Characterization of Two Related Drosophila γ-tubulin Complexes that Differ in Their Ability to Nucleate Microtubules

Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases

1966 • 2,239 citations

Nucleation of microtubule assembly by a γ-tubulin-containing ring complex

1995 • 851 citations

Identification of γ-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans

1989 • 613 citations

γ-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans

1990 • 558 citations

Microtubule nucleation by γ-tubulin-containing rings in the centrosome

1995 • 530 citations

The Human Homologue of Bub3 Is Required for Kinetochore Localization of Bub1 and a Mad3/Bub1-related Protein Kinase

1998 • 460 citations

In vitro reconstitution of centrosome assembly and function: The central role of γ-tubulin

1994 • 457 citations

γ-Tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation

1992 • 447 citations

Role of GTP hydrolysis in microtubule dynamics: information from a slowly hydrolyzable analogue, GMPCPP.

1992 • 376 citations

The fission yeast γ-tubulin is essential for mitosis and is localized at microtubule organizing centers

1991 • 294 citations

Tubulin-nucleotide interactions during the polymerization and depolymerization of microtubules

1976 • 281 citations

Cytoplasmic microtubules in tissue culture cells appear to grow from an organizing structure towards the plasma membrane.

1976 • 263 citations

Spc98p and Spc97p of the yeast gamma -tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p

1997 • 239 citations

Recruitment of the γ-Tubulin Ring Complex to Drosophila Salt-stripped Centrosome Scaffolds

1998 • 233 citations

Centrosome assembly in vitro: role of gamma-tubulin recruitment in Xenopus sperm aster formation

1994 • 228 citations

Three-dimensional structural characterization of centrosomes from early Drosophila embryos.

1995 • 212 citations

The Mammalian γ-Tubulin Complex Contains Homologues of the Yeast Spindle Pole Body Components Spc97p and Spc98p

1998 • 197 citations

The spindle pole body component Spc97p interacts with the gamma -tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication

1997 • 193 citations

Polarity and nucleation of microtubules in polarized epithelial cells

1995 • 192 citations

The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment.

1996 • 183 citations

Protofilaments and rings, two conformations of the tubulin family conserved from bacterial FtsZ to alpha/beta and gamma tubulin.

1996 • 179 citations

Gamma-tubulin is required for the structure and function of the microtubule organizing centre in Drosophila neuroblasts.

1995 • 179 citations

The kinetics of microtubule assembly. Evidence for a two-stage nucleation mechanism.

1984 • 179 citations

Receptors determine the cellular localization of a γ-tubulin complex and thereby the site of microtubule formation

1998 • 168 citations

A highly divergent gamma-tubulin gene is essential for cell growth and proper microtubule organization in Saccharomyces cerevisiae.

1995 • 157 citations

Analysis of Tub4p, a yeast gamma-tubulin-like protein: implications for microtubule-organizing center function.

1996 • 156 citations

gamma-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation.

1996 • 150 citations

Microtubule organizing centers and γ-tubulin

1994 • 140 citations

Characterization of the Human Homologue of the Yeast Spc98p and Its Association with γ-Tubulin

1998 • 123 citations

Xgrip109: A γ Tubulin–Associated Protein with an Essential Role in γ Tubulin Ring Complex (γTuRC) Assembly and Centrosome Function

1998 • 112 citations

Centriole distribution during tripolar mitosis in Chinese hamster ovary cells.

1984 • 112 citations

Centrosome-microtubule nucleation

1997 • 100 citations

Cryoelectron Microscopy of Microtubules

1993 • 97 citations

Centrosomes Isolated from Spisula solidissima Oocytes Contain Rings and an Unusual Stoichiometric Ratio of α/β Tubulin

1997 • 97 citations

Purification of a multiprotein complex containing centrosomal proteins from the Drosophila embryo by chromatography with low-affinity polyclonal antibodies.

1992 • 92 citations

Chapter 25 Immunofluorescence Analysis of the Cytoskeleton during Oogenesis and Early Embryogenesis

1994 • 86 citations

Organization and energy-dependent growth of microtubules in cells.

1976 • 82 citations

Determination of free and bound microtubular protein and guanine nucleotide under equilibrium conditions

1979 • 77 citations

Spontaneous nucleation of microtubules

1995 • 66 citations

Analysis of the γ-tubulin sequences: Implications for the functional properties of γ-tubulin

1995 • 62 citations

The bulk of unpolymerized actin in Xenopus egg extracts is ATP-bound.

1995 • 58 citations

The role of γ-tubulin in mitotic spindle formation and cell cycle progression in Aspergillus nidulans

1997 • 52 citations

Protein complexes containing gamma-tubulin are present in mammalian brain microtubule protein preparations

1997 • 49 citations

[41] Purification of cytoskeletal proteins using peptide antibodies

1998 • 32 citations

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