Analysis of Protein–Peptide Interaction by a Miniaturized Fluorescence Polarization Assay Using Cyclin-Dependent Kinase 2/Cyclin E as a Model System

A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays

1999 • 6,580 citations

Functional Inactivation of the Retinoblastoma Protein Requires Sequential Modification by at Least Two Distinct Cyclin-cdk Complexes

1998 • 1,058 citations

[17] The kinetics of reversible tight-binding inhibition

1979 • 679 citations

Polarisation de la lumière de fluorescence. Vie moyenne des molécules dans l'etat excité

1926 • 617 citations

Structural basis of cyclin-dependent kinase activation by phosphorylation

1996 • 585 citations

Cyclin-Binding Motifs Are Essential for the Function of p21^CIP1

1996 • 313 citations

Regulation of transcription by proteins that control the cell cycle

1997 • 220 citations

Cdk2 Kinase Is Required for Entry into Mitosis as a Positive Regulator of Cdc2–Cyclin B Kinase Activity

1996 • 214 citations

Fluorescence polarization — a new tool for cell and molecular biology

1995 • 185 citations

p27/Kip1 mutation found in breast cancer.

1996 • 174 citations

Deletional, mutational, and methylation analyses of CDKN2 (p16/MTS1) in primary and metastatic prostate cancer

1997 • 174 citations

Analysis of cyclin E and CDK2 in ovarian cancer: Gene amplification and RNA overexpression

1998 • 133 citations

Application of fluorescence polarization to the antigen-antibody reaction

1964 • 120 citations

High Throughput Screening: The Discovery of Bioactive Substances

1997 • 105 citations

Deletion of RBExons 24 and 25 Causes Low-Penetrance Retinoblastoma

1997 • 99 citations

A Fluorescence Polarization Based Src-SH2 Binding Assay

1997 • 88 citations

A Fluorescence Polarization Competition Immunoassay for Tyrosine Kinases

1998 • 86 citations

Cyclin E/Cdk2 activity is controlled by different mechanisms in the G0 and G1 phases of the cell cycle.

1996 • 49 citations

Fluorescence Polarization Assays for the Detection of Proteases and Their Inhibitors

1996 • 46 citations

A new protease activity assay using fluorescence polarization.

1994 • 46 citations

Fluorescence polarization a general method for measuring ligand binding and membrane microviscosity

1985 • 41 citations

Homogeneous detection of nucleic acids by transient-state polarized fluorescence.

1993 • 31 citations

Current and Future Trends in High Throughput Screening for Drug Discovery

1998 • 30 citations

Homogeneous detection of nucleic acids by transient-state polarized fluorescence

1993 • 30 citations

The amino terminus of Cdk2 binds p21.

1996 • 5 citations

Putative Nuclear cdk2 Substrates in Normal and Transformed Cells

1996 • 3 citations