DISEC-TRISEC: di- and trinucleotide-sticky-end cloning of PCR-amplified DNA

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products

1991 • 1,182 citations

Ligation-independent cloning of PCR products (LIC-PCR)

1990 • 1,161 citations

Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases

1988 • 847 citations

Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences

1986 • 766 citations

General method for direct cloning of DNA fragments generated by the polymerase chain reaction

1991 • 137 citations

Restriction endonuclease cleavage at the termini of PCR products.

1990 • 90 citations

Efficient cloning of PCR generated DNA containing terminal restriction endonuclease recognition sites

1990 • 75 citations

Organization and structure of Volvox β-tubulin genes

1988 • 67 citations

PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products

1990 • 62 citations

DMA mutagenesis and recombination

1990 • 44 citations