High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

1992 • 4,683 citations

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

1978 • 4,472 citations

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

1977 • 3,829 citations

Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli

1983 • 1,241 citations

The tac promoter: a functional hybrid derived from the trp and lac promoters.

1983 • 1,001 citations

The purification of eukaryotic polypeptides synthesized in Escherichia coli

1986 • 838 citations

Identification and characterization of conserved and variable regions in the envelope gene of HTLV-III/LAV, the retrovirus of AIDS

1986 • 780 citations

Easy identification of cDNA clones.

1983 • 587 citations

Derivations and genotypes of some mutant derivatives of Escherichia coli K12

1987 • 582 citations

Production of abnormal proteins in E. coli stimulates transcription of ion and other heat shock genes

1985 • 521 citations

Synthesis of Guanosine Tetra- and Pentaphosphate Requires the Presence of a Codon-Specific, Uncharged Transfer Ribonucleic Acid in the Acceptor Site of Ribosomes

1973 • 466 citations

Regulation of ribosomal RNA promoters with a synthetic lac operator.

1984 • 451 citations

‘ATG vectors’ for regulated high-level expression of cloned genes in Escherichia coli

1985 • 392 citations

Codon usage can affect efficiency of translation of genes inEscherichia coli

1984 • 290 citations

DNA sequence for a low-level promoter of the lac repressor gene and an ‘up’ promoter mutation

1978 • 251 citations

Toxicity of an overproduced foreign gene product in Escherichia coli and its use in plasmid vectors for the selection of transcription terminators

1984 • 172 citations

Translation of the sequence AGG-AGG yields 50% ribosomal frameshift.

1988 • 157 citations

Control of formation of active soluble or inactive insoluble baker's yeast α-glucosidase PI in Escherichia coli by induction and growth conditions

1989 • 114 citations

The E. coli dnaY gene encodes an arginine transfer RNA

1986 • 98 citations

The AGG codon is translated slowly inE. colieven at very low expression levels

1988 • 67 citations

Production of thermostable, recombinant α-galactosidase suitable for raffinose elimination from sugar beet syrup

1988 • 61 citations

Variations in codon usage are not correlated with heterologous gene expression in Saccharomyces cerevisiae and Escherichia coli

1988 • 27 citations

Molecular Cloning, Sequencing, and Expression inEscherichia coliof Human Preprourokinase cDNA

1985 • 24 citations

Suppression of mutations conferring penicillin tolerance by interference with the stringent control mechanism of Escherichia coli

1987 • 21 citations

DNA‐Neukombination: eine praktische Anwendung in der Zuckerindustrie

1983 • 12 citations

Host requirements for growth of lambda-P22 hybrid in Escherichia coli

1981 • 2 citations