Circular dichroism, Raman spectroscopy, and gel filtration of trapped folding intermediates of ribonuclease

Principles that Govern the Folding of Protein Chains

1973 • 6,771 citations

A thermodynamic approach to the problem of stabilization of globular protein structure: A calorimetric study

1974 • 1,384 citations

THE KINETICS OF FORMATION OF NATIVE RIBONUCLEASE DURING OXIDATION OF THE REDUCED POLYPEPTIDE CHAIN

1961 • 1,148 citations

Consideration of the possibility that the slow step in protein denaturation reactions is due to cis-trans isomerism of proline residues

1975 • 1,108 citations

Interpretation of the doublet at 850 and 830 cm^-1 in the Raman spectra of tyrosyl residues in proteins and certain model compounds

1975 • 564 citations

Experimental studies of protein folding and unfolding

1979 • 535 citations

24 Bovine Pancreatic Ribonuclease

1971 • 437 citations

Thermodynamics of protein denaturation. III. Denaturation of ribonuclease in water and in aqueous urea and aqueous ethanol mixtures

1967 • 422 citations

Circular Dichroism of Biological Macromolecules

1966 • 365 citations

Thermodynamics of protein denaturation. Effect of pressure on the denaturation on ribonuclease A

1970 • 301 citations

Regeneration of Native Secondary and Tertiary Structures by Air Oxidation of Reduced Ribonuclease

1961 • 280 citations

Reductive Cleavage of Disulfide Bridges in Ribonuclease

1957 • 278 citations

Structural Studies of Ribonuclease. V. Reversible Change of Configuration^1-3

1961 • 263 citations

Intermediates in Protein Folding Reactions and the Mechanism of Protein Folding

1975 • 257 citations

Aromatic side‐chain contributions to the far ultraviolet circular dichroism of peptides and proteins

1978 • 245 citations

Acid catalysis of the formation of the slow-folding species of RNase A: Evidence that the reaction is proline isomerization

1978 • 229 citations

Side-chain Interactions Governing the Pairing of Half-cystine Residues in Ribonuclease

1962 • 227 citations

Laser-excited Raman spectroscopy of biomolecules

1970 • 197 citations

A quantitative treatment of the kinetics of the folding transition of ribonuclease A

1976 • 167 citations

Role of proline isomerization in folding of ribonuclease A at low temperatures

1979 • 161 citations

Nonenzymic reactivation of reduced bovine pancreatic ribonuclease by air oxidation and by glutathione oxidoreduction buffers.

1975 • 159 citations

Intermediates in the refolding of reduced ribonuclease A

1979 • 159 citations

Both the Fast and Slow Refolding Reactions of Ribonuclease A Yield Native Enzyme

1973 • 157 citations

Protein-solvent interactions and protein conformation

1970 • 146 citations

Test of the extended two-state model for the kinetic intermediates observed in the folding transition of ribonuclease A

1978 • 137 citations

Pathways of folding of reduced bovine pancreatic ribonuclease

1974 • 129 citations

The two-disulphide intermediates and the folding pathway of reduced pancreatic trypsin inhibitor

1975 • 126 citations

Kinetics of refolding of reduced ribonuclease

1977 • 125 citations

Some Specific Ion Effects on the Conformation and Thermal Stability of Ribonuclease^*

1965 • 121 citations

Laser-excited Raman spectroscopy of biomolecules. 9. Laser Raman spectroscopic studies of the thermal unfolding of ribonuclease A

1976 • 111 citations

Structural Studies of Ribonuclease. VII. Chymotryptic Hydrolysis of Ribonuclease A at Elevated Temperatures^*

1963 • 97 citations

Thermodynamics of the denaturation of ribonuclease by guanidine hydrochloride

1970 • 93 citations

The formation of the tertiary structure of proteins.

1967 • 90 citations

Unfolding and refolding occur much faster for a proline-free proteins than for most proline-containing proteins.

1977 • 88 citations

A hypothesis for the pathway of the thermally-induced unfolding of bovine pancreatic ribonuclease

1975 • 82 citations

Guanidine-unfolded state of ribonuclease A contains both fast- and slow-refolding species.

1976 • 82 citations

Circular Dichroism of Ribonuclease A, Ribonuclease S, and Some Fragments

1968 • 72 citations

Further evidence suggesting that the slow phase in protein unfolding and refolding is due to proline isomerization: a kinetic study of carp parvalbumins

1978 • 68 citations

The heat-unfolded state of ribonuclease A is an equilibrium mixture of fast and slow refolding species

1975 • 67 citations

The conformation fo poly‐<scp>L</scp>‐alanine in hexafluoroisopropanol

1972 • 66 citations

Spectroscopic studies of random chain and α‐helical polypeptides in hexafluoroisopropanol

1971 • 64 citations

A study of renaturation of reduced hen egg white lysozyme. Enzymically active intermediates formed during oxidation of the reduced protein.

1976 • 60 citations

Immunochemical analysis of the conformational properties of intermediates trapped in the folding and unfolding of bovine pancreatic trypsin inhibitor

1978 • 59 citations

Nuclear magnetic resonance studies of the unfolding of pancreatic ribonuclease

1975 • 56 citations

Interactions contributing to the tyrosyl circular dichroism bands of ribonuclease S and A

1972 • 55 citations

Optical Activity of Cystine-containing Proteins

1969 • 53 citations

Salt effects in the glutathione-facilitated reactivation of reduced bovine pancreatic ribonuclease.

1975 • 50 citations

Immunological determination of the order of folding of portions of the molecule during air oxidation of reduced ribonuclease

1977 • 49 citations

Regeneration of ribonuclease A from the reduced protein. 1. Conformational analysis of the intermediates by measurements of enzymic activity, optical density, and optical rotation

1980 • 48 citations

Nuclear Magnetic Resonance Study of the Thermal Denaturation of Ribonuclease A: Implications for Multistate Behavior at Low pH

1973 • 47 citations

Steps in the pathway of the thermal unfolding of ribonuclease A. A nonspecific photochemical surface-labeling study

1979 • 46 citations

Thermal Effects on the Circular Dichroism Spectra of Ribonuclease A and of Ribonuclease S-Protein

1969 • 45 citations

Immobilized carboxypeptidase A as a probe for studying the thermally induced unfolding of bovine pancreatic ribonuclease

1975 • 43 citations

Folding of ribonuclease, S-protein, and des(121-124)-ribonuclease during glutathione oxidation of the reduced proteins

1980 • 43 citations

CALORIMETRIC INVESTIGATION OF RIBONUCLEASE THERMAL DENATURATION

1973 • 39 citations

Nuclear magnetic resonance studies of the unfolding of pancreatic ribonuclease

1975 • 37 citations

Ultraviolet difference spectroscopy of intermediates trapped in unfolding and refolding of bovine pancreatic trypsin inhibitor

1980 • 35 citations

A three‐disulphide intermediate in refolding of reduced ribonuclease a with a folded conformation

1980 • 34 citations

A Circular Dichroic Spectral Study on Disuffide-Reduced Pancreatic Ribonuclease A and Its Renaturation to the Active Enzyme1

1977 • 33 citations

The Reoxidation of Reduced Ribonuclease Derivatives

1964 • 31 citations

The thermal unfolding of ribonuclease A A 13C NMR study

1979 • 29 citations

Early steps in the refolding reaction of reduced ribonuclease A

1978 • 29 citations

Formation of the four isomers of hen egg white lysozyme containing three native disulfide bonds and one open disulfide bond

1977 • 28 citations

Steady-state kinetic study of action of ribonuclease A, involving a conformational change between 30 and 40.degree.C

1979 • 22 citations

Electron paramagnetic resonance spectroscopy as a monitor of the pathway of the thermal unfolding of ribonuclease A

1977 • 21 citations

MECHANISM OF GLUTATHIONE REGENERATION OF REDUCED PANCREATIC RIBONUCLEASE A

1975 • 19 citations

Molecular Sieve Studies of Interacting Protein Systems

1974 • 19 citations

Thermal unfolding transition of ribonuclease A measured by 2'-CMP binding

1977 • 17 citations

Elongation of the Disulfide Bonds of Bovine Pancreatic Ribonuclease and the Effect of the Modification on the Properties of the Enzyme

1971 • 11 citations

The Reoxidation of Reduced Ribonuclease to the Native Conformation as Determined by Circular Dichroism

1968 • 9 citations