Heterologous expression of G-protein-coupled receptors

Molecular characterization of a peripheral receptor for cannabinoids

1993 • 4,950 citations

Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation

1991 • 2,981 citations

Structure and Functional Expression of a Human Interleukin-8 Receptor

1991 • 1,088 citations

STRUCTURE AND FUNCTION OF G PROTEIN-COUPLED RECEPTORS

1994 • 1,070 citations

The probable arrangement of the helices in G protein-coupled receptors.

1993 • 977 citations

Palmitoylation of the human β2-adrenergic receptor

1989 • 599 citations

Molecular Cloning of the Thyrotropin Receptor

1989 • 529 citations

Physiological effects of inverse agonists in transgenic mice with myocardial overexpression of the β2-adrenoceptor

1995 • 408 citations

Overexpression of integral membrane proteins for structural studies

1995 • 388 citations

Diversity and Selectivity of Receptor-G Protein Interaction

1996 • 365 citations

Inverse agonist activity of beta-adrenergic antagonists.

1994 • 357 citations

Assembly of functional rhodopsin requires a disulfide bond between cysteine residues 110 and 187.

1990 • 320 citations

Simultaneous coupling of alpha 2-adrenergic receptors to two G-proteins with opposing effects. Subtype-selective coupling of alpha 2C10, alpha 2C4, and alpha 2C2 adrenergic receptors to Gi and Gs.

1992 • 319 citations

Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells.

1991 • 283 citations

Locating ligand-binding sites in 7tm receptors by protein engineering

1994 • 282 citations

Fluorescent Labeling of Purified β2 Adrenergic Receptor

1995 • 271 citations

Control of Yeast Mating Signal Transduction by a Mammalian β ₂ -adrenergic Receptor and G _s α Subunit

1990 • 224 citations

Purification of a rat neurotensin receptor expressed in Escherichia coli

1996 • 203 citations

A Colorimetric Assay for Measuring Activation of Gs- and Gq-Coupled Signaling Pathways

1995 • 187 citations

Desensitization, phosphorylation and palmitoylation of the human dopamine D1 receptor

1994 • 176 citations

Agonist-modulated palmitoylation of beta 2-adrenergic receptor in Sf9 cells.

1992 • 176 citations

Expression of the Human Oxytocin Receptor in Baculovirus-Infected Insect Cells: High-Affinity Binding Is Induced by a Cholesterol-Cyclodextrin Complex

1995 • 162 citations

Expression of the Human 5-Hydroxytryptamine1A Receptor in Sf9 Cells

1995 • 151 citations

Molecular cloning and characterisation of the rat pituitary gonadotropin-releasing hormone (GnRH) receptor

1992 • 140 citations

Functional Coupling of a Mammalian Somatostatin Receptor to the Yeast Pheromone Response Pathway

1995 • 133 citations

Functional expression of the human serotonin 5-HT1A receptor in Escherichia coli. Ligand binding properties and interaction with recombinant G protein alpha-subunits.

1992 • 122 citations

Mutagenesis of N-Glycosylation Sites in the Human Vasoactive Intestinal Peptide 1 Receptor. Evidence That Asparagine 58 or 69 Is Crucial for Correct Delivery of the Receptor to Plasma Membrane

1996 • 101 citations

High‐level Expression of the Human Neurokinin‐1 Receptor in Mammalian Cell Lines using the Semliki Forest Virus Expression System

1994 • 98 citations

Expression of functional mouse 5‐HT_5A serotonin receptor in the methylotrophic yeast Pichia pastoris: pharmacological characterization and localization

1995 • 95 citations

Mediation of most atypical effects by species homologues of the β₃‐adrenoceptor

1994 • 91 citations

Molecular Mechanism of Action of Non-peptide Ligands for Peptide Receptors

1995 • 78 citations

Tools for investigating functional interactions between ligands and G-protein-coupled receptors

1994 • 76 citations

Differential regulation of cAMP-mediated gene transcription by m1 and m4 muscarinic acetylcholine receptors. Preferential coupling of m4 receptors to Gi alpha-2

1994 • 70 citations

Expression of the human D_2S dopamine receptor in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe: A comparative study

1994 • 59 citations

Differential coupling of rat D2 dopamine receptor isoforms expressed in Spodoptera frugiperda insect cells.

1996 • 49 citations

Heterologous Expression of the Human D_2S Dopamine Receptor in Protease‐Deficient Saccharomyces cerevisiae Strains

1994 • 46 citations

Are receptors promiscuous? Intrinsic efficacy as a transduction phenomenon

1988 • 43 citations

Functional Expression of Recombinant G-Protein-Coupled Receptors Monitored by Video Imaging of Pigment Movement in Melanophores

1993 • 42 citations

The use of the amplifiable high-expression vector pEE14 to study the interactions of autoantibodies with recombinant human thyrotrophin receptor

1992 • 41 citations

Stable overexpression of human ?2-adrenergic receptors in mammalian cells

1992 • 41 citations

Molecular Cloning and Pharmacological Characterization of the Bovine beta3-adrenergic Receptor

1995 • 34 citations

Use of a luciferase reporter system for characterizing G-protein-linked receptors

1995 • 33 citations

Co‐expression of the neurokinin NK2 receptor and G‐protein components in the fission yeast Schizosaccharomyces pombe

1995 • 24 citations

Expression and Solubilization of a Recombinant Human Neurokinin-1 Receptor in Insect Cells

1994 • 19 citations

Further Development of the Locus Control Region/Murine Erythroleukemia Expression System: High Level Expression and Characterization of Recombinant Human Calcitonin Receptor

1995 • 17 citations

Molecular Cloning and Pharmacological Characterization of the Bovine beta3-adrenergic Receptor

1995 • 17 citations

High level expression of human neuropeptide Y receptors in mammalian cells infected with a recombinant vaccinia virus

1993 • 16 citations

Similar ligand binding in recombinant human α2C2-adrenoceptors produced in mammalian, insect and yeast cells

1994 • 11 citations