Use of the yeast one-hybrid system to screen for mutations in the ligand-binding domain of the estrogen receptor

THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS

1949 • 19,979 citations

The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases

1993 • 5,680 citations

MOLECULAR MECHANISMS OF ACTION OF STEROID/THYROID RECEPTOR SUPERFAMILY MEMBERS

1994 • 2,875 citations

Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A

1986 • 2,341 citations

The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.

1993 • 1,459 citations

The estrogen receptor binds tightly to its responsive element as a ligand-induced homodimer

1988 • 1,233 citations

The human estrogen receptor has two independent nonacidic transcriptional activation functions

1989 • 1,066 citations

Identification of a conserved region required for hormone dependent transcriptional activation by steroid hormone receptors.

1992 • 829 citations

Role of the two activating domains of the oestrogen receptor in the cell-type and promoter-context dependent agonistic activity of the anti-oestrogen 4-hydroxytamoxifen.

1990 • 772 citations

The chicken oestrogen receptor sequence: homology with v-erbA and the human oestrogen and glucocorticoid receptors.

1986 • 733 citations

Characterization and colocalization of steroid binding and dimerization activities in the mouse estrogen receptor

1990 • 617 citations

The hormone-binding domains of the estrogen and glucocorticoid receptors contain an inducible transcription activation function

1988 • 588 citations

Molecular cloning and charcterization of rat estrogen receptor cDNA

1987 • 488 citations

The cloned human oestrogen receptor contains a mutation which alters its hormone binding properties.

1989 • 428 citations

Isolation of ORC6 , a Component of the Yeast Origin Recognition Complex by a One-Hybrid System

1993 • 416 citations

Structural Organization and Expression of the Mouse Estrogen Receptor

1987 • 365 citations

Characterization of the Amino-terminal Transcriptional Activation Function of the Human Estrogen Receptor in Animal and Yeast Cells

1995 • 230 citations

Identification of residues in the estrogen receptor that confer differential sensitivity to estrogen and hydroxytamoxifen.

1993 • 215 citations

Structure-function analysis of the hormone binding domain of the human estrogen receptor by region-specific mutagenesis and phenotypic screening in yeast.

1993 • 186 citations

TheXenopus laevisEstrogen Receptor: Sequence Homology with Human and Avian Receptors and Identification of Multiple Estrogen Receptor Messenger Ribonucleic Acids*

1987 • 159 citations

A reliable method for random mutagenesis: the generation of mutant libraries using spiked oligodeoxyribonucleotide primers

1989 • 135 citations

Differential DNA-binding abilites of estrogen receptor occupied with two classes of antiestrogens: studies using human estrogen receptor overexpressed in mammalian cells

1991 • 128 citations

Human estrogen receptor mutants with altered estrogen and antiestrogen ligand discrimination.

1992 • 126 citations

Identification of Cysteine 530 as the Covalent Attachment Site of an Affinity-labeling Estrogen (Ketononestrol Aziridine) and Antiestrogen (Tamoxifen Aziridine) in the Human Estrogen Receptor

1989 • 121 citations

Full-length sequence and in vitro expression of rainbow trout estrogen receptor cDNA

1990 • 119 citations

Promoter specificity of the two transcriptional activation functions of the human oestrogen receptor in yeast

1992 • 103 citations

Identification of residues in the estrogen receptor that confer differential sensitivity to estrogen and hydroxytamoxifen

1993 • 92 citations

A highly conserved region in the hormone-binding domain of the human estrogen receptor functions as an efficient transactivation domain in yeast

1994 • 72 citations

Structural requirements for high affinity ligand binding by estrogen receptors: a comparative analysis of truncated and full length estrogen receptors expressed in bacteria, yeast, and mammalian cells.

1992 • 51 citations

Identification of two cysteines closely positioned in the ligand-binding pocket of the human estrogen receptor: roles in ligand binding and transcriptional activation.

1992 • 41 citations

Structural requirements for high affinity ligand binding by estrogen receptors: a comparative analysis of truncated and full length estrogen receptors expressed in bacteria, yeast, and mammalian cells

1992 • 20 citations

Identification of two cysteines closely positioned in the ligand- binding pocket of the human estrogen receptor: roles in ligand binding and transcriptional activation

1992 • 20 citations