Cytoplasmic viscosity near the cell plasma membrane: translational diffusion of a small fluorescent solute measured by total internal reflection-fluorescence photobleaching recovery

Principles of Optics

1959 • 19,287 citations

Stochastic Problems in Physics and Astronomy

1943 • 8,251 citations

Mobility measurement by analysis of fluorescence photobleaching recovery kinetics

1976 • 2,658 citations

How crowded is the cytoplasm?

1982 • 807 citations

Cell-substrate contacts illuminated by total internal reflection fluorescence.

1981 • 804 citations

Total Internal Reflection Fluorescence

1984 • 728 citations

Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy

1995 • 623 citations

Probing the structure of cytoplasm.

1986 • 328 citations

Properties and metabolism of the aqueous cytoplasm and its boundaries

1984 • 320 citations

Determinants of the translational mobility of a small solute in cell cytoplasm.

1993 • 295 citations

Low viscosity in the aqueous domain of cell cytoplasm measured by picosecond polarization microfluorimetry.

1991 • 250 citations

A novel fluorescence ratiometric method confirms the low solvent viscosity of the cytoplasm

1993 • 212 citations

Physical properties of cytoplasm

1994 • 210 citations

The cytomatrix: a short history of its study.

1984 • 169 citations

Mapping of fluorescence anisotropy in living cells by ratio imaging. Application to cytoplasmic viscosity

1990 • 149 citations

Structural organization of interphase 3T3 fibroblasts studied by total internal reflection fluorescence microscopy.

1985 • 138 citations

Fluorescence recovery after photobleaching (FRAP) experiments under conditions of uniform disk illumination. Critical comparison of analytical solutions, and a new mathematical method for calculation of diffusion coefficient D

1988 • 101 citations

Diffusion in the aqueous compartment.

1984 • 101 citations

Spatial and temporal organization of calcium signalling in hepatocytes

1991 • 101 citations

Cytoplasmic viscosity near the cell plasma membrane: measurement by evanescent field frequency-domain microfluorimetry

1993 • 98 citations

Topography of cell-glass apposition revealed by total internal reflection fluorescence of volume markers.

1985 • 94 citations

Order in supported phospholipid monolayers detected by the dichroism of fluorescence excited with polarized evanescent illumination

1984 • 92 citations

Second messengers regulate endosomal acidification in Swiss 3T3 fibroblasts.

1992 • 89 citations

Water near intracellular surfaces.

1984 • 89 citations

Polarized fluorescence photobleaching recovery for measuring rotational diffusion in solutions and membranes

1988 • 88 citations

Self-diffusion and probe diffusion in dilute and semidilute aqueous solutions of dextran

1991 • 86 citations

Tracer diffusion in F-actin and Ficoll mixtures. Toward a model for cytoplasm

1990 • 83 citations

General electromagnetic theory of total internal reflection fluorescence: the quantitative basis for mapping cell-substratum topography

1987 • 83 citations

Total internal reflection fluorescence (TIRF) microscopy I. Modelling cell contact region fluorescence

1990 • 81 citations

Reversible Photobleaching of Fluorescein Conjugates in Air‐Saturated Viscous Solutions: Singlet and Triplet State Quenching by Tryptophan

1996 • 52 citations

Protein rotational motion in solution measured by polarized fluorescence depletion

1986 • 50 citations

Fluorescent triplet probes for measuring the rotational diffusion of membrane proteins

1982 • 49 citations

Rotational diffusion of TEMPONE in the cytoplasm of Chinese hamster lung cells

1983 • 43 citations

Construction and evaluation of a frequency-domain epifluorescence microscope for lifetime and anisotropy decay measurements in subcellular domains

1991 • 34 citations

A polarized photobleaching study of DNA reorientation in agarose gels

1990 • 33 citations

Cell membrane fluidity in the intact kidney proximal tubule measured by orientation-independent fluorescence anisotropy imaging

1990 • 31 citations

Organic osmolytes increase cytoplasmic viscosity in kidney cells

1992 • 30 citations

Construction and performance of a photobleaching recovery apparatus with microsecond time resolution

1996 • 30 citations

SPONTANEOUS RECOVERY OF FLUORESCENCE BY PHOTOBLEACHED SURFACE‐ADSORBED PROTEINS

1995 • 28 citations

A polarized photobleaching study of chromatin reorientation in intact nuclei

1990 • 28 citations

Dissociation Kinetics between a Mouse Fc Receptor (Fc.gamma.RII) and IgG: Measurement by Total Internal Reflection with Fluorescence Photobleaching Recovery

1995 • 27 citations

Reversible binding kinetics of a cytoskeletal protein at the erythrocyte submembrane

1994 • 26 citations

Theory for measuring bivalent surface binding kinetics using total internal reflection with fluorescence photobleaching recovery

1994 • 26 citations

Deoxygenation affects fluorescence photobleaching recovery measurements of red cell membrane protein lateral mobility

1994 • 24 citations

Membrane and microfilament organization and vasopressin action in transporting epithelia.

1987 • 7 citations