Site-directed Mutagenesis of Human Glutathione Transferase P1-1

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Tissue sulfhydryl groups

1959 • 25,130 citations

MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures

1991 • 14,222 citations

On the nature of allosteric transitions: A plausible model

1965 • 8,699 citations

Comparison of Experimental Binding Data and Theoretical Models in Proteins Containing Subunits^*

1966 • 2,956 citations

[51] Assays for differentiation of glutathione S-Transferases

1981 • 2,259 citations

Glutathione Transferases—Structure and Catalytic Activit

1988 • 1,751 citations

Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties.

1985 • 1,081 citations

The Isoenzymes of Glutathione Transferase

1985 • 960 citations

Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea

1986 • 926 citations

Overexpression of a novel anionic glutathione transferase in multidrug-resistant human breast cancer cells.

1986 • 791 citations

Theta, a new class of glutathione transferases purified from rat and man

1991 • 743 citations

Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

1977 • 711 citations

The Glutathione S ‐Transferases: A Group of Multifunctional Detoxification Proteins

1978 • 686 citations

Structure and function of glutathione S-transferases

1994 • 638 citations

GLUTATHIONE S-TRANSFERASES: GENE STRUCTURE, REGULATION, AND BIOLOGICAL FUNCTION

1989 • 624 citations

Purification of glutathione S-transferases from human liver by glutathione-affinity chromatography

1977 • 481 citations

Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli

1984 • 468 citations

Structure Determination and Refinement of Human Alpha Class Glutathione Transferase A1-1, and a Comparison with the Mu and Pi Class Enzymes

1993 • 449 citations

X‐ray crystal structures of cytosolic glutathione S‐transferases

1994 • 430 citations

Aspartate Transcarbamylase

1971 • 422 citations

The three-dimensional structure of a glutathione S-transferase from the Mu gene class. Structural analysis of the binary complex of isoenzyme 3-3 and glutathione at 2.2-.ANG. resolution

1992 • 372 citations

The three-dimensional structure of class pi glutathione S-transferase in complex with glutathione sulfonate at 2.3 A resolution.

1991 • 356 citations

Glutathione S-transferases: reaction mechanism, structure, and function

1991 • 334 citations

Three-dimensional structure of class π glutathione S-transferase from human placenta in complex with S-hexylglutathione at 2.8 Å resolution

1992 • 277 citations

Glutathione transferases: Nomenclature

1984 • 231 citations

Structure and expression of a human class pi glutathione S-transferase messenger RNA.

1987 • 213 citations

Expression of recombinant glutathione S-transferase pi, Ya, or Yb1 confers resistance to alkylating agents.

1990 • 148 citations

Kinetic independence of the subunits of cytosolic glutathione transferase from the rat

1985 • 136 citations

Expression of human glutathione S-transferases in Saccharomyces cerevisiae confers resistance to the anticancer drugs adriamycin and chlorambucil

1990 • 130 citations

Three-dimensional Structure of the Glutathione Synthetase from Escherichia coli B at 2·0 Å Resolution

1993 • 121 citations

Quantification of human hepatic glutathione S-transferases

1990 • 120 citations

Elevation of the placental glutathione S-transferase form (GST-pi) in tumor tissues and the levels in sera of patients with cancer.

1989 • 106 citations

Specific inactivation of glutathione S-transferases in Class Pi by SH-modifiers

1990 • 102 citations

Redox forms of human placenta glutathione transferase.

1991 • 88 citations

Site-directed Mutagenesis of Human Glutathione Transferase P1-1

1995 • 82 citations

Protein expression using cotranslational fusion and cleavage of ubiquitin. Mutagenesis of the glutathione-binding site of human Pi class glutathione S-transferase.

1994 • 73 citations

Peculiar spectroscopic and kinetic properties of Cys-47 in human placental glutathione transferase. Evidence for an atypical thiolate ion pair near the active site.

1993 • 71 citations

Three residues involved in binding and catalysis in the carbamyl phosphate binding site of Escherichia coli aspartate transcarbamylase

1989 • 67 citations

The binding of substrates and a product of the enzymatic reaction to glutathione S-transferase A.

1979 • 67 citations

Contribution of five amino acid residues in the glutathione-binding site to the function of human glutathione transferase P1-1

1992 • 65 citations

Expression of human glutathione S-transferase 2 in Escherichia coli. Immunological comparison with the basic glutathione S-transferases isoenzymes from human liver

1987 • 63 citations

Role of cysteine residues in the activity of rat glutathione transferase P (7-7): Elucidation by oligonucleotide site-directed mutagenesis

1991 • 59 citations

Mutational substitution of residues implicated by crystal structure in binding the substrate glutathione to human glutathione S-transferase π

1992 • 59 citations

Kinetic studies and active site-binding properties of glutathione S-transferase using spin-labeled glutathione, a product analogue.

1984 • 57 citations

Identification of a highly reactive sulphydryl group in human placental glutathione transferase by a site‐directed fluorescent reagent

1990 • 52 citations

Triggering of Allostery in an Enzyme by a Point Mutation: Ornithine Transcarbamoylase

1989 • 52 citations

A rapid equilibrium random sequential bi-bi mechanism for human placental glutathione S-transferase

1989 • 48 citations

Cooperativity Induced by a Single Mutation at the Subunit Interface of a Dimeric Enzyme: Glutathione Reductase

1992 • 45 citations

Non-essentiality of cysteine and histidine residues for the activity of human class Pi glutathione S-transferase

1991 • 42 citations

Nonequivalence of the Two Subunits of Horse Erythrocyte Glutathione Transferase in Their Reaction with Sulfhydryl Reagents

1989 • 40 citations

Site-directed mutagenesis study on the roles of evolutionally conserved aspartic acid residues in human glutathione S-transferase P1-1

1993 • 39 citations

Purification and bilirubin binding properties of glutathione S‐transferase from human placenta

1981 • 38 citations

Mutation of lysine 233 to alanine introduces positive cooperativity into tyrosyl-tRNA synthetase

1993 • 38 citations

Inactivation of mouse liver glutathione S-transferase YfYf (Pi class) by ethacrynic acid and 5,5′-dithiobis-(2-nitrobenzoic acid)

1993 • 35 citations

Explanation of the non-hyperbolic kinetics of the glutathione S-transferases by the simplest steady-state random sequential Bi Bi mechanism

1990 • 33 citations

Conformational States of Human Placental Glutathione Transferase as Probed by Limited Proteolysis

1993 • 31 citations

Conversion of the noncooperative Bacillus subtilis aspartate transcarbamoylase into a cooperative enzyme by a single amino acid substitution

1992 • 29 citations

Interaction of glutathione transferase from horse erythrocytes with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole

1991 • 26 citations

Site-Directed Mutagenesis of Amino Acid Residues Involved in the Glutathione Binding of Human Glutathione S-Transferase P1-1

1992 • 21 citations

Evidence against a relationship between fatty acid ethyl ester synthase and the Pi class glutathione S-transferase in humans

1993 • 19 citations

Intrinsic fluorescence quenching of glutathione transferase pi by glutathione binding.

1991 • 8 citations