Rapid site-specific mutagenesis in plasmids

pEMBL: a new family of single stranded plasmids

1983 • 1,231 citations

[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

1983 • 1,093 citations

Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

1985 • 818 citations

Changing the identity of a transfer RNA

1986 • 233 citations

Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.

1982 • 211 citations

Improvement Of Oligonucleotide-Directed Site-Specific Mutagenesis Using Double-Stranded Plasmid DNA

1984 • 189 citations

[4] Exonuclease III: Use for DNA sequence analysis and in specific deletions of nucleotides

1983 • 139 citations

Oligonucleotide-directed double-strand break repair in plasmids of Escherichia coli: a method for site-specific mutagenesis.

1986 • 55 citations

Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C.

1981 • 52 citations

Construction of a Systematic Set of tRNA Mutants by Ligation of Synthetic Oligonucleotides into Defined Single-Stranded Gaps

1986 • 17 citations