Amino acid analysis utilizing phenylisothiocyanate derivatives

On the Determination of Cystine as Cysteic Acid

1963 • 3,022 citations

PHOTOMETRIC NINHYDRIN METHOD FOR USE IN THE CHROMATOGRAPHY OF AMINO ACIDS

1948 • 2,986 citations

Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole Range

1972 • 2,632 citations

The Preparation and Enzymatic Hydrolysis of Reduced and S-Carboxymethylated Proteins

1963 • 2,600 citations

Rapid analysis of amino acids using pre-column derivatization

1984 • 2,491 citations

Chromatography of Amino Acids on Sulfonated Polystyrene Resins. An Improved System

1958 • 2,190 citations

Amino acid analysis by reverse-phase high-performance liquid chromatography: Precolumn derivatization with phenylisothiocyanate

1984 • 1,616 citations

Method for Determination of the Amino Acid Sequence in Peptides.

1950 • 1,360 citations

Fluorescence reaction for amino acids

1971 • 1,331 citations

Hydrolysis of Proteins with p-Toluenesulfonic Acid

1971 • 1,052 citations

Amino Acid Analysis and Enzymatic Sequence Determination of Peptides by an Improved<u>o</u>-Phthaldialdehyde Precolumn Labeling Procedure

1981 • 607 citations

Determination of amino acids with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography

1983 • 553 citations

Purification and characterization of a unique isozyme of cytochrome P-450 from liver microsomes of ethanol-treated rabbits.

1982 • 537 citations

o-phthaldialdehyde precolumn derivatization and reversed-phase high-performance liquid chromatography of polypeptide hydrolysates and physiological fluids

1983 • 525 citations

High performance liquid chromatographic determination of amino acids in the picomole range

1979 • 504 citations

An evaluation of the Waters Pico‐Tag system for the amino‐acid analysis of food materials

1986 • 493 citations

Amino acid analysis with fluorescamine at the picomole level

1973 • 377 citations

Rapid assay for amino acids in serum or urine by pre-column derivatization and reversed-phase liquid chromatography.

1982 • 368 citations

Amino acid sequence of human tumor derived angiogenin

1985 • 357 citations

Purification of two distinct growth factors from bovine neural tissue by heparin affinity chromatography

1984 • 298 citations

Methods in Protein Sequence Analysis

1989 • 295 citations

Column chromatography of amino acids with fluorescence detection

1973 • 291 citations

Liquid chromatographic determination of amino acids after gas-phase hydrolysis and derivatization with (dimethylamino)azobenzenesulfonyl chloride

1986 • 278 citations

Sequence analysis of phosphoserine‐containing peptides

1986 • 260 citations

The structure of the fluorescent adduct formed in the reaction of o-phthalaldehyde and thiols with amines

1976 • 246 citations

The o-phthalaldehyde derivatives of amines for high-speed liquid chromatography/electrochemistry

1984 • 243 citations

Measurement of free amino acids in human biological fluids by high-performance liquid chromatography

1984 • 240 citations

Modern methods in protein chemistry

1983 • 221 citations

PITC derivatives in amino acid analysis

1986 • 215 citations

The separation of o-phthalaldehyde derivatives of amino acids by reversed-phase chromatography on octylsilica columns

1986 • 200 citations

Profiling of amino acids in body fluids and tissues by means of liquid chromatography

1986 • 190 citations

Methods of protein microcharacterization

1987 • 146 citations

Automated fluorometric amino acid analysis: The determination of proline and hydroxyproline

1979 • 132 citations

High-resolution reversed-phase liquid chromatography system for the analysis of complex solutions of primary and secondary amino acids

1986 • 127 citations

High-sensitivity amino acid analysis: Methodology for the determination of amino acid compositions with less than 100 picomoles of peptides

1982 • 127 citations

Methods of Protein Microcharacterization

1986 • 126 citations

Trimethyllysine and protein function. Effect of methylation and mutagenesis of lysine 115 of calmodulin on NAD kinase activation.

1986 • 120 citations

The hydrolysis of proteins

1970 • 106 citations

Amino acid analysis by HPLC: Optimized conditions for chromatography of phenylthiocarbamyl derivatives

1986 • 106 citations

Determination of 27 dansyl amino acid derivatives in biological fluids by reversed-phase high-performance liquid chromatography

1986 • 103 citations

Application of high-performance liquid chromatography to the determination of free amino acids in physiological fluids

1984 • 102 citations

The Separation of Dansyl Amino Acids by Reversed-Phase High Performance Liquid Chromatography

1978 • 99 citations

The protein phosphatases involved in cellular regulation

1986 • 99 citations

High-performance liquid chromatographic peptide mapping and amino acid analysis in the sub-nanomole range

1986 • 86 citations

Automated Analysis of O-Phthalal-Dehyde Derivatives of Amino Acids in Physiological Fluids by Reverse Phase High Performance Liquid Chromatography

1985 • 76 citations

Sensitive detection of amino acids in human serum and dried blood disc of 3 mm diameter for diagnosis of inborn errors of metabolism

1984 • 67 citations

Separation of phenylthiocarbamyl amino acids by high-performance liquid chromatography on Spherisorb octadecylsilane columns

1985 • 66 citations

Amino acid sequence of bovine brain derived class 1 heparin-binding growth factor

1986 • 60 citations

Reductive methylation of lysine residues in acidic fibroblast growth factor: effect on mitogenic activity and heparin affinity

1988 • 60 citations

Sample Preparation Techniques Prior to HPLC Analysis of Serum Nucleosides and Their Bases

1979 • 44 citations

Amino acid analysis of feedstuff hydrolysates by precolumn derivatization with phenylisothiocyanate and reversed-phase high-performance liquid chromatography.

1987 • 42 citations

Studies on the dark/light regulation of maize leaf pyruvate, orthophosphate dikinase by reversible phosphorylation

1985 • 40 citations

Complete high-performance liquid chromatographic separation of 4-N,N-dimethylaminoazobenzene-4′-thiohydantoin and 4-dimethylaminoazobenzene-4′-sulphonyl chloride amino acids utilizing the same reversed-phase column at room temperature

1985 • 38 citations

Methods in Protein Sequence Analysis · 1986

1987 • 37 citations

Reversed-phase ion-pair chromatography of amino acids

1983 • 33 citations

Use of Pico-Tag methodology in the chemical analysis of peptides with carboxyl-terminal amides

1986 • 32 citations

High-speed liquid chromatographic analysis of amino acids by post-column sodium hypochlorite-o∼phthalaldehyde reaction

1985 • 32 citations

Analysis by reverse-phase high-pressure liquid chromatography of phenylisothiocyanate-derivatized 1-aminocyclopropane-1-carboxylic acid in apple extracts

1986 • 31 citations

Derivatization of secondary amino acids with 7-nitro-4-benzofurazanyl ethers

1982 • 30 citations

Reversed-phase ion-pair chromatography of amino acids. Application to the determination of amino acids in plasma samples and dried blood on filter papers.

1983 • 29 citations

Sensitive analysis of asparagine and glutamine in physiological fluids and cells by precolumn derivatization with phenylisothiocyanate and reversed-phase high-performance liquid chromatography

1986 • 28 citations

A convenient protein substrate for the determination of protease specificity: Reduced and S-3-(trimethylated amino)propylated lysozyme

1985 • 27 citations

The amino acid sequence of the blue copper protein of Alcaligenes faecalis

1986 • 26 citations

High-performance liquid chromatography of amino acids, peptides and proteins

1983 • 25 citations

Amino acid composition of proteins eluted from polyacrylamide gels: Background considerations

1980 • 25 citations

Human class 1 heparin-binding growth factor: structure and homology to bovine acidic brain fibroblast growth factor

1986 • 25 citations

Amino acid analysis using standard high-performance liquid chromatography equipment

1982 • 25 citations

Pre-column o-phthalaldehyde derivatization of amino acids and their separation using reversed-phase high-performance liquid chromatography

1984 • 24 citations

Use of narrow-bore high-performance liquid chromatography for microanalysis of protein structure

1986 • 24 citations

Fluorescent labelling of amino acids with 9-anthryldiazomethane and its applications to high-performance liquid chromatography

1985 • 23 citations

High-performance liquid chromatography of amino acids in urine and cerebrospinal fluid

1984 • 22 citations

Application to the determination of amino acid in plasma samples and dried blood on filter papers

1988 • 21 citations

High-sensitivity amino acid analysis of stained peptides and proteins from a sodium dedecyl sulphate-polyacrylamide slab gel

1986 • 20 citations

Isolation of pyrophosphohistidine from pyrophosphorylated pyruvate,phosphate dikinase

1986 • 19 citations

Rapid and sensitive method for the determination of proline by reversed-phase high-performance liquid chromatography with automated pre-column fluorescence derivatization

1985 • 18 citations

A picomole protein and peptide chemistry: Some applications to the opioid peptides

1984 • 17 citations

High‐Performance Liquid Chromatography: Analytic and Preparative Applications in Protein‐Structure Determination

1983 • 15 citations

Identification of tyrosine O-sulfate in proteins by reverse-phase high-performance liquid chromatography: Use of base hydrolysis combined with precolumn derivatization using phenyl isothiocyanate

1986 • 11 citations

High-performance liquid chromatographic column switching method for the determination of hydroxyproline in meat and meat products

1986 • 11 citations

Micro-analysis of amino acids

1982 • 7 citations

Multi-sample quantification of amino acids and imino acids with a single analytical system

1971 • 7 citations

Determination of β-aspartylpeptidase activity in human faeces by high-performance liquid chromatography using pre-column derivatization with phenyl isothiocyanate

1986 • 7 citations

Post-column reaction of amino acids with the pentane-2,4-dione—form-aldehyde system for their automated analysis

1985 • 5 citations

High Performance Liquid Chromatographic Assay for Alpha-Difluoromethylornithine in Rodent Feed

1984 • 3 citations

Determination of low levels of 4-fluorophenylalanine incorporation into proteins

1986 • 2 citations