Phospholipase C and haemolytic activities of Clostridium perfringens alpha-toxin cloned in Escherichia coli: sequence and homology with a Baciilus cereus phospholipase C

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

[2] New M13 vectors for cloning

1983 • 5,402 citations

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

1978 • 4,472 citations

A rapid boiling method for the preparation of bacterial plasmids

1981 • 3,334 citations

Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA

1972 • 2,842 citations

A system for shotgun DNA sequencing

1981 • 2,710 citations

Compilation and analysis ofEscherichia colipromoter DNA sequences

1983 • 2,228 citations

A reliable method for the recovery of DNA fragments from agarose and acrylamide gels

1981 • 916 citations

Translational Initiation in Prokaryotes

1981 • 785 citations

The Release of Enzymes by Osmotic Shock from Escherichia coli in Exponential Phase

1966 • 672 citations

A comprehensive sequence analysis program for the IBM personal computer

1984 • 605 citations

The biochemistry of bacterial toxins

1941 • 522 citations

Bacterial toxins and cell membranes

1978 • 471 citations

PROTEIN SECRETION IN ESCHERICHIA COLI

1985 • 334 citations

The transposon Tn1 as a probe for studying ColE1 structure and function

1977 • 297 citations

Phospholipase C assay using p-nitrophenylphosphorylcholine together with sorbitol and its application to studying the metal and detergent requirement of the enzyme

1976 • 282 citations

Evidence for use of rare codons in the dnaG gene and other regulatory genes of Escherichia coli.

1983 • 249 citations

Pharmacology of bacterial toxins

1986 • 232 citations

Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum

1985 • 183 citations

Antigenic variation among group A streptococcal M proteins. Nucleotide sequence of the serotype 5 M protein gene and its relationship with genes encoding types 6 and 24 M proteins.

1988 • 143 citations

Nucleotide sequence of the streptolysin O (SLO) gene: structural homologies between SLO and other membrane-damaging, thiol-activated toxins

1987 • 141 citations

Virulence Factors of Clostridium perfringens

1979 • 118 citations

Sequence of the cellulase gene ofClostridium thermoceltumcoding for endoglucanase B

1986 • 101 citations

Cloning and sequencing of the gene encoding the phosphatidylcholine-preferring phospholipase C of Bacillus cereus

1988 • 100 citations

Methods in microbiology Vol. 14

1984 • 94 citations

Cloning and expression in Escherichia coli of Clostridium thermocellum genes coding for amino acid synthesis and cellulose hydrolysis

1983 • 92 citations

Nucleotide sequence and expression of a phosphate-regulated gene encoding a secreted hemolysin of Pseudomonas aeruginosa

1986 • 84 citations

Phospholipase C from Clostridium perfringens: Preparation and characterization of homogeneous enzyme

1984 • 83 citations

Purification of Clostridium perfringens phospholipase C (α-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein

1974 • 82 citations

Characterization of a bacteriocinogenic plasmid from Clostridium perfringens and molecular genetic analysis of the bacteriocin-encoding gene

1986 • 79 citations

Cloning, nucleotide sequencing, and expression of tetanus toxin fragment C in Escherichia coli

1986 • 74 citations

Genetic analysis of K88-mediated adhesion of enterotoxigenic Escherichia coli

1981 • 73 citations

Construction and use of signal sequence selection vectors in Escherichia coli and Bacillus subtilis

1987 • 71 citations

Identification of gene products programmed by restriction endonuclease DNA fragments using an E. coli in vitro system

1981 • 63 citations

Structural features of multiple nifH-like sequences and very biased codon usage in nitrogenase genes of Clostridium pasteurianum

1986 • 56 citations

UV-Assay

1974 • 53 citations

Cloning and genetic analysis of serotype 5 M protein determinant of group A streptococci: evidence for multiple copies of the M5 determinant in the Streptococcus pyogenes genome

1985 • 51 citations

Interaction of Clostridium perfringens θ-haemolysin, a contaminant of commercial phospholipase C, with erythrocyte ghost membranes and lipid dispersions. A morphological study

1975 • 50 citations

Biological activities contaminating preparations of phospholipase C (α-toxin) from Clostridium perfringens

1973 • 50 citations

Protein Secretion in Bacilli

1986 • 45 citations

Effect ofClostridium perfringens phospholipase C (alpha-toxin) on the human diploid fibroblast membrane

1974 • 37 citations

Cloning and expression of the hydrogenase gene from Clostridium butyricum in Escherichia coli

1983 • 35 citations