Molecular analysis of chicken embryo SPARC (osteonectin)

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

A comprehensive set of sequence analysis programs for the VAX

1984 • 14,824 citations

Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins

1978 • 4,566 citations

Prediction of protein antigenic determinants from amino acid sequences.

1981 • 3,715 citations

Prediction of the Secondary Structure of Proteins from their Amino Acid Sequence

1979 • 2,930 citations

Osteonectin, a bone-specific protein linking mineral to collagen

1981 • 1,131 citations

Structure and biological activity of basement membrane proteins

1989 • 967 citations

Prosite: a dictionary of sites and patterns in proteins

1992 • 952 citations

Chemistry and Biology of Mineralized Tissues

2008 • 914 citations

An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

1990 • 677 citations

The antigenic index: a novel algorithm for predicting antigenic determinants

1988 • 655 citations

Extracellular proteins that modulate cell-matrix interactions. SPARC, tenascin, and thrombospondin

1991 • 560 citations

[9] Profile analysis

1990 • 486 citations

SPARC, a secreted protein associated with cellular proliferation, inhibits cell spreading in vitro and exhibits Ca+2-dependent binding to the extracellular matrix.

1989 • 375 citations

Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell ‘culture shock’ glycoprotein of Mr 43,000.

1986 • 315 citations

In vivo expression of mRNA for the Ca++-binding protein SPARC (osteonectin) revealed by in situ hybridization.

1987 • 292 citations

Characterization of a novel serum albumin-binding glycoprotein secreted by endothelial cells in culture.

1984 • 264 citations

Inhibition of angiogenesis and tumor growth in the brain. Suppression of endothelial cell turnover by penicillamine and the depletion of copper, an angiogenic cofactor.

1990 • 230 citations

The Ca2(+)-binding glycoprotein SPARC modulates cell cycle progression in bovine aortic endothelial cells.

1991 • 223 citations

Calcium binding domains and calcium-induced conformational transition of SPARC/BM-40/osteonectin, an extracellular glycoprotein expressed in mineralized and nonmineralized tissues

1987 • 211 citations

Chicken ovomucoid: determination of its amino acid sequence, determination of the trypsin reactive site, and preparation of all three of its domains

1987 • 202 citations

Ovomucoid third domains from 100 avian species: isolation, sequences, and hypervariability of enzyme-inhibitor contact residues

1987 • 202 citations

Distribution of the calcium-binding protein SPARC in tissues of embryonic and adult mice.

1989 • 192 citations

Osteonectin cDNA sequence reveals potential binding regions for calcium and hydroxyapatite and shows homologies with both a basement membrane protein (SPARC) and a serine proteinase inhibitor (ovomucoid).

1988 • 189 citations

Calcium Coordination and the Calmodulin Fold: Divergent versus Convergent Evolution

1987 • 184 citations

DNA Sequencing Markets

1990 • 173 citations

Stimulation of collagen synthesis in fibroblast cultures by the tripeptide‐copper complex glycyl‐L‐histidyl‐L‐lysine‐Cu²⁺

1988 • 170 citations

Functional mapping of SPARC: peptides from two distinct Ca+(+)-binding sites modulate cell shape.

1990 • 164 citations

Molecular analysis of the cDNA for human SPARC/osteonectin/BM-40: Sequence, expression, and localization of the gene to chromosome 5q31-q33

1988 • 157 citations

Purification and tissue distribution of a small protein (BM-40) extracted from a basement membrane tumor

1986 • 157 citations

Molecular cloning of SC1: A putative brain extracellular matrix glycoprotein showing partial similarity to osteonectin/BM40/SPARC

1990 • 153 citations

SPARC antagonizes the effect of basic fibroblast growth factor on the igration of bovine aortic endothelial cells

1992 • 142 citations

Differential effects of SPARC and cationic SPARC peptides on DNA synthesis by endothelial cells and fibroblasts

1993 • 139 citations

Modulation of extracellular matrix proteins by endothelial cells undergoing angiogenesis in vitro.

1991 • 134 citations

Solubilization of protein BM‐40 from a basement membrane tumor with cheating agents and evidence for its identity with osteonectin and SPARC

1987 • 128 citations

Gene expression in mineralizing chick epiphyseal cartilage.

1988 • 109 citations

Calcium‐dependent binding of basement membrane protein BM‐40 (osteonectin, SPARC) to basement membrane collagen type IV

1991 • 107 citations

Endothelial cell injury in vitro is associated with increased secretion of an M_r 43,000 glycoprotein ligand

1986 • 99 citations

Recombinant expression and properties of the human calcium‐binding extracellular matrix protein BM‐40

1991 • 99 citations

Developmental expression of genes in chick growth cartilage detected byin situ hybridization

1989 • 97 citations

Structure of human osteonectin based upon analysis of cDNA and genomic sequences

1989 • 83 citations

Characterization of the mouse SPARC/osteonectin gene. Intron/exon organization and an unusual promoter region.

1988 • 82 citations

High‐affinity and low‐affinity calcium binding and stability of the multidomain extracellular 40‐kDa basement membrane glycoprotein (BM‐40/SPARC/osteonectin)

1992 • 79 citations

The collagen binding specificity of bone and platelet osteonectin is related to differences in glycosylation

1991 • 78 citations

Distinct patterns of glycosylation of colligin, a collagen‐binding glycoprotein, and SPARC (osteonectin), a secreted Ca²⁺‐binding glycoprotein

1987 • 77 citations

Cloning and complete amino acid sequences of human and murine basement membrane protein BM‐40 (SPARC, osteonectin)

1988 • 76 citations

Hypertrophic Chondrocytes

1990 • 71 citations

Transcription of a quail gene expressed in embryonic retinal cells is shut off sharply at hatching.

1991 • 67 citations

[28] Biological activity of human plasma copper-binding growth factor glycyl-l-histidyl-l-lysine

1987 • 57 citations

Isolation and chemical characterization of the phosphoproteins of chicken bone matrix: heterogeneity in molecular weight and composition

1986 • 47 citations

The Calcium-Binding Protein SPARC is Secreted by Leydig and Sertoli Cells of the Adult Mouse Testis1

1989 • 44 citations

Isolation of the osteonectin gene: evidence that a variable region of the osteonectin molecule is encoded within one exon

1988 • 43 citations

Heat‐shock response in cultured chick embryo chondrocytes

1992 • 42 citations

Radioimmunoassay for osteonectin. Concentrations in bone, nonmineralized tissues, and blood

1987 • 41 citations

Molecular analysis of Xenopus laevis SPARC (Secreted Protein, Acidic, Rich in Cysteine). A highly conserved acidic calcium-binding extracellular-matrix protein

1992 • 37 citations