Expression, purification, and characterization of the Drosophila kinesin motor domain produced in Escherichia coli

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane

1971 • 8,619 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

Spectroscopic Determination of Tryptophan and Tyrosine in Proteins^*

1967 • 3,423 citations

Microtubule Assembly in the Absence of Added Nucleotides

1973 • 2,055 citations

Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility

1985 • 1,930 citations

A simplified method for the quantitative assay of small amounts of protein in biologic material

1973 • 1,802 citations

Vectors for selective expression of cloned DNAs by T7 RNA polymerase

1987 • 1,449 citations

A novel brain ATPase with properties expected for the fast axonal transport motor

1985 • 604 citations

Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration

1989 • 460 citations

Microtubule-associated proteins and the stimulation of tubulin assembly in vitro

1976 • 434 citations

A three-domain structure of kinesin heavy chain revealed by DNA sequence and microtubule binding analyses

1989 • 392 citations

Intracellular Transport Using Microtubule-Based Motors

1987 • 356 citations

Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle

1985 • 301 citations

Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide

1988 • 284 citations

Kinesin ATPase: rate-limiting ADP release.

1988 • 238 citations

Identification of globular mechanochemical heads of kinesin

1989 • 231 citations

Evidence that the Head of Kinesin Is Sufficient for Force Generation and Motility in Vitro

1990 • 225 citations

Bovine brain kinesin is a microtubule-activated ATPase.

1986 • 215 citations

Kinesin heavy chain is essential for viability and neuromuscular functions in Drosophila, but mutants show no defects in mitosis

1991 • 197 citations

Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells

1992 • 193 citations

The quaternary structure of bovine brain kinesin.

1988 • 178 citations

Characterization of the microtubule movement produced by sea urchin egg kinesin.

1987 • 153 citations

Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm [published erratum appears in J Cell Biol 1991 Aug;114(4):following 863]

1991 • 112 citations

Quantitative analysis of sea urchin egg kinesin-driven microtubule motility

1989 • 111 citations

The kinesin superfamily: tails of functional redundancy

1991 • 103 citations

Evidence that the stalk of Drosophila kinesin heavy chain is an alpha-helical coiled coil.

1992 • 99 citations

The primary structure and analysis of the squid kinesin heavy chain.

1990 • 92 citations

Isolation and characterization of the gene encoding the heavy chain of Drosophila kinesin.

1988 • 92 citations

Activation of the dynein adenosinetriphosphatase by microtubules. Kinetics of ATP-induced dissociation and re-formation of the microtubule-dynein complex

1986 • 92 citations

Decoration of the microtubule surface by one kinesin head per tubulin heterodimer

1993 • 79 citations

Characterization of α2β2 and α2 forms of kinesin

1991 • 77 citations

A kinetic study of the kinesin ATPase.

1992 • 72 citations

Nucleotide-free Kinesin Hydrolyzes ATP with Burst Kinetics

1989 • 40 citations