Structural and thermodynamic analysis of the packing of two α-helices in bacteriophage T4 lysozyme

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Some Factors in the Interpretation of Protein Denaturation

1959 • 4,817 citations

AREAS, VOLUMES, PACKING, AND PROTEIN STRUCTURE

1977 • 2,631 citations

Stability of Proteins Small Globular Proteins

1979 • 2,139 citations

Solvation energy in protein folding and binding

1986 • 1,853 citations

Tertiary templates for proteins

1987 • 1,485 citations

The nature of the accessible and buried surfaces in proteins

1976 • 1,151 citations

Contribution of Hydrophobic Interactions to the Stability of the Globular Conformation of Proteins

1962 • 986 citations

An efficient general-purpose least-squares refinement program for macromolecular structures

1987 • 871 citations

Temperature dependence of the hydrophobic interaction in protein folding.

1986 • 865 citations

Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding.

1987 • 738 citations

[3] Expression and nitrogen-15 labeling of proteins for proton and nitrogen-15 nuclear magnetic resonance

1989 • 455 citations

Analysis of the relationship between side-chain conformation and secondary structure in globular proteins

1987 • 422 citations

Substantial increase of protein stability by multiple disulphide bonds

1989 • 415 citations

Contribution of hydrophobic interactions to protein stability

1988 • 413 citations

Alternative packing arrangements in the hydrophobic core of λrepresser

1989 • 375 citations

Hydrophobic stabilization in T4 lysozyme determined directly by multiple substitutions of Ile 3

1988 • 375 citations

Contributions of the large hydrophobic amino acids to the stability of staphylococcal nuclease

1990 • 372 citations

Structure of bacteriophage T4 lysozyme refined at 1.7 Å resolution

1987 • 349 citations

Energetics of complementary side chain packing in a protein hydrophobic core

1989 • 346 citations

Enhanced protein thermostability from designed mutations that interact with α-helix dipoles

1988 • 290 citations

Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation

1986 • 275 citations

Temperature-sensitive mutations of bacteriophage T4 lysozyme occur at sites with low mobility and low solvent accessibility in the folded protein

1987 • 253 citations

Energy refinement of hen egg-white lysozyme

1974 • 233 citations

Purification of Bacteriophage T4 Lysozyme

1968 • 225 citations

Molecular basis of thermostability in the lysozyme from bacteriophage T4

1979 • 212 citations

Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding

1989 • 203 citations

GENETIC ANALYSIS OF STAPHYLOCOCCAL NUCLEASE: IDENTIFICATION OF THREE INTRAGENIC "GLOBAL" SUPPRESSORS OF NUCLEASE-MINUS MUTATIONS

1985 • 189 citations

Influence of Interior Packing and Hydrophobicity on the Stability of a Protein

1989 • 173 citations

Hydrophobic packing in T4 lysozyme probed by cavity-filling mutants.

1989 • 160 citations

Reverse hydrophobic effects relieved by amino-acid substitutions at a protein surface

1990 • 157 citations

Thermodynamic stability and point mutations of bacteriophage T4 lysozyme

1984 • 149 citations

The Influence of Amino Acid Side Chains on the Free Energy of Helix-Coil Transitions¹

1966 • 138 citations

Replacements of Pro ⁸⁶ in Phage T4 Lysozyme Extend an α-Helix But Do Not Alter Protein Stability

1988 • 131 citations

Cumulative effect of intragenic amino-acid replacements on the thermostability of a protein

1986 • 128 citations

Stability of phage T4 lysozymes I. Native properties and thermal stability of wild type and two mutant lysozymes

1977 • 125 citations

An empirical approach to protein conformation stability and flexibility

1983 • 116 citations

Stabilization of λ repressor against thermal denaturation by site‐directed Gly→Ala changes in α‐helix 3

1986 • 115 citations

[27] Structure and thermal stability of phage T4 lysozyme

1987 • 91 citations

An oscillation data collection system for high-resolution protein crystallography

1981 • 84 citations

A mutant T4 lysozyme (Val 131 → Ala) designed to increase thermostability by the reduction of strain within an α‐helix

1990 • 83 citations

A scanning calorimetric study of the thermal denaturation of the lysozyme of phage T4 and the Arg 96 .fwdarw. His mutant form thereof

1989 • 77 citations

Structural Studies of Mutants of T4 Lysozyme That Alter Hydrophobic Stabilization

1989 • 67 citations

Structural studies of mutants of the lysozyme of bacteriophage T4

1987 • 65 citations

The role of solvent polarity in the free energy of transfer of amino acid side chains from water to organic solvents.

1986 • 61 citations

Thermal denaturation of bacteriophage T4 lysozyme at neutral pH

1987 • 58 citations

Comparison of the crystal structure of bacteriophage T4 lysozyme at low, medium, and high ionic strengths

1991 • 55 citations

Mutations and protein stability

1981 • 54 citations

High-resolution structure of the temperature-sensitive mutant of phage lysozyme, Arg 96 .fwdarw. His

1989 • 39 citations

Structural analysis of the temperature-sensitive mutant of bacteriophage T4 lysozyme, glycine 156----aspartic acid.

1987 • 28 citations

Structural analysis of the temperature-sensitive mutant of bacteriophage T4 lysozyme, glycine 156→aspartic acid.

1987 • 26 citations

Crystallographic data for lysozyme from bacteriophage T4

1973 • 25 citations