallographic observation of a covalent catalytic intermediate in a β-glycosidase

1996 • 163 citations

Mode of action and substrate specificities of cellulases from cloned bacterial genes.

1984 • 163 citations

A common protein fold and similar active site in two distinct families of β-glycanases

1995 • 155 citations

Crystal structure, at 2.6-A resolution, of the Streptomyces lividans xylanase A, a member of the F family of beta-1,4-D-glycanases.

1994 • 152 citations

Crystal structure of the catalytic domain of the .beta.-1,4-glycanase Cex from Cellulomonas fimi

1994 • 139 citations

The Acid/Base Catalyst in the Exoglucanase/Xylanase from Cellulomonas fimi Is Glutamic Acid 127: Evidence from Detailed Kinetic Studies of Mutants

1994 • 132 citations

Structure of the catalytic core of the family F xylanase from Pseudomonas fluorescens and identification of the xylopentaose-binding sites

1994 • 131 citations

Glutamic acid 274 is the nucleophile in the active site of a “retaining” exoglucanase from Cellulomonas fimi

1991 • 126 citations

Substrate-Binding Site of Endo-1,4-beta-Xylanase of the Yeast Cryptococcus albidus

1981 • 115 citations

Conserved serine‐rich sequences in xylanase and cellulase from Pseudomonas fluorescens subspecies cellulosa: internal signal sequence and unusual protein processing

1989 • 106 citations

Mechanisms of Cellulases and Xylanases: A Detailed Kinetic Study of the Exo-.beta.-1,4-glycanase from Cellulomonas Fimi

1994 • 102 citations

Structural and functional relationships in two families of β‐1,4‐glycanases

1991 • 92 citations

Novel cellulose-binding domains, NodB homologues and conserved modular architecture in xylanases from the aerobic soil bacteria Pseudomonas fluorescens subsp. cellulosa and Cellvibrio mixtus

1995 • 79 citations

A simple general synthesis of 2,4-dinitrophenyl glycopyranosides

1973 • 38 citations

Alteration of the cleavage mode and of the transglycosylation reactions of the xylanase A of Streptomyces lividans 1326 by site‐directed mutagenesis of the Asn173 residue

1994 • 38 citations

Identification of two acidic residues involved in the catalysis of xylanase A from Streptomyces lividans

1994 • 33 citations

Carbohydrate bioengineering

1996 • 15 citations

Crystallization and Preliminary X-ray Analysis of the Catalytic Domain of Xylanase A from Pseudomonas fluorescens subspecies cellulosa

1993 • 14 citations