Development of a database of amino acid sequences for human colon carcinoma proteins separated by two‐dimensional polyacrylamide gel electrophoresis

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

High resolution two-dimensional electrophoresis of proteins.

1975 • 19,515 citations

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

1987 • 5,066 citations

[17] Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis

1983 • 1,115 citations

Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties.

1985 • 1,081 citations

Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea

1986 • 926 citations

Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.

1987 • 819 citations

Two dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines.

1979 • 759 citations

Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns.

1985 • 706 citations

Identity of the proliferating cell nuclear antigen and cyclin

1984 • 702 citations

High resolution two-dimensional electrophoresis of human plasma proteins.

1977 • 659 citations

Electroblotting onto activated glass. High efficiency preparation of proteins from analytical sodium dodecyl sulfate-polyacrylamide gels for direct sequence analysis.

1986 • 440 citations

Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene.

1987 • 420 citations

Reproducible high yield sequencing of proteins electrophoretically separated and transferred to an inert support.

1988 • 375 citations

Automated amino acid sequence of small peptides utilizing Polybrene

1978 • 323 citations

A new siliconized‐glass fiber as support for protein‐chemical analysis of electroblotted proteins.

1988 • 319 citations

The QUEST System for Quantitative Analysis of Two-dimensional Gels

1989 • 308 citations

Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining

1988 • 305 citations

Methods in Protein Sequence Analysis

1989 • 295 citations

Protein-blotting on Polybrene-coated glass-fiber sheets. A basis for acid hydrolysis and gas-phase sequencing of picomole quantities of protein previously separated on sodium dodecyl sulfate/polyacrylamide gel

1985 • 288 citations

Human Gene Encoding the 78,000-Dalton Glucose-Regulated Protein and Its Pseudogene: Structure, Conservation, and Regulation

1988 • 236 citations

Structures of N-terminally acetylated proteins

1985 • 190 citations

Nucleotide sequence of a major messenger RNA for a 21 kilodalton polypeptide that is under translational control in mouse tumor cells

1988 • 183 citations

[16] Peptide mapping in one dimension by limited proteolysis of sodium dodecyl sulfate-solubilized proteins

1983 • 182 citations

Protein-electroblotting and -microsequencing strategies in generating protein data bases from two-dimensional gels.

1989 • 180 citations

Alterations in the phenotype of plant cells studied by NH ₂ -terminal amino acid-sequence analysis of proteins electroblotted from two-dimensional gel-separated total extracts

1987 • 178 citations

The Human Protein Index.

1982 • 165 citations

The Human Protein Index.

1982 • 161 citations

Gene-protein index of Escherichia coli K-12

1983 • 151 citations

Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase.

1986 • 143 citations

Amino acid sequence of mammalian elongation factor 2 deduced from the cDNA sequence: homology with GTP-binding proteins.

1986 • 141 citations

Degradation of organic nitrogen compounds by yeasts

1986 • 135 citations

Three expressed sequences within the human β-tubulin multigene family each define a distinct isotype

1985 • 134 citations

Identification of mouse brain proteins after two‐dimensional electrophoresis and electroblotting by microsequence analysis and amino acid composition analysis

1988 • 132 citations

A new colon carcinoma cell line (LIM1863) that grows as organoids with spontaneous differentiation into crypt-like structures in vitro.

1987 • 121 citations

Micropreparative procedures for high sensitivity sequencing of peptides and proteins

1989 • 110 citations

Sequencing of proteins from two-dimensional gels by using in situ digestion and transfer of peptides to polyvinylidene difluoride membranes: application to proteins associated with sensitization in Aplysia.

1988 • 108 citations

The primary structures of non‐histone chromosomal proteins HMG 1 and 2

1980 • 105 citations

Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane

1988 • 101 citations

Transformation-sensitive and Growth-related Changes of Protein Synthesis in REF52 Cells

1989 • 96 citations

Electroblotting of individual polypeptides from SDS/polyacrylamide gels for direct sequence analysis

1987 • 86 citations

Molecular characterization of human Ro/SS-A antigen. Amino terminal sequence of the protein moiety of human Ro/SS-A antigen and immunological activity of a corresponding synthetic peptide.

1988 • 64 citations

Structural features and restricted expression of a human α-tubulin gene

1985 • 63 citations

Primary structure of human triosephosphate isomerase.

1984 • 63 citations

A high‐performance liquid chromatography procedure for recovering subnanomole amounts of protein from SDS‐gel electroeluates for gas‐phase sequence analysis

1987 • 59 citations

Computerized, comprehensive databases of cellular and secreted proteins from normal human embryonic lung MRC‐5 fibroblasts: Identification of transformation and/or proliferation sensitive proteins

1989 • 54 citations

Human Spleen Histone H2B

1979 • 50 citations

The human chromosomal protein HMG I contains two identical palindrome amino acid sequences

1987 • 45 citations

Covalent immobilization of proteins for high-sensitivity sequence analysis: electroblotting onto chemically activated glass from sodium dodecyl sulfate-polyacrylamide gels

1988 • 43 citations

Two-dimensional electrophoresis

1990 • 43 citations

Systematic analysis of the total proteins of a mammalian organism: Principles, problems and implications for sequencing the human genome

1989 • 42 citations

Internal amino acid sequecing of proteins by in situ cyanogen bromide cleavage in polyacrylamide gels

1990 • 39 citations

Genomically linked cellular protein databases derived from two‐dimensional polyacrylamide gel electrophoresis

1989 • 38 citations

Peptide mapping and internal sequencing of proteins electroblotted from two-dimensional gels onto polyvinylidene difluoride membranes

1989 • 37 citations

Towards establishing comprehensive databases of cellular proteins from transformed human epithelial amnion cells (AMA) and normal peripheral blood mononuclear cells.

1988 • 31 citations

Highlights of protein structural analysis

1989 • 25 citations

Partial amino acid sequence of porcine 1,25-dihydroxyvitamin D3 receptor isolated by immunoaffinity chromatography.

1988 • 21 citations

Human Spleen Histone H3. Isolation and Amino Acid Sequence1

1981 • 18 citations

PEPTIDE MAPPING AND INTERNAL SEQUENCING OF PROTEINS FROM ACRYLAMIDE GELS

1990 • 17 citations

Chromatographic fractionation of proteins at high organic solvent modifier concentrations

1989 • 15 citations

Development of Membrane Supports for the Solid-phase Sequence Analysis of Proteins and Peptides

1989 • 15 citations

Report of workshop on cellular protein databases derived from two‐dimensional polyacrylamide gel electrophoresis

1989 • 15 citations

Comparison of ribosomal protein S1 and the A‐protein from Escherichia coli

1979 • 13 citations

Development of a database of amino acid sequences for proteins identified and isolated on two‐dimensional polyacrylamide gels

1989 • 11 citations

Direct N‐terminal sequence analysis of rat liver plasma membrane glycoproteins separated by two‐dimensional polyacrylamide gel electrophoresis

1989 • 8 citations

An Improved Injection System for On-line High Sensitivity Phenylthiohydantoin Amino Acid Analysis

1989 • 7 citations

Application of Two-Dimensional Gel Electrophoresis in Studies of Gene Expression during Early Plant Development

1984 • 6 citations

Polymerase Chain Reaction Technology

2002 • 6 citations