Structural and thermodynamic consequences of burying a charged residue within the hydrophobic core of T4 lysozyme

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

AREAS, VOLUMES, PACKING, AND PROTEIN STRUCTURE

1977 • 2,631 citations

Serine Proteases: Structure and Mechanism of Catalysis

1977 • 1,238 citations

Calculating the electrostatic potential of molecules in solution: Method and error assessment

1988 • 1,096 citations

An efficient general-purpose least-squares refinement program for macromolecular structures

1987 • 871 citations

Haemoglobin: The structural changes related to ligand binding and its allosteric mechanism

1979 • 862 citations

Focusing of electric fields in the active site of Cu‐Zn superoxide dismutase: Effects of ionic strength and amino‐acid modification

1986 • 759 citations

Electrostatic Effects in Proteins

1978 • 734 citations

pH-Induced denaturation of proteins: a single salt bridge contributes 3-5 kcal/mol to the free energy of folding of T4 lysozyme

1990 • 503 citations

[3] Expression and nitrogen-15 labeling of proteins for proton and nitrogen-15 nuclear magnetic resonance

1989 • 455 citations

Calculation of electrostatic potentials in an enzyme active site

1987 • 449 citations

[16] Nonlinear least-squares analysis

1985 • 449 citations

Structure of bacteriophage T4 lysozyme refined at 1.7 Å resolution

1987 • 349 citations

On the calculation of electrostatic interactions in proteins

1985 • 331 citations

Contributions of hydrogen bonds of Thr 157 to the thermodynamic stability of phage T4 lysozyme

1987 • 300 citations

Contributions of engineered surface salt bridges to the stability of T4 lysozyme determined by directed mutagenesis

1991 • 238 citations

Genetic and structural analysis of the protein stability problem

1987 • 227 citations

Purification of Bacteriophage T4 Lysozyme

1968 • 225 citations

Prediction of electrostatic effects of engineering of protein charges

1987 • 220 citations

Mechanism of tertiary structural change in hemoglobin.

1977 • 212 citations

On the environment of ionizable groups in globular proteins

1984 • 145 citations

Second-site revertants of an inactive T4 lysozyme mutant restore activity by restructuring the active site cleft

1991 • 104 citations

Ionization behavior of the catalytic carboxyls of lysozyme. Effects of ionic strength

1972 • 98 citations

[27] Structure and thermal stability of phage T4 lysozyme

1987 • 91 citations

A mutant T4 lysozyme (Val 131 → Ala) designed to increase thermostability by the reduction of strain within an α‐helix

1990 • 83 citations

Structural and thermodynamic analysis of the packing of two α-helices in bacteriophage T4 lysozyme

1991 • 78 citations

A proton-detected heteronuclear chemical-shift correlation experiment with improved resolution and sensitivity

1990 • 77 citations

Structure of a thermostable disulfide-bridge mutant of phage T4 lysozyme shows that an engineered cross-link in a flexible region does not increase the rigidity of the folded protein

1990 • 76 citations

Electrostatic fields in the active sites of lysozymes.

1989 • 56 citations

Neutron Protein Crystallography: Advances in Methods and Applications

1983 • 36 citations

Nature of the Charge Destribution in Proteins. II. Effect of Atomic Partial Charges on Ionic Charges

1985 • 8 citations