Reconstitution of the degradation of the mRNA for ribosomal protein S20 with purified enzymes

Molecular cloning: A laboratory manual

1990 • 85,659 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

A DEAD-box RNA helicase in the Escherichia coli RNA degradosome

1996 • 547 citations

Copurification of E. coli RNAase E and PNPase: Evidence for a specific association between two enzymes important in RNA processing and degradation

1994 • 430 citations

Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12.

1986 • 427 citations

Proteins associated with RNase E in a multicomponent ribonucleolytic complex.

1996 • 349 citations

Mechanisms of mRNA decay in bacteria: a perspective

1988 • 290 citations

Polyadenylylation helps regulate mRNA decay in Escherichia coli.

1995 • 253 citations

A protein complex mediating mRNA degradation in Escherichia coli

1994 • 221 citations

The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli.

1991 • 219 citations

The first step in the functional inactivation of the Escherichia coli polynucleotide phosphorylase messenger is a ribonuclease III processing at the 5′ end.

1987 • 218 citations

RNase E, an endoribonuclease, has a general role in the chemical decay of Escherichia coli mRNA: evidence that rne and ams are the same genetic locus

1990 • 209 citations

RNA degradation in Escherichia coli regulated by 3' adenylation and 5' phosphorylation

1995 • 195 citations

Maturation of Escherichia coli tryptophan operon mRNA: evidence for 3′ exonucleolytic processing after rho-dependent termination.

1985 • 188 citations

RNase E, an RNA processing enzyme from Escherichia coli.

1979 • 178 citations

The Escherichia coli pcnB gene promotes adenylylation of antisense RNAI of ColE1-type plasmids in vivo and degradation of RNAI decay intermediates.

1993 • 162 citations

Enzymatic basis for hydrolytic versus phosphorolytic mRNA degradation in Escherichia coli and Bacillus subtilis.

1991 • 155 citations

mRNA degradation by processive 3′-5′ exoribonucleases in Vitro and the implications for prokaryotic mRNA decay in Vivo

1991 • 149 citations

Polyadenylylation destabilizes the rpsO mRNA of Escherichia coli.

1995 • 147 citations

Nucleotide sequence of the pnp gene of Escherichia coli encoding polynucleotide phosphorylase. Homology of the primary structure of the protein with the RNA-binding domain of ribosomal protein S1.

1987 • 135 citations

Polyphosphate kinase is a component of the Escherichia coli RNA degradosome

1997 • 123 citations

Analysis of mRNA decay and rRNA processing in Escherichia coli multiple mutants carrying a deletion in RNase III

1993 • 123 citations

17 Polynucleotide Phosphorylase

1982 • 121 citations

Surprises at the 3′ end of prokaryotic RNA

1995 • 112 citations

Specific endonucleolytic cleavage of the mRNA for ribosomal protein S20 of Escherichia coli requires the product of the ams gene in vivo and in vitro

1991 • 112 citations

Roles of RNase E, RNase II and PNPase in the degradation of the rpsO transcripts of Escherichia coli: stabilizing function of RNase II and evidence for efficient degradation in an ams pnp rnb mutant.

1994 • 111 citations

Genetic studies of cleavage‐initiated mRNA decay and processing of ribosomal 9S RNA show that the Escherichia coli ams and rne loci are the same

1991 • 101 citations

The Decay of Bacterial Messenger RNA

1996 • 93 citations

Poly(A)- and poly(U)-specific RNA 3′ tail shortening by E. coli ribonuclease E

1998 • 93 citations

The rpsO mRNA of Escherichia coli is polyadenylated at multiple sites resulting from endonucleolytic processing and exonucleolytic degradation.

1996 • 89 citations

RNase III cleavages in non-coding leaders of Escherichia coli transcriptscontrol mRNA stability and genetic expression

1990 • 83 citations

Secondary structure of the mRNA for ribosomal protein S20. Implications for cleavage by ribonuclease E.

1992 • 83 citations

Differential Sensitivities of Portions of the mRNA for Ribosomal Protein S20 to 3′-Exonucleases Dependent on Oligoadenylation and RNA Secondary Structure

1996 • 78 citations

RNase E activity is conferred by a single polypeptide: overexpression, purification, and properties of the ams/rne/hmp1 gene product.

1993 • 77 citations

Overexpression, Purification, and Properties of Escherichia coli Ribonuclease II

1996 • 70 citations

The Role of RNA Structure in Determining RNase E-dependent Cleavage Sites in the mRNA for Ribosomal Protein S20 in vitro

1993 • 61 citations

Poly(A) RNA in Escherichia coli: nucleotide sequence at the junction of the lpp transcript and the polyadenylate moiety.

1992 • 59 citations

Amplification of ribonuclease II(mb) activity inEscherichia coliK-12

1983 • 54 citations

Development of an in vitro mRNA decay system for Escherichia coli : Poly(A) polymerase I is necessary to trigger degradation

1996 • 51 citations

Decay of the IS 10 antisense RNA by 3′ exoribonucleases: evidence that RNase II stabilizes RNA‐OUT against PNPase attack

1994 • 48 citations

The pcnB gene of Escherichia coli, which is required for ColE1 copy number maintenance, is dispensable

1993 • 46 citations

Stabilization of the 3' one-third of Escherichia coli ribosomal protein S20 mRNA in mutants lacking polynucleotide phosphorylase

1989 • 45 citations

Multiple degradation pathways of the rpsO mRNA of Escherichia coli. RNase E interacts with the 5′ and 3′ extremeties of the primary transcript

1996 • 44 citations

Turnover of mRNA in prokaryotes and lower eukaryotes

1992 • 39 citations

Identification of a Second Poly(A) Polymerase in Escherichia coli

1994 • 37 citations

Modulation of the Activity of RNase E in Vitro by RNA Sequences and Secondary Structures 5′ to Cleavage Sites

1997 • 36 citations

Influence of translational efficiency on the stability of the mRNA for ribosomal protein S20 in Escherichia coli

1994 • 34 citations

Coupling between mRNA synthesis and mRNA stability in Escherichia coli

1994 • 23 citations