Cloning, mapping and molecular analysis of the pyrG (orotidine-5'-phosphate decarboxylase) gene of Aspergillus nidulans

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome

1976 • 1,313 citations

Are snRNPs involved in splicing?

1980 • 1,206 citations

Isolation of the Candida albicans gene for orotidine-5′-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations

1984 • 1,198 citations

Transformation of Aspergillus nidulans by using a trpC plasmid.

1984 • 773 citations

Transformation by integration in Aspergillus nidulans

1983 • 655 citations

Selection for loss of tetracycline resistance by Escherichia coli

1981 • 585 citations

Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker.

1986 • 518 citations

Practical Methods in Molecular Biology

1981 • 497 citations

Intron splicing: a conserved internal signal in introns of animal pre-mRNAs.

1984 • 342 citations

Structure and function of the yeast URA3 gene: expression in Escherichia coli

1984 • 311 citations

Development of a high-frequency transforming vector for Aspergillus nidulans

1985 • 309 citations

Molecular analysis of a cell lineage

1983 • 286 citations

Transformation of Aspergillus nidulans by the orotidine-5′-phosphate decarboxylase gene of Neurospora crassa

1983 • 276 citations

Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli.

1979 • 247 citations

Construction and characterization of new cloning vehicles VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication

1982 • 212 citations

Primary structure of the trpC gene from Aspergillus nidulans

1985 • 206 citations

Cloning an Aspergillus nidulans developmental gene by transformation.

1985 • 198 citations

Strategies and Applications of in Vitro Mutagenesis

1985 • 192 citations

Direct and indirect gene replacements in Aspergillus nidulans.

1985 • 183 citations

Practical Methods in Molecular Biology.

1982 • 174 citations

Properties of Yeast Transformation

1979 • 173 citations

Cloning of the riboB locus of Aspergillus nidulans

1987 • 146 citations

A cosmid for selecting genes by complementation in Aspergillus nidulans : Selection of the developmentally regulated yA locus

1985 • 146 citations

Identification and functional analysis of beta-tubulin genes by site specific integrative transformation in Aspergillus nidulans.

1985 • 125 citations

Nucleotide sequence of the triosephosphate isomerase gene from Aspergillus nidulans: Implications for a differential loss of introns

1986 • 82 citations

Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulans

1983 • 81 citations

Identification and molecular analysis of a third Aspergillus nidulans alcohol dehydrogenase gene.

1985 • 80 citations

Cloning of the structural gene for orotidine 5′-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli

1983 • 75 citations

Molecular analysis of the argB gene of Aspergillus nidulans

1986 • 74 citations

Transcription and processing signals in the 3-phosphoglycerate kinase (PGK) gene from Aspergillus nidulans

1986 • 64 citations

A rapid method for preparation of bacterial plasmids

1983 • 53 citations

Sequence analysis of the pyr-4 (orotidine 5'-P decarboxylase) gene of Neurospora crassa

1986 • 50 citations

Genetic and molecular characterization of argB+ transformants of Aspergillus nidulans

1986 • 50 citations

Isolation and characterization of the orotidine 5‘-monophosphate decarboxylase domain of the multifunctional protein uridine 5‘-monophosphate synthase.

1985 • 36 citations

Pyrimidine biosynthesis in Aspergillus nidulans

1975 • 35 citations

Cloning and physical analysis of the pyrF gene (coding for orotidine-5′-phosphate decarboxylase) from Escherichia coli K-12

1983 • 25 citations

Isolation and characterization of cold-sensitive mutations at the benA, β-tubulin, locus of Aspergillus nidulans

1985 • 22 citations

Chapter 6 Molecular and Genetic Methods for Studying Mitosis and Spindle Proteins in Aspergillus nidulans

1982 • 13 citations

Cloning of Aspergillus nidulans DNA. I. Selection and analysis of recombinant plasmids capable of complementing pyrF, argIF and proAB mutations in Escherichia coli.

1980 • 3 citations