Analysis of Four Residues within Substrate Recognition Site 4 of Human Cytochrome P450 3A4: Role in Steroid Hydroxylase Activity and α-Naphthoflavone Stimulation

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

High-resolution crystal structure of cytochrome P450cam

1987 • 1,396 citations

Substrate recognition sites in cytochrome P450 family 2 (CYP2) proteins inferred from comparative analyses of amino acid and coding nucleotide sequences.

1992 • 1,168 citations

Structure and function of cytochromes P450:a comparative analysis of three crystal structures

1995 • 660 citations

Cytochrome P-450 hPCN3, a Novel Cytochrome P-450 IIIA Gene Product That Is Differentially Expressed in Adult Human Liver

1989 • 480 citations

Alteration of mouse cytochrome P450coh substrate specificity by mutation of a single amino-acid residue

1989 • 382 citations

Cooperativity in Oxidations Catalyzed by Cytochrome P450 3A4

1997 • 380 citations

Structure of cytochrome P450eryF involved in erythromycin biosynthesis

1995 • 370 citations

Activation of CYP3A4: Evidence for the Simultaneous Binding of Two Substrates in a Cytochrome P450 Active Site

1994 • 348 citations

Crystal structure of the cytochrome P-450CAM active site mutant Thr252Ala

1991 • 205 citations

The structural basis for substrate-induced changes in redox potential and spin equilibrium in cytochrome P-450CAM

1989 • 181 citations

Alanine-scanning Mutagenesis of a Putative Substrate Recognition Site in Human Cytochrome P450 3A4

1997 • 148 citations

Modulation of rabbit and human hepatic cytochrome P-450-catalyzed steroid hydroxylations by alpha-naphthoflavone.

1988 • 143 citations

Correlation of human cytochrome P4502C substrate specificities with primary structure: warfarin as a probe.

1993 • 139 citations

Molecular modeling of cytochrome P450 3A4.

1997 • 129 citations

Site-directed mutageneses of rat liver cytochrome P-450d: catalytic activities toward benzphetamine and 7-ethoxycoumarin

1989 • 101 citations

Regioselective progesterone hydroxylation catalyzed by eleven rat hepatic cytochrome P-450 isozymes

1987 • 98 citations

Evidence That Aspartic Acid 301 Is a Critical Substrate-Contact Residue in the Active Site of Cytochrome P450 2D6

1995 • 95 citations

Molecular basis for a functionally unique cytochrome P450IIB1 variant.

1991 • 93 citations

Identification of Three Key Residues in Substrate Recognition Site 5 of Human Cytochrome P450 3A4 by Cassette and Site-Directed Mutagenesis

1997 • 77 citations

Engineering of cytochrome P450 2B1 specificity. Conversion of an androgen 16 beta-hydroxylase to a 15 alpha-hydroxylase.

1993 • 75 citations

Positive effectors of the binding of an active site-directed amino steroid to rabbit cytochrome P-450 3c.

1988 • 61 citations

Characterization of a phenobarbital-inducible dog liver cytochrome P450 structurally related to rat and human enzymes of the P450IIIA (steroid-inducible) gene subfamily

1989 • 61 citations

Structural function of residue-209 in coumarin 7-hydroxylase (P450coh). Enzyme-kinetic studies and site-directed mutagenesis.

1991 • 61 citations

Escherichia coli Expression of Site-Directed Mutants of Cytochrome P450 2B1 from Six Substrate Recognition Sites: Substrate Specificity and Inhibitor Selectivity Studies

1995 • 60 citations

Structural determinants of cytochrome P450 2B1 specificity: evidence for five substrate recognition sites

1994 • 59 citations

Elucidation of Amino Acid Residues Critical for Unique Activities of Rabbit Cytochrome P450 2B5 Using Hybrid Enzymes and Reciprocal Site-Directed Mutagenesis with Rabbit Cytochrome P450 2B4

1996 • 56 citations

Replacement of Thr–303 of P450 2E11 with Serine Modifies the Regioselectivity of Its Fatty Acid Hydroxylase Activity

1993 • 53 citations

Role of residue 478 as a determinant of the substrate specificity of cytochrome P450 2B1

1992 • 52 citations

Engineering mouse P450coh to a novel corticosterone 15 alpha-hydroxylase and modeling steroid-binding orientation in the substrate pocket.

1993 • 51 citations

Escherichia coli Expression and Characterization of Cytochromes P450 2B11, 2B1, and 2B5

1994 • 47 citations

Site-directed mutagenesis of putative substrate recognition sites in cytochrome P450 2B11: importance of amino acid residues 114, 290, and 363 for substrate specificity.

1994 • 47 citations

An inhibitory monoclonal antibody binds in close proximity to a determinant for substrate binding in cytochrome P450IIC5.

1991 • 43 citations

Identification and Characterization of a Novel Protein (p137) Which Transcytoses Bidirectionally in Caco-2 Cells

1995 • 42 citations

Active-Site Topologies of Human CYP2D6 and Its Aspartate-301 → Glutamate, Asparagine, and Glycine Mutants

1996 • 34 citations

Site-directed mutagenesis of cytochrome P450s CYP2A1 and CYP2A2: influence of the distal helix on the kinetics of testosterone hydroxylation

1992 • 32 citations

Mutagenesis Study of Asp-290 in Cytochrome P450 2B11 Using a Fusion Protein with Rat NADPH-Cytochrome P450 Reductase

1996 • 31 citations

Transcription analysis of the Staphylococcus aureus gene encoding penicillin-binding protein 4

1997 • 25 citations