Isolation and Characterization of cDNAs Corresponding to Two Human Calcium, Calmodulin-regulated, 3′,5′-Cyclic Nucleotide Phosphodiesterases

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

1988 • 2,903 citations

RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression

1987 • 2,333 citations

High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier

1989 • 2,140 citations

Yeast transformation: a model system for the study of recombination.

1981 • 1,491 citations

Primary sequence of cyclic nucleotide phosphodiesterase isozymes and the design of selective inhibitors

1990 • 860 citations

A simple and efficient procedure for transformation of yeasts.

1992 • 637 citations

Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene

1980 • 511 citations

Molecular analysis of the para locus, a sodium channel gene in Drosophila

1989 • 501 citations

PROTEINASE MUTANTS OF SACCHAROMYCES CEREVISIAE

1977 • 460 citations

PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors.

1986 • 400 citations

Cloning and characterization of four SIR genes of Saccharomyces cerevisiae.

1986 • 305 citations

The identification of a new cyclic nucleotide phosphodiesterase activity in human and guinea-pig cardiac ventricle. Implications for the mechanism of action of selective phosphodiesterase inhibitors

1987 • 303 citations

Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae.

1986 • 295 citations

Nucleotide sequence and transcriptional mapping of the yeastpet56-his3-dedlgene region

1985 • 290 citations

A Family of Human Phosphodiesterases Homologous to the Dunce Learning and Memory Gene Product of Drosophila melanogaster are Potential Targets for Antidepressant Drugs

1993 • 287 citations

Multiple molecular forms of cyclic nucleotide phosphodiesterase in cardiac and smooth muscle and in platelets

1986 • 249 citations

A family of human phosphodiesterases homologous to the dunce learning and memory gene product of Drosophila melanogaster are potential targets for antidepressant drugs.

1993 • 233 citations

Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae.

1987 • 221 citations

Isolation and characterization of a previously undetected human cAMP phosphodiesterase by complementation of cAMP phosphodiesterase-deficient Saccharomyces cerevisiae

1993 • 219 citations

Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase.

1989 • 192 citations

Molecular cloning and characterization of a calmodulin-dependent phosphodiesterase enriched in olfactory sensory neurons.

1995 • 190 citations

Calculation of the isoelectric points of polypeptides from the amino acid composition

1986 • 185 citations

Calcium/calmodulin-activated phosphodiesterase expressed in olfactory receptor neurons

1992 • 164 citations

[37] Recognition and characterization of calmodulin-binding sequences in peptides and proteins

1987 • 143 citations

Effects of vinpocetine on cyclic nucleotide metabolism in vascular smooth muscle

1984 • 138 citations

Molecular cloning of a cDNA encoding the “61-kDa” calmodulin-stimulated cyclic nucleotide phosphodiesterase. Tissue-specific expression of structurally related isoforms.

1993 • 99 citations

Evidence for domain organization within the 61-kDa calmodulin-dependent cyclic nucleotide phosphodiesterase from bovine brain

1991 • 85 citations

Molecular cloning of cDNA encoding a “63”-kDa calmodulin-stimulated phosphodiesterase from bovine brain.

1992 • 72 citations

[46] Methylxanthine inhibitors of phosphodiesterases

1988 • 66 citations

A polymerase chain reaction strategy to identify and clone cyclic nucleotide phosphodiesterase cDNAs. Molecular cloning of the cDNA encoding the 63-kDa calmodulin-dependent phosphodiesterase.

1992 • 65 citations

Proteolytic activation of calmodulin-dependent cyclic nucleotide phosphodiesterase.

1985 • 64 citations

Molecular cloning of DNA encoding a calmodulin-dependent phosphodiesterase enriched in striatum.

1992 • 63 citations

Phosphorylation of the 61-kDa Calmodulin-Stimulated Cyclic Nucleotide Phosphodiesterase at Serine 120 Reduces Its Affinity for Calmodulin

1994 • 58 citations

Characterization of a calcium-calmodulin-stimulated cyclic GMP phosphodiesterase from bovine brain

1985 • 51 citations

Sequence comparison of the 63-, 61-, and 59-kDa calmodulin-dependent cyclic nucleotide phosphodiesterases

1991 • 45 citations

Characterization of calmodulin-dependent cyclic nucleotide phosphodiesterase isoenzymes

1994 • 44 citations

Phenotypic analysis of proteinase A mutants. Implications for autoactivation and the maturation pathway of the vacuolar hydrolases of Saccharomyces cerevisiae.

1993 • 44 citations

Testis-specific calmodulin-dependent phosphodiesterase. A distinct high affinity cAMP isoenzyme immunologically related to brain calmodulin-dependent cGMP phosphodiesterase.

1988 • 37 citations

Calmodulin-dependent cyclic nucleotide phosphodiesterases in the immature rat testis.

1981 • 33 citations

Characterization of a calmodulin-dependent high-affinity cyclic AMP and cyclic GMP phosphodiesterase from male mouse germ cells

1984 • 26 citations

Purification and kinetic properties of two soluble forms of calmodulin-dependent cyclic nucleotide phosphodiesterase from rat pancreas

1983 • 26 citations

Substituted carbostyrils as inhibitors of cyclic AMP phosphodiesterase

1985 • 18 citations

Separation and Assay of Phosphodiesterase Isoforms in Murine Peritoneal Macrophages Using Membrane Matrix DEAE Chromatography and [32P]cAMP

1993 • 9 citations