Systemic administration of transforming growth factor-beta 2 prevents the impaired bone formation and osteopenia induced by unloading in rats.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Bone histomorphometry: Standardization of nomenclature, symbols, and units: Report of the asbmr histomorphometry nomenclature committee

1987 • 4,980 citations

Bone Histomorphometry : Standardization of Nomenclature, Symbols, and Units

1987 • 3,281 citations

The Transforming Growth Factor-beta Family

1990 • 3,028 citations

Transforming growth factor type beta: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro.

1986 • 2,878 citations

Transforming growth factor beta modulates the expression of collagenase and metalloproteinase inhibitor.

1987 • 1,243 citations

Growth factors in bone matrix. Isolation of multiple types by affinity chromatography on heparin-Sepharose.

1986 • 870 citations

Regulation of fibronectin and type I collagen mRNA levels by transforming growth factor-beta.

1987 • 736 citations

Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur.

1990 • 734 citations

A nuclear factor 1 binding site mediates the transcriptional activation of a type I collagen promoter by transforming growth factor-β

1988 • 620 citations

Transforming growth factor beta is a bifunctional regulator of replication and collagen synthesis in osteoblast-enriched cell cultures from fetal rat bone.

1987 • 619 citations

In VivoStimulation of Bone Formation by Transforming Growth Factor-β

1989 • 592 citations

Osteoblasts synthesize and respond to transforming growth factor-type beta (TGF-beta) in vitro.

1987 • 561 citations

Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: a proposed model for indirect mitogenesis involving autocrine activity.

1986 • 541 citations

Transforming growth factor-beta increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblasts.

1987 • 467 citations

Quantitative histological data on disuse osteoporosis

1974 • 435 citations

Effects of transforming growth factor β on bone nodule formation and expression of bone morphogenetic protein 2, osteocalcin, osteopontin, alkaline phosphatase, and type I collagen mRNA in long-term cultures of fetal rat calvarial osteoblasts

1994 • 328 citations

Transforming growth factor beta inhibits formation of osteoclast-like cells in long-term human marrow cultures.

1988 • 297 citations

Transforming growth factor beta inhibits bone resorption in fetal rat long bone cultures.

1988 • 262 citations

Transforming growth factor-beta in the regulation of fracture repair.

1990 • 239 citations

In vivo effects of human recombinant transforming growth factor β on bone turnover in normal mice

1990 • 235 citations

One systemic administration of transforming growth factor-beta 1 reverses age- or glucocorticoid-impaired wound healing.

1993 • 232 citations

Transforming growth factor-beta. Multiple actions and potential clinical applications

1989 • 229 citations

Gene expression in osteoblastic cells.

1991 • 228 citations

The Temporal Response of Bone to Unloading*

1986 • 224 citations

Increased trabecular bone mass in rats treated with human synthetic parathyroid hormone

1984 • 219 citations

Stimulation of Bone Matrix Appositionin Vitroby Local Growth Factors: A Comparison between Insulin-like Growth Factor I, Platelet-Derived Growth Factor, and Transforming Growth Factor β*

1990 • 216 citations

Transforming growth factor-beta and remodeling of bone.

1991 • 216 citations

Cellular responses to mechanical loading in vitro

1990 • 202 citations

Human Platelet-Derived Transforming Growth Factor-βStimulates Parameters of Bone Growth in Fetal Rat Calvariae*

1986 • 198 citations

Prostaglandin E₂Stimulates Insulin-Like Growth Factor I Synthesis in Osteoblast-Enriched Cultures from Fetal Rat Bone*

1991 • 197 citations

Type β transforming growth factor (TGFβ) regulation of alkaline phosphatase expression and other phenotype‐related mRNAs in osteoblastic rat osteosarcoma cells

1987 • 196 citations

Transforming Growth Factor-β Stimulates Bone Matrix Apposition and Bone Cell Replication in Cultured Fetal Rat Calvariae*

1990 • 192 citations

Loading-related increases in prostaglandin production in cores of adult canine cancellous bone in vitro: A role for prostacyclin in adaptive bone remodeling?

1991 • 191 citations

Tensile forces enhance prostaglandin E synthesis in osteoblastic cells grown on collagen ribbons

1984 • 184 citations

Hepatic processing of transforming growth factor beta in the rat. Uptake, metabolism, and biliary excretion.

1987 • 184 citations

Complete nucleotide sequence of mouse 18 S rRNA gene: comparison with other available homologs

1984 • 180 citations

TGF-β1 induces bone closure of skull defects: Temporal dynamics of bone formation in defects exposed to rhTGF-β1

1993 • 177 citations

Chemotactic response of osteoblastlike cells to transforming growth factorβ

1990 • 176 citations

Characterization and autoregulation of latent transforming growth factor beta (TGF beta) complexes in osteoblast-like cell lines. Production of a latent complex lacking the latent TGF beta-binding protein.

1994 • 173 citations

TGFβ alters growth and differentiation related gene expression in proliferating osteoblasts in vitro, preventing development of the mature bone phenotype

1994 • 139 citations

Insulin-like growth factor-I increases trabecular bone formation and osteoblastic cell proliferation in unloaded rats.

1994 • 138 citations

Stimulation of bone formation in vivo by transforming growth factor-β: Remodeling of woven bone and lack of inhibition by indomethacin

1990 • 121 citations

Transcriptional Regulation of Osteocalcin Production by Transforming Growth Factor-β in Rat Osteoblast-Like Cells

1989 • 114 citations

Characterization of cells with high alkaline phosphatase activity derived from human bone and marrow: Preliminary assessment of their osteogenicity

1985 • 110 citations

cDNA cloning of alkaline phosphatase from rat osteosarcoma (ROS 17/2.8) cells

1987 • 107 citations

Effects of transforming growth factor β and epidermal growth factor on cell proliferation and the formation of bone nodules in isolated fetal rat calvaria cells

1989 • 107 citations

Tissue Specificity of Type I Collagen Gene Expression Is Determined at Both Transcriptional and Post-Transcriptional Levels

1984 • 103 citations

Chemotactic response of osteoblast-like cells to transforming growth factor beta

1989 • 97 citations

Bone marrow from mechanically unloaded rat bones expresses reduced osteogenic capacity in vitro

1994 • 85 citations

Exogenous prostacyclin, but not prostaglandin E2, produces similar responses in both G6PD activity and RNA production as mechanical loading, and increases IGF-II release, in adult cancellous bone in culture

1993 • 83 citations

Differential chemotactic responses of different populations of fetal rat calvaria cells to platelet-derived growth factor and transforming growth factor β

1992 • 82 citations

DNA sequence comparison of the regulatory signals at the 5' end of the mouse and chick alpha 2 type I collagen genes.

1984 • 79 citations

Skeletal unloading in rat decreases proliferation of rat bone and marrow-derived osteoblastic cells

1993 • 78 citations

In vivo induction of bone by recombinant human transforming growth factor β1

1991 • 75 citations

Effects of transforming growth factor β1 on the regulation of osteoclastic development and function

1991 • 73 citations

Systemic administration of recombinant transforming growth factor beta 2 (rTGF-β2) stimulates parameters of cancellous bone formation in juvenile and adult rats

1994 • 73 citations

Correlation between mRNA levels for bone cell proteins and bone formation in long bones of maturing rats

1991 • 63 citations

Multiple regulatory effects by transforming growth factor-beta on type I collagen levels in osteoblast-enriched cultures from fetal rat bone.

1992 • 57 citations

Morphological study of recombinant human transforming growth factor β1-induced intramembranous ossification in neonatal rat parietal bone

1993 • 55 citations

Bone cell responsiveness to transforming growth factor β, parathyroid hormone, and prostaglandin E2 in normal and postmenopausal osteoporotic women

1990 • 51 citations

c-fos protooncogene is involved in the mitogenic effect of transforming growth factor-beta in osteoblastic cells.

1995 • 42 citations

Insulin-like growth factor-I increases trabecular bone formation and osteoblastic cell proliferation in unloaded rats

1994 • 35 citations

Increased proliferation of osteoblast precursor cells in estrogen-deficient rats

1993 • 33 citations

Effects of transforming growth factor type β on expression of cytoskeletal proteins in endosteal mouse osteoblastic cells☆

1990 • 32 citations

Disuse osteopenia is accompanied by downregulation of gene expression for bone proteins in growing rats

2006 • 30 citations

Dynamics of Circulating Osteocalcin in Rats During Growth and Under Experimental Conditions

1992 • 26 citations

In vivo effects of transforming growth factor-β2 in ovariectomized rats

1993 • 22 citations

Disuse osteopenia is accompanied by downregulation of gene expression for bone proteins in growing rats

1992 • 17 citations