RNase E activity is conferred by a single polypeptide: overexpression, purification, and properties of the ams/rne/hmp1 gene product.

Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes

1980 • 1,256 citations

RNA recognition: towards identifying determinants of specificity

1991 • 739 citations

Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on western blots by monoclonal antibodies

1986 • 539 citations

Mechanisms of mRNA decay in bacteria: a perspective

1988 • 290 citations

A conditional lethal mutation in an Escherichia coli strain with a longer chemical lifetime of messenger RNA

1979 • 241 citations

Control of RNase E-mediated RNA degradation by 5′-terminal base pairing in E. coil

1992 • 212 citations

Specificity of Escherichia coli endoribonuclease RNase E: in vivo and in vitro analysis of mutants in a bacteriophage T4 mRNA processing site.

1992 • 211 citations

RNase E, an endoribonuclease, has a general role in the chemical decay of Escherichia coli mRNA: evidence that rne and ams are the same genetic locus

1990 • 209 citations

RNase E, an RNA processing enzyme from Escherichia coli.

1979 • 178 citations

Specific endonucleolytic cleavage of the mRNA for ribosomal protein S20 of Escherichia coli requires the product of the ams gene in vivo and in vitro

1991 • 112 citations

The gene specifying RNase E (rne) and a gene affecting mRNA stability (ams) are the same gene

1991 • 101 citations

Genetic studies of cleavage‐initiated mRNA decay and processing of ribosomal 9S RNA show that the Escherichia coli ams and rne loci are the same

1991 • 101 citations

Secondary structure of the mRNA for ribosomal protein S20. Implications for cleavage by ribonuclease E.

1992 • 83 citations

Structural requirements for the processing of Escherichia coli 5 S ribosomal RNA by RNase E in vitro

1992 • 82 citations

RNase PH: an Escherichia coli phosphate-dependent nuclease distinct from polynucleotide phosphorylase.

1988 • 77 citations

Analysis of the altered mRNA stability (ams) gene from Escherichia coli. Nucleotide sequence, transcriptional analysis, and homology of its product to MRP3, a mitochondrial ribosomal protein from Neurospora crassa.

1991 • 70 citations

Location of the RNA‐processing enzymes RNase III, RNase E and RNase P in the Eschenchia coli cell

1991 • 68 citations

Functional interaction of heat shock protein GroEL with an RNase E-like activity in Escherichia coli.

1993 • 62 citations

Maturatio of 5‐S rRNA: Ribonuclease E Cleavages and Their Dependence on Precursor Sequences

1983 • 58 citations

Turnover of mRNA in prokaryotes and lower eukaryotes

1992 • 39 citations

Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12

1989 • 38 citations

Sequencing and expression of thernegene of Escherichiacoli

1991 • 26 citations

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