Escherichia coli strain for thermoinducible T7 RNA polymerase-driven expression

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection.

1987 • 1,190 citations

Lambdoid phages that simplify the recovery of in vitro recombinants

1977 • 779 citations

A versatile method for integration of genes and gene fusions into the λ attachment site of Escherichia coli

1991 • 52 citations

Lys631 residue in the active site of the bacteriophage T7 RNA polymerase

1991 • 27 citations

A versatile and highly repressible Escherichia coli expression system based on invertible promoters: expression of a gene encoding a toxic product

1993 • 19 citations