Sequence requirements for membrane assembly of polytopic membrane proteins: molecular dissection of the membrane insertion process and topogenesis of the human MDR3 P-glycoprotein.

Intracellular protein topogenesis

1980 • 1,378 citations

How N-linked oligosaccharides affect glycoprotein folding in the endoplasmic reticulum.

1994 • 617 citations

Predicting the orientation of eukaryotic membrane-spanning proteins.

1989 • 596 citations

Genomic organization of the human multidrug resistance (MDR1) gene and origin of P-glycoproteins.

1990 • 271 citations

Membrane Topology of a Cysteine-less Mutant of Human P-glycoprotein

1995 • 252 citations

Characterization of the human MDR3 P-glycoprotein and its recognition by P-glycoprotein-specific monoclonal antibodies.

1991 • 212 citations

Bovine opsin has more than one signal sequence

1985 • 186 citations

Insertion of a multispanning membrane protein occurs sequentially and requires only one signal sequence

1988 • 185 citations

Identification of a translocated protein segment in a voltage-dependent channel

1994 • 158 citations

Transmembrane Organization of Mouse P-glycoprotein Determined by Epitope Insertion and Immunofluorescence

1996 • 142 citations

Evidence for an alternate model of human P-glycoprotein structure and biogenesis.

1993 • 138 citations

Study of membrane orientation and glycosylated extracellular loops of mouse P-glycoprotein by in vitro translation.

1991 • 132 citations

Multiple topogenic sequences in bovine opsin.

1987 • 115 citations

Membrane insertion of uracil permease, a polytopic yeast plasma membrane protein.

1991 • 98 citations

The erythrocyte anion transport protein is cotranslationally inserted into microsomes

1982 • 92 citations

Membrane Topology of P-Glycoprotein As Determined by Epitope Insertion: Transmembrane Organization of the N-Terminal Domain of mdr3

1995 • 86 citations

Amino-terminal assembly of human P-glycoprotein at the endoplasmic reticulum is directed by cooperative actions of two internal sequences.

1993 • 86 citations

The transmembrane topology of the amino terminus of the alpha subunit of the nicotinic acetylcholine receptor

1991 • 84 citations

Membrane topology of the N-terminal half of the hamster P-glycoprotein molecule

1993 • 82 citations

Biogenesis of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, an integral glycoprotein of the endoplasmic reticulum.

1984 • 80 citations

Structural requirements for membrane assembly of proteins spanning the membrane several times.

1989 • 79 citations

Genetic and biochemical evaluation of eucaryotic membrane protein topology: multiple transmembrane domains of Saccharomyces cerevisiae 3-hydroxy-3-methylglutaryl coenzyme A reductase.

1990 • 71 citations

Membrane topology of multidrug resistance protein expressed in Escherichia coli. N-terminal domain.

1994 • 67 citations

Transmembrane orientation and topogenesis of the third and fourth membrane-spanning regions of human P-glycoprotein (MDR1).

1994 • 49 citations

In vitro biosynthesis, core glycosylation and membrane integration of opsin

1981 • 49 citations

Topological Determinants of Internal Transmembrane Segments in P-glycoprotein Sequences

1995 • 44 citations

Multidrug Resistance Protein (Mdr)-alkaline Phosphatase Hybrids in Escherichia coli Suggest a Major Revision in the Topology of the C-terminal Half of Mdr

1995 • 43 citations

Membrane Insertion of Uracil Permease, a Poly topic Yeast Plasma Membrane Protein

1991 • 41 citations

Topological Folding and Proteolysis Profile of P-glycoprotein in Membranes of Multidrug-Resistant Cells: Implications for the Drug-Transport Mechanism

1996 • 40 citations

Translocation gets a push

1994 • 31 citations

Membrane insertion, processing, and topology of cystic fibrosis transmembrane conductance regulator (CFTR) in microsomal membranes

1996 • 28 citations

Genetic and Biochemical Evaluation of Eucaryotic Membrane Protein Topology: Multiple Transmembrane Domains of Saccharomyces cerevisiae 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase

1990 • 21 citations

Membrane orientation of transmembrane segments 11 and 12 of MDR- and non-MDR-associated P-glycoproteins

1993 • 17 citations