Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Variation of the CGG repeat at the fragile X site results in genetic instability: Resolution of the Sherman paradox

1991 • 2,062 citations

Absence of expression of the FMR-1 gene in fragile X syndrome

1991 • 1,456 citations

Physiological and genetic responses of bacteria to osmotic stress

1989 • 1,433 citations

PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.

1994 • 1,043 citations

The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.

1988 • 670 citations

Effective amplification of long targets from cloned inserts and human genomic DNA.

1994 • 646 citations

High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus

1991 • 569 citations

Increased thermal stability of proteins in the presence of naturally occurring osmolytes

1992 • 437 citations

Formamide can dramatically improve the specificity of PCR

1990 • 314 citations

High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity.

1993 • 286 citations

Betaine can eliminate the base pair composition dependence of DNA melting

1993 • 282 citations

Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase—an extremely heat stable enzyme with proofreading activity

1991 • 227 citations

Alteration of the Relative Stability of dA·dT and dG·dC Base Pairs in DNA

1973 • 212 citations

The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion

1992 • 207 citations

Structure-independent DNA amplification by PCR using 7-deaza-2'-deoxyguanosine

1988 • 148 citations

Organic solvents as facilitators of polymerase chain reaction.

1991 • 135 citations

Low concentrations of tetramethylammonium chloride increase yield and specificity of PCR

1995 • 128 citations

A specificity enhancer for polymerase chain reaction

1990 • 122 citations

Robust amplification and ethidium‐visible detection of the fragile X syndrome CGG repeat using Pfu polymerase

1994 • 81 citations

PCR-based detection of a polymorphic BamHI site in intron 1 of the human retinoblastoma (RB) gene

1990 • 70 citations

A rapid, non-radioactive screening test for fragile X mutations at the FRAXA and FRAXE loci.

1995 • 59 citations

Improved sizing of fragile X CCG repeats by nested polymerase chain reaction

1994 • 38 citations

Long-distance PCR.

1994 • 38 citations

Differential expression of four members of the POU family of proteins in activated and phorbol 12-myristate 13-acetate-treated Jurkat T cells.

1993 • 30 citations

A simple fragile X PCR assay with 7-deazaguanine-substituted DNA visualized by ethidium bromide

1994 • 9 citations

Purification of BsuE methyltransferase and its application in genome mapping

1991 • 4 citations