Homologous recombination in the nuclear genome of Chlamydomonas reinhardtii.

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Genomic sequencing.

1984 • 8,343 citations

A rapid boiling method for the preparation of bacterial plasmids

1981 • 3,334 citations

High efficiency transformation of E.coli by high voltage electroporation

1988 • 2,789 citations

Transformation of yeast.

1978 • 2,267 citations

Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice

1991 • 2,028 citations

Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes

1988 • 1,690 citations

Yeast transformation: a model system for the study of recombination.

1981 • 1,491 citations

Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells

1979 • 1,454 citations

Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1

1991 • 1,321 citations

High-frequency nuclear transformation of Chlamydomonas reinhardtii.

1990 • 1,047 citations

Disruption of the Dictyostelium Myosin Heavy Chain Gene by Homologous Recombination

1987 • 984 citations

Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination

1985 • 978 citations

Chloroplast Transformation in Chlamydomonas with High Velocity Microprojectiles

1988 • 957 citations

[36] Optimizing the biolistic process for different biological applications

1993 • 649 citations

High frequency targeting of genes to specific sites in the mammalian genome

1986 • 619 citations

Patterns of Integration of DNA Microinjected into Cultured Mammalian Cells: Evidence for Homologous Recombination Between Injected Plasmid DNA Molecules

1982 • 417 citations

The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus.

1989 • 404 citations

Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase.

1989 • 382 citations

Gene targeting in plants

1988 • 370 citations

Mitochondrial Transformation in Yeast by Bombardment with Microprojectiles

1988 • 314 citations

Germ-Line Transmission of a c- abl Mutation Produced by Targeted Gene Disruption in ES Cells

1989 • 285 citations

Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii.

1989 • 239 citations

Extrachromosomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation.

1990 • 217 citations

A simple procedure for maximum yield of high-quality plasmid DNA.

1990 • 181 citations

Recombination During Gene Transfer into Mouse Cells Can Restore the Function of Deleted Genes

1983 • 148 citations

High-Efficiency Ligation and Recombination of DNA Fragments by Vertebrate Cells

1983 • 148 citations

Homologous recombination in plant cells after Agrobacterium-mediated transformation.

1990 • 139 citations

Homologous Recombination and Stable Transfection in the Parasitic Protozoan Trypanosoma brucei

1990 • 135 citations

Stable nuclear transformation of Chlamydomonas reinhardtii by using a C. reinhardtii gene as the selectable marker.

1990 • 129 citations

Homologous recombination in mammalian cells mediates formation of a functional gene from two overlapping gene fragments.

1983 • 129 citations

Gene targeting in normal and amplified cell lines

1990 • 116 citations