Invertase gene (SUC2) of Saccharomyces cerevisiae as a dominant marker for transformation of Pichia pastoris

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels

1977 • 1,607 citations

Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase

1982 • 1,539 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

Yeast transformation: a model system for the study of recombination.

1981 • 1,491 citations

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells

1979 • 1,454 citations

The biochemistry of methylotrophs

1983 • 808 citations

Pichia pastoris as a Host System for Transformations

1985 • 495 citations

[76] β-d-Fructofuranoside fructohydrolase from yeast

1975 • 455 citations

Pichia pastoris as a host system for transformations.

1985 • 423 citations

Microbial growth on C1 compounds

1985 • 405 citations

Eukaryotic DNA segments capable of autonomous replication in yeast.

1980 • 366 citations

The biochemistry of methylotrophs

1983 • 365 citations

Expression of thelacZgene from two methanol-regulated promoters inPichia pastoris

1987 • 364 citations

High–Level Expression and Efficient Assembly of Hepatitis B Surface Antigen in the Methylotrophic Yeast, Pichia Pastoris

1987 • 332 citations

Growth of Hansenula polymorpha in a methanol-limited chemostat

1976 • 307 citations

Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris.

1985 • 271 citations

High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene

1979 • 269 citations

Autonomously replicating sequences in Saccharomyces cerevisiae.

1980 • 222 citations

Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the alcohol oxidase.

1980 • 190 citations

Microbial Assimilation of Methanol

1973 • 138 citations

Direct selection of Saccharomyces cerevisiae resistant to the antibiotic G418 following transformation with a DNA vector carrying the kanamycin-resistance gene of Tn903

1983 • 130 citations

Biosynthesis and regulation of the peroxisomal methanol oxidase from the methylotrophic yeast Hansenula polymorpha

1984 • 100 citations

Microbial Utilization of Methanol

1972 • 81 citations

Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn905

1984 • 76 citations

Biosynthesis of peroxisomal enzymes in the methylotrophic yeast Hansenula polymorpha

1983 • 72 citations

Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation

1983 • 70 citations

Saccharomyces cerevisiae spheroplasts are sensitive to the action of diphtheria toxin.

1982 • 14 citations