Theory and methods for immunization in culture and monoclonal antibody production

Continuous cultures of fused cells secreting antibody of predefined specificity

1975 • 17,037 citations

Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G

1971 • 3,630 citations

Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

1972 • 3,187 citations

Plaque Formation in Agar by Single Antibody-Producing Cells

1963 • 2,385 citations

Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed Recombinants

1964 • 2,108 citations

IMMUNIZATION OF DISSOCIATED SPLEEN CELL CULTURES FROM NORMAL MICE

1967 • 2,062 citations

A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines

1979 • 1,885 citations

Antibodies to major histocompatibility antigens produced by hybrid cell lines

1977 • 1,727 citations

A Theory of Self-Nonself Discrimination

1970 • 1,700 citations

A better cell line for making hybridomas secreting specific antibodies

1978 • 1,649 citations

Production of monoclonal antibodies: Strategy and tactics

1980 • 1,594 citations

Enzyme-Linked Immunosorbent Assay, Elisa

1972 • 1,489 citations

A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses

1975 • 788 citations

Selected methods in cellular immunology

1980 • 747 citations

Fusion between immunoglobulin‐secreting and nonsecreting myeloma cell lines

1976 • 729 citations

The Regulatory Influence of Activated T Cells on B Cell Responses to Antigen

1972 • 633 citations

Bacterial Endotoxins and Host Immune Responses

1980 • 629 citations

A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells

1977 • 615 citations

A Requirement for Two Cell Types for Antibody Formation in vitro

1967 • 612 citations

Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine

1963 • 611 citations

T Cell Control of Antibody Production

1974 • 582 citations

Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment

1975 • 549 citations

Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes.

1978 • 535 citations

The Regulatory Role of Macrophages in Antigenic Stimulation Part Two: Symbiotic Relationship between Lymphocytes and Macrophages

1981 • 518 citations

Incorporation of 3H-uridine and 3H-uracil into RNA

1974 • 485 citations

Stress-Induced Modulation of the Immune Response

1977 • 449 citations

Immune responses in vitro. I. Culture conditions for antibody synthesis

1972 • 426 citations

PRIMARY IMMUNE RESPONSE IN CULTURES OF SPLEEN CELLS

1967 • 397 citations

Ouabain-resistant mutants of mouse and hamster cells in culture

1974 • 393 citations

EB virus-induced B lymphocyte cell lines producing specific antibody

1977 • 390 citations

A Method of Increased Sensitivity for detecting Single Antibody-forming Cells

1965 • 381 citations

Activation of Suppressor T Cells during Epstein-Barr-Virus-Induced Infectious Mononucleosis

1979 • 332 citations

Clonal growth and maturation to immunoglobulin secretion in vitro of every growth-inducible B lymphocyte

1977 • 309 citations

Production of human hybridomas secreting antibodies to measles virus

1980 • 308 citations

Influence of Brain and Behavior on the Immune System

1976 • 291 citations

Two genes in the major histocompatibility complex control immune response

1975 • 291 citations

Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200.

1978 • 282 citations

INHIBITORY AND STIMULATORY EFFECTS OF CONCANAVALIN A ON THE RESPONSE OF MOUSE SPLEEN CELL SUSPENSIONS TO ANTIGEN

1972 • 275 citations

CELL INTERACTIONS IN THE IMMUNE RESPONSE IN VITRO

1972 • 253 citations

The Role of Antigen-Specific T Cell Factors in the Immune Response

1980 • 250 citations

Cyclosporin A promotes spontaneous outgrowth in vitro of Epstein–Barr virus-induced B-cell lines

1981 • 242 citations

THE FAILURE OF THYMUS-DERIVED CELLS TO PRODUCE ANTIBODY

1967 • 233 citations

ACTIVATION OF T AND B LYMPHOCYTES IN VITRO

1974 • 231 citations

Immunization of Normal Mouse Spleen Cell Suspensions in vitro

1966 • 230 citations

MECHANISM OF THYMUS-INDEPENDENT IMMUNOCYTE TRIGGERING

1974 • 230 citations

The modulation of lymphocyte functions by molecules secreted by macrophages. I. Description and partial biochemical analysis.

1975 • 229 citations

CARRIER FUNCTION IN ANTI-HAPTEN ANTIBODY RESPONSES

1971 • 225 citations

Thymus-Independent Antigens: The Preparation of Covalent, Hapten-Ficoll Conjugates

1975 • 225 citations

Revised Nomenclature for Antigen-Nonspecific T Cell Proliferation and Helper Factors

1979 • 223 citations

Mouse Mammary Tumors: Alteration of Incidence as Apparent Function of Stress

1975 • 217 citations

Dimethyl sulfoxide enhances polyethylene glycol-mediated somatic cell fusion

1976 • 199 citations

Activation of human B lymphocytes. I. Direct plaque-forming cell assay for the measurement of polyclonal activation and antigenic stimulation of human B lymphocytes.

1976 • 195 citations

Monoclonal Antibodies

1980 • 189 citations

Mycoplasma contamination of animal cell cultures: A simple, rapid detection method

1972 • 188 citations

Monoclonal Antibodies

1981 • 181 citations

A New Approach to the Study of Human B Lymphocyte Function Using an Indirect Plaque Assay and a Direct B Cell Activator

1979 • 181 citations

Fusion of Two Immunoglobulin-producing Myeloma Cells

1973 • 180 citations

An in Vitro Primary Immune Response to 2, 4, 6-Trinitrophenyl Substituted Erythrocytes: Response against Carrier and Hapten

1970 • 178 citations

Identification of a B-cell surface structure involved in antigen-dependent triggering: absence of this structure on B cells from CBA/N mutant mice.

1977 • 178 citations

Stimulation of a T-Independent Primary Anti-Hapten Response in Vitro by TNP-Lipopolysaccharide (TNP-LPS)

1975 • 170 citations

CELL INTERACTIONS IN THE PRIMARY IMMUNE RESPONSE IN VITRO: A REQUIREMENT FOR SPECIFIC CELL CLUSTERS

1969 • 165 citations

Mycoplasmas and cell cultures

1971 • 162 citations

CELL INTERACTIONS IN THE IMMUNE RESPONSE IN VITRO

1972 • 162 citations

B cell helper factors. I. Requirement for both interleukin 2 and another 40,000 mol wt factor.

1981 • 159 citations

Bound C3 as the second signal for B-cell activation

1973 • 158 citations

THE ROLE OF NONLYMPHOID ACCESSORY CELLS IN THE IMMUNE RESPONSE TO DIFFERENT ANTIGENS

1970 • 158 citations

T cell factor which can replace T cells in vivo

1974 • 156 citations

CELL INTERACTIONS IN THE IMMUNE RESPONSE IN VITRO

1972 • 153 citations

Requirements for the establishment of high-titered human monoclonal antibodies against tetanus toxoid using the Epstein-Barr virus technique.

1981 • 153 citations

Human endothelial culture supernatant (HECS): a growth factor for hybridomas.

1980 • 149 citations

Somatic cell hybridization of mouse myeloma cells

1976 • 146 citations

A Selective Medium for Pleuropneumonia-like Organisms

1947 • 144 citations

Lymphocyte reactivity in vitro

1970 • 142 citations

ACTIVATION OF T AND B LYMPHOCYTES IN VITRO

1974 • 142 citations

DNP-Lys-Ficoll: A T-Independent Antigen Which Elicits Both IgM and IgG Anti-DNP Antibody-Secreting Cells

1975 • 138 citations

Comparison of the Ultrastructure of Several Rickettsiae, Ornithosis Virus, andMycoplasmain Tissue Culture

1965 • 137 citations

Regulation of B-lymphocyte clonal proliferation by stimulatory and inhibitory macrophage-derived factors

1977 • 136 citations

Formation of B Lymphocytes in Fetal and Adult Life

1981 • 135 citations

Mycoplasma Infection of Cell Cultures

1978 • 135 citations

CELLULAR REQUIREMENTS FOR THE PRIMARY IN VITRO ANTIBODY RESPONSE TO DNP-FICOLL

1974 • 134 citations

The requirement for macrophages in the in vitro immune response

1971 • 134 citations

THE USE OF BACTERIAL LIPOPOLYSACCHARIDES TO SHOW THAT TWO SIGNALS ARE REQUIRED FOR THE INDUCTION OF ANTIBODY SYNTHESIS

1973 • 133 citations

Specific Collaboration between T and B Lymphocytes across a Cell Impermeable Membrane in vitro

1972 • 132 citations

ANTIGEN-SPECIFIC THYMUS CELL FACTORS IN THE GENETIC CONTROL OF THE IMMUNE RESPONSE TO POLY-(TYROSYL, GLUTAMYL)-POLY-D, L-ALANYL--POLY-LYSYL

1974 • 131 citations

In vitro antigen-induced, antigen-specific antibody production in man. Specific and polyclonal components, kinetics, and cellular requirements.

1981 • 130 citations

Effect of Ethoxyquin on the Longevity of C3H Mice

1971 • 129 citations

Immunoassays in the clinical laboratory

1980 • 129 citations

Induction of polyclonal antibody synthesis by human allogeneic and autologous helper factors.

1979 • 126 citations

Characterization of self-reactive B cells by polyclonal B-cell activators.

1977 • 125 citations

Biochemical Relationship of Thymocyte Mitogenic Factor and Factors Enhancing Humoral and Cell-Mediated Immune Responses

1978 • 125 citations

Frequencies of mitogen-reactive B cells in the mouse. II. Frequencies of B cells producing antibodies which lyse sheep or horse erythrocytes, and trinitrophenylated or nitrodophenylated sheep erythrocytes

1977 • 125 citations

IMMUNE RESPONSES IN VITRO

1971 • 119 citations

B and T cells in Immune Recognition

1977 • 119 citations

Activation of human B lymphocytes. IV. Regulatory effects of corticosteroids on the triggering signal in the plaque-forming cell response of human peripheral blood B lymphocytes to polyclonal activation.

1977 • 117 citations

The modulation of lymphocyte functions by molecules secreted by macrophages. II. Conditions leading to increased secretion.

1976 • 117 citations

Preferential induction of autoantibody secretion in polyclonal activation by peptidoglycan and lipopolysaccharide. II. In vivo studies.

1982 • 115 citations

Production of Antibody to Tetanus Toxoid by Continuous Human Lymphoblastoid Cell Lines

1978 • 115 citations

Interferon as a mediator of human lymphocyte suppression.

1980 • 113 citations

MECHANISM OF ACTIVATION OF THE BONE MARROW-DERIVED LYMPHOCYTE

1973 • 111 citations

Emergence of Suppressor Cells of Immunoglobulin Synthesis During Acute Epstein-Barr Virus-Induced Infectious Mononucleosis

1979 • 111 citations

Human × Mouse Somatic Cell Hybrid Clone secreting Immunoglobulins of both Parental Types

1973 • 110 citations

Nonspecific activation of murine lymphocytes. I. Proliferation and polyclonal activation induced by 2-mercaptoethanol and alpha-thioglycerol.

1977 • 110 citations

Soluble suppressor supernatants elaborated by concanavalin A-activated human mononuclear cells. I. Characterization of a soluble suppressor T cell proliferation.

1981 • 107 citations

Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. III. Dual gene control of the T-cell-mediated suppression of the antibody response.

1976 • 107 citations

Lymphocyte Interactions in Antibody Responses

1972 • 105 citations

Induction of Specific Suppressor T Cells in Vitro

1976 • 105 citations

BIOLOGICAL EXPRESSIONS OF LYMPHOCYTE ACTIVATION

1973 • 104 citations

THE ABROGATION OF SHEEP ERYTHROCYTE TOLERANCE IN RATS BY MEANS OF THE TRANSFER OF ALLOGENEIC LYMPHOCYTES

1970 • 104 citations

Generation of Human Plaque-Forming Cells in Culture: Tissue Distribution, Antigenic and Cellular Requirements

1977 • 100 citations

Continuous production of monoclonal rheumatoid factor by EBV-transformed lymphocytes

1980 • 100 citations

Initiation of Antibody Production to Sheep Erythrocytes in Vitro: Replacement of the Requirement for T-Cells with a Cell-Free Factor Isolated from Cultures of Lymphoid Cells

1972 • 99 citations

In vitro immunization as an adjunct to the production of hybridomas producing antibodies against the lymphokine osteoclast activating factor

1980 • 98 citations

Suppressive Effect of Interferon on the Humoral Immune Response to Sheep Red Blood Cells in Mice

1974 • 95 citations

Antigen-specific and nonspecific mediators of T cell/B cell cooperation. I. Evidence for their production by different T cells.

1975 • 94 citations

Methods for derivation and detection of anti-parasite monoclonal antibodies

1980 • 94 citations

Enhancement of the Humoral Response of T Cell-Depleted Murine Spleens by a Factor Derived from Human Monocytes in Vitro

1974 • 94 citations

An autoradiographic screening test for mycoplasmal contamination of mammalian cell cultures

1973 • 92 citations

The requirement of an adherent cell substratum for the growth of developing plasmacytoma cells in vivo.

1976 • 90 citations

The isolation of heterokaryons and hybrids by a selective system using irreversible biochemical inhibitors

1978 • 90 citations

Function of 2-Mercaptoethanol as a Macrophage Substitute in the Primary Immune Response in Vitro

1976 • 90 citations

Use of cloned populations of mouse lymphocytes to analyze cellular differentiation

1981 • 88 citations

Rapid detection of mycoplasma-infected cell cultures*

1971 • 88 citations

The role of humoral factors in the initiation of in vitro primary immune responses. II. Effects of lymphocyte mitogens.

1973 • 88 citations

THE ALLOGENEIC EFFECT IN INBRED MICE

1972 • 88 citations

Lipopolysaccharides can convert heterologous red cells into thymus-independent antigens

1972 • 87 citations

Molecular and Quantitative Analysis of Helper T Cell-Replacing Factors on the Induction of Antigen-Sensitive B and T Lymphocytes

1979 • 86 citations

Preferential retention of human chromosome 14 in mouse × human B cell hybrids

1980 • 86 citations

Antigen-specific and nonspecific mediators of T cell/B cell cooperation. III. Characterization of the nonspecific mediator(s) from different sources.

1976 • 85 citations

Biochemical separation of a human B cell mitogenic factor.

1982 • 84 citations

INCIDENCE AND DETECTION OF PLEUROPNEUMONIA-LIKE ORGANISMS IN CELL CULTURES BY FLUORESCENT ANTIBODY AND CULTURAL PROCEDURES

1962 • 84 citations

In Vitro Studies of Immunological Responses of Lymphoid Cells

1967 • 83 citations

INDUCTION OF ANTIBODY FORMATION AND TOLERANCE IN VITRO TO A PURIFIED PROTEIN ANTIGEN

1967 • 83 citations

The Purification and Quantitation of Helper T Cell-Replacing Factors Secreted by Murine Spleen Cells Activated by Concanavalin A

1979 • 82 citations

PRIMARY ANTIBODY RESPONSE IN ORGAN CULTURES

1966 • 81 citations

Immunocytology of cultured IgM-forming cells of mouse. I. Requirement of phagocytic cell factor for the growth of IgM-forming tumor cells in tissue culture.

1972 • 81 citations

THE REQUIREMENT FOR HYDROCORTISONE IN ANTIBODY-FORMING TISSUE CULTIVATED IN SERUM-FREE MEDIUM

1964 • 81 citations

Evidence for the identification of lymphocyte activating factor as the adherent cell-derived mediator responsible for enhanced antibody synthesis by nude mouse spleen cells

1978 • 80 citations

Long-term culture and cloning of nontransformed human B lymphocytes.

1981 • 78 citations

THE IDENTIFICATION AND SOURCES OF MYCOPLASMAS ISOLATED FROM CONTAMINATED CELL CULTURES

1973 • 77 citations

Multiple Pathways of B Lymphocyte Tolerance

1979 • 76 citations

The Antibody Repertoire of Hybrid Cell Lines Obtained by Fusion of X63-AG8 Myeloma Cells with Mitogen-Activated B-Cell Blasts

1979 • 74 citations

Cell components in the immune response

1970 • 73 citations

Direct triggering of B lymphocytes by insolubilized antigen

1974 • 72 citations

Characteristics of Epstein-Barr virus activation of human B lymphocytes.

1981 • 72 citations

In vitro studies on H‐2‐linked unresponsiveness to synthetic polypeptides

1977 • 72 citations

Human Lymphoblastoid Cell Line Producing Specific Antibody against Rh‐Antigen D

1980 • 71 citations

Cellular interactions affecting the maturation of murine B lymphocyte precursors in vitro.

1981 • 71 citations

Microscopic demonstration of mycoplasma contamination in cell cultures and cell culture media

1976 • 70 citations

PRIMARY IN VITRO ANTIBODY RESPONSE FROM HUMAN PERIPHERAL BLOOD LYMPHOCYTES

1977 • 70 citations

Immune Response in vitro: Independence of "Activated" Lymphoid Cells

1969 • 70 citations

THE PRODUCTION OF ANTIBODIES BY TISSUES LIVING OUTSIDE OF THE ORGANISM

1912 • 70 citations

Cell components in the immune response

1970 • 69 citations

Detection of mycoplasma in cell cultures

1967 • 68 citations

Spread and control of mycoplasmal infection of cell cultures

1976 • 68 citations

The role of fetal calf serum in the primary immune response in vitro.

1977 • 67 citations

The specificity of T-cell helper factor in man

1977 • 67 citations

Soluble suppressor supernatants elaborated by concanavalin A-activated human mononuclear cells. II. Characterization of a soluble suppressor of B cell immunoglobulin production.

1981 • 67 citations

Establishment of Anti‐TNP Antibody‐producing Human Lymphoid Lines by Preselection for Hapten Binding followed by EBV Transformation

1979 • 67 citations

Human B Cell Function in a Polyclonally Induced Plaque Forming Cell System. Cell Triggering and Immunoregulation

1979 • 66 citations

Induction of antigen-specific human suppressor T lymphocytes in vitro

1978 • 66 citations

The necessity for T cell help for human tonsil B cell responses to pokeweed mitogens: induction of DNA synthesis, immunoglobulin, and specific antibody production with a T cell helper factor produced with pokeweed mitogen.

1977 • 65 citations

Characterization of an Immunosuppressive Factor Present in Mouse Serum

1973 • 64 citations

In vitro Reconstitution of the Immune Response of Thymus-deprived Mice to Sheep Red Blood Cells

1970 • 64 citations

Mechanism of action of human B cell-activating factor. I. Comparison of the plaque-stimulating activity with thymocyte-stimulating activity.

1979 • 63 citations

Restoration of Antibody-Forming Capacity in Cultures of Nonadherent Spleen Cells by Mercaptoethanol

1972 • 62 citations

Modulation of B Cell Responses by Glycolipid Released from Antigen-Stimulated T Cells

1977 • 61 citations

Production of Human IgM Anti-D in Tissue Culture by EB-Virus-transformed Lymphocytes

1980 • 61 citations

T cell-mediated immunoregulation of Epstein Barr virus- (EBV) induced B lymphocyte activation in EBV-seropositive and EBV-seronegative individuals.

1982 • 61 citations

The Role of Humoral Factors in the Initiation of in Vitro Primary Immune Responses

1973 • 60 citations

Induction of Immunity and Tolerance to the Dinitrophenyl Determinant in vitro

1971 • 60 citations

Preferential induction of autoantibody secretion in polyclonal activation by peptidoglycan and lipopolysaccharide. I. In vitro studies.

1982 • 60 citations

In vitro induction and measurement of hemolytic plaque forming cells in man

1976 • 59 citations

Purification and Properties of Human B Cell-Activating Factor

1979 • 59 citations

Alloreactive cloned T cell lines. V. Differential kinetics of IL 2, CSF, and BCSF release by a cloned T amplifier cell and its variant.

1981 • 58 citations

Production of Antibody to Sheep Red Blood Cells by Human Tonsil Cells in vitro

1973 • 57 citations

Primary in vitro antibody responses by purified murine B lymphocytes in serum-free defined medium.

1981 • 56 citations

Reconstitution of t cell-depleted spleen cell populations by factors derived from t cells. I. Conditions for the production of active t cell supernatants.

1973 • 56 citations

THE THYMUS YESTERDAY, TODAY, AND TOMORROW

1967 • 55 citations

Induction of an antibody response in cultures of human peripheral blood lymphocytes.

1976 • 55 citations

Regulation of in vitro primary anti-DNP antibody production by functional subsets of T lymphocytes in man.

1981 • 55 citations

Antigen-Specific and Nonspecific Mediators of T Cell/B Cell Cooperation

1975 • 55 citations

Resolution of a factor that enhances the antibody response of T cell-depleted murine splenocytes from several other monocyte products

1976 • 55 citations

Sera and the in vitro induction of immune responses. I. Bacterial contamination and the generation of good fetal bovine sera.

1975 • 55 citations

Mitogen-activated B-cell blasts reactive to more than one mitogen.

1979 • 53 citations

Fusion of in vitro Immunized Lymphoid Cells with X63Ag8

1979 • 53 citations

Polyclonal Activation of Human B Lymphocytes by Nocardia Water Soluble Mitogen (NWSM)

1979 • 52 citations

Activation of Human B Lymphocytes

1977 • 52 citations

Antibiotic Control of Mycoplasma in Tissue Culture

1967 • 51 citations

Depression of macrophages in mice drinking hyperchlorinated water

1977 • 51 citations

T‐CELL REGULATION OF IMMUNE RESPONSIVENESS*

1975 • 50 citations

Antigen-specific suppressor T cell factors

1980 • 50 citations

Induction of plaque-forming cells in cultured human lymphocytes by combined action of antigen and EB virus

1977 • 50 citations

Polyclonal activation of human B lymphocytes by Fc fragments. I. Characterization of the cellular requirements for Fc fragment-mediated polyclonal antibody secretion by human peripheral blood B lymphocytes.

1981 • 49 citations

Specific anti-influenza virus antibody production in vitro by human peripheral blood mononuclear cells.

1981 • 49 citations

Primary in Vitro Antibody Response from Human Peripheral Blood Lymphocytes

1977 • 49 citations

Antibody Formation in Nonimmune Mouse Peritoneal Cells after Incubation in Gum Containing Antigen

1966 • 48 citations

The Role of Receptor IgM and IgD in Determining Triggering and Induction of Tolerance in Murine B Cells¹

1979 • 48 citations

Isolation of Mycoplasma From Leukemic and Nonleukemic Patients<xref ref-type="fn" rid="FN2">2</xref>

1970 • 48 citations

Mechanism of Action of Human B Cell-Activating Factor

1979 • 48 citations

Isolation of <italic>Mycoplasma orale</italic> From Leukemic Bone Marrow and Blood by Direct Culture

1966 • 47 citations

Stimulation of the release of a B cell-activating factor from human monocytes

1976 • 47 citations

Antigen-Specific and Nonspecific Mediators of T Cell/B Cell Cooperation

1976 • 47 citations

Induction of Antibody Synthesis in Human B Lymphocytes by Different Polyclonal B Cell Activators: Evaluation by Direct and Indirect PFC Assays

1979 • 47 citations

Immunocytology of Cultured IgM-Forming Cells of Mouse

1972 • 46 citations

The in vitro response to sheep erythrocytes by mouse spleen cells: Segregation of distinct events leading to antibody formation

1974 • 45 citations

Mycoplasma contamination of cell cultures *1Interference with 32P-labelling pattern of RNA

1969 • 45 citations

Reconstitution of T Cell-Depleted Spleen Cell Populations by Factors Derived from T Cells

1973 • 44 citations

Regulation of Human B Cell Activation

1979 • 44 citations

Serological analysis of antigen‐specific helper factors specific for poly‐<scp>L</scp>(Tyr, Glu)‐poly‐<scp>DL</scp>Ala–poly‐<scp>L</scp>Lys [(T, G)‐A–L] and <scp>L</scp>Glu⁶⁰‐<scp>L</scp>Ala³⁰‐<scp>L</scp>Tyr¹⁰ (GAT)

1979 • 44 citations

In vitro generation of antigen-specific hemolytic plaque-forming cells from human peripheral blood mononuclear cells.

1981 • 44 citations

A Micro-Technique for Hemolytic Plaque Assays

1974 • 43 citations

T-cell regulation of human peripheral blood B-cells responsiveness.

1977 • 42 citations

Detection of mycoplasma contamination in cultured human fibroblasts

1973 • 41 citations

Human endothelial culture supernatant (HECS): evidence for a growth-promoting factor binding to hybridoma and myeloma cells.

1981 • 41 citations

Induction of Human Antibody Responses In Vitro with Emphasis on Allogeneic Helper Factors

1979 • 40 citations

INDUCTION OF ANTIBODIES IN VITRO

1969 • 40 citations

Effect of PPD on the Specific Immune Response to Heterologous Red Cells in Vitro

1974 • 39 citations

Role of accessory cells in B cell activation. IV. Ia+ accessory cells are required for the in vitro generation of thymic independent type 2 antibody responses to polysaccharide antigens.

1981 • 39 citations

IgG response in vitro. I. The requirement for an intermediate responsive cell type

1976 • 39 citations

The Necessity for T Cell Help for Human Tonsil B Cell Responses to Pokeweed Mitogen: Induction of DNA Synthesis, Immunoglobulin, and Specific Antibody Production with a T Cell Helper Factor Produced with Pokeweed Mitogen

1977 • 39 citations

Procedures to reduce contamination of cell cultures

1971 • 39 citations

The Serum Factor Stimulating Colony Formation In Vitro by Murine Plasmacytoma Cells: Response to Antigens and Mineral Oil

1974 • 38 citations

Immunoglobulin production by a human-mouse somatic cell hybrid

1975 • 37 citations

Growth of human B-lymphocyte colonies in vitro

1979 • 37 citations

Immune response (Ir) genes expressed at macrophage–B lymphocyte interactions

1978 • 34 citations

Triggering of Human Peripheral Blood B Cells: Polyclonal Induction and Modulation of an in Vitro PFC Response

1976 • 34 citations

Cellular and Molecular Restrictions of the Lymphocyte Fusion

1979 • 34 citations

Establishment and characterization of continuous helper T cell lines specific to poly(LTyr,LGlu)-poly(DLAla)--poly(LLys).

1981 • 34 citations

Elimination of mycoplasmas from cell cultures with sodium polyanethol sulphonate

1975 • 33 citations

in vitro studies of allotype suppression in mice

1973 • 33 citations

Antibiotic Control of Mycoplasma in Tissue Culture

1967 • 33 citations

Identification and Partial Purification of Two Synergistically Acting Helper Mediators in Human Mixed Leukocyte Culture Supernatants

1977 • 32 citations

Lymphocyte Subpopulations in Man: Suppression of PWM‐induced B‐Cell Proliferation by Infectious Mononucleosis T Cells

1979 • 32 citations

In vitro immune response of human peripheral lymphocytes. V. PHA- and protein A-induced human B colony formation and analysis of the subpopulations of B cells.

1980 • 32 citations

Continuously proliferating allospecific T cells. I. Specificity of cooperation with allogeneic B cells in the humoral antibody response to sheep erythrocytes.

1979 • 31 citations

Glucose and glutamate metabolism ofLegionella pneumophila

1980 • 31 citations

H-2-restricted helper factor secreted by clone hybridoma cells.

1981 • 31 citations

Specific In Vitro IgM Responses of Human B Cells: A Complex Regulatory Network Modulated by Antigen

1979 • 30 citations

Human B lymphocyte colony responses. I. General characteristics and modulation by monocytes.

1980 • 30 citations

Antibody Synthesis Initiated in vitro by Paired Explants of Spleen and Thymus

1966 • 29 citations

Sera and the in Vitro Induction of Immune Responses

1975 • 29 citations

Nonspecific “Lymphocyte Activating” Factors Produced by Macrophages

1979 • 28 citations

Studies on the Mechanism of Stimulation of the Humoral Response of Murine Spleen Cultures by Culture Fluids from Human Monocytes

1975 • 28 citations

Establishment of Specific Antibody Producing Human Lines by Antigen Preselection and EBV-Transformation

1979 • 27 citations

Clones of Human Lymphoblastoid Cell Lines Producing Antibody to Tetanus Toxoid

1979 • 27 citations

Reconstitution of immunocompetence in B cells by addition of concanavalin A or concanavalin A-treated thymus cells.

1973 • 27 citations

Human lymphocytes can generate thymus-independent as well as thymus-dependent anti-hapten plaque-forming cell responses in vitro.

1981 • 26 citations

In Vitro Antibody Response to Trinitrophenyl‐Polyacrylamide Beads

1979 • 26 citations

Modulation of the human in vitro antibody response by human leukocyte interferon preparations

1981 • 26 citations

Activation of human B lymphocytes. IX. Modulation of antibody production by products of activated macrophages.

1978 • 26 citations

Molecular Identification of a Surface Structure on B Cells (Lyb-3) and Its Relationship to B Cell Triggering

1978 • 25 citations

Suppressor cells in rabbit peripheral blood

1976 • 25 citations

Inhibition of secondary in vitro antibody responses by the third component of complement.

1982 • 24 citations

T cell replacing factor substitutes for an I-J+ idiotype-specific T helper cell.

1982 • 24 citations

Characterization of a soluble suppressor of human B cell immunoglobulin biosynthesis produced by a continuous human suppressor T cell line.

1981 • 24 citations

Antibody Production in Man: In Vitro Synthesis and Clinical Implications

1980 • 23 citations

Kinetics of in vitro initiated secondary anti-hapten response: induction of plaque-forming cells by soluble and particulate antigen

1970 • 23 citations

Properties of educated T cells. The ability of educated T cells to facilitate the immune response to non‐cross‐reacting antigens in vitro

1973 • 23 citations

Induction of allospecific suppressor T Cells in vitro

1977 • 22 citations

In vitro analysis of allogeneic lymphocyte interaction. II. I-region control of the activity of a B-cell-derived H-2-restricted allogeneic effect factor and its receptor during B-cell activation.

1978 • 22 citations

In vivo and in vitro production and detection of monoclonal antibodies to surface components on metastatic variants of murine tumor cells.

1981 • 22 citations

The xenogeneic effect. I. Antigen and mitogen-stimulated human lymphocytes produce a non-antigen-specific factor which reconstitutes the antibody response of T cell-deficient mouse spleen cells.

1975 • 22 citations

Detection and control of occult mycoplasma contamination in human tumor cell lines

1980 • 22 citations

Flow sorting of antigen-binding B cell subsets.

1980 • 22 citations

A T‐CELL‐PRODUCED MEDIATOR SUBSTANCE ACTIVE IN THE HUMORAL IMMUNE RESPONSE

1975 • 22 citations

Helper activity in vitro to a defined determinant

1974 • 21 citations

ROLE OF MITOGENIC FACTOR, LYMPHOCYTE‐ACTIVATING FACTOR AND IMMUNE INTERFERON IN THE INDUCTION OF HUMORAL AND CELL‐MEDIATED IMMUNITY

1979 • 21 citations

Two separate genes regulate self-Ia and carrier recognition in H-2-restricted helper factors secreted by hybridoma cells.

1981 • 21 citations

The xenogeneic effect. II. Requirement for unactivated murine T cells during restoration of immune responsiveness with xenogeneic reconstitution factor.

1976 • 20 citations

Activation of mouse lymphocytes by anti-immunoglobulin. III. Analysis of responding B lymphocytes by flow cytometry and cell sorting.

1981 • 20 citations

Growth of mouse plasmacytoma cells in serum-free, hormone-supplemented medium: Procedure for the determination of hormone and growth factor requirements for cell growth

1981 • 20 citations

Immunogenicity In Vitro: Structural Correlation

1974 • 20 citations

A simple biochemical method for the detection of mycoplasmas and other microbial contaminants of cell cultures

1976 • 20 citations

Detection of acceptor sites on human lymphocytes for antigen-specific T cell factors

1977 • 19 citations

Hapten-specific murine colony-forming B cells: in vitro response of colonies to fluoresceinated thymus independent antigens.

1981 • 19 citations

The role of humoral factors in the initiation of in vitro primary immune responses. I. Effects of deficient fetal bovine serum.

1973 • 19 citations

Antibiotic resistance in Mycoplasma isolates from tissue cultures

1967 • 19 citations

Immunocytology of cultured IgM-forming cells of mouse

1974 • 19 citations

Conditions for the development of IgM- and IgG-antibody-secreting cells from primed mouse splenocytes in vitro.

1977 • 17 citations

In vitro analysis of allogeneic lymphocyte interaction. III. Generation of a helper allogeneic effect factor (AEF) across an I-J subregion disparity.

1979 • 17 citations

Monoclonal antibodies. Hybrid myelomas--a revolution in serology and immunogenetics.

1979 • 17 citations

Induction of plaque-forming cells in human blood lymphocytes cultured in the presence of antigen and Epstein-Barr virus: a study with normal donors and infectious mononucleosis patients.

1979 • 17 citations

The Xenogeneic Effect

1976 • 16 citations

Mucigenic and Adjuvant Activity of a Methanol Extraction Residue (HER) of Tubercle Bacilli on Mouse Lymphoid Cells in Vitro

1975 • 16 citations

The ability of nonspecific T-cell stimulators to induce helper-cell-dependent increases in either polyclonal or isotype-restricted Ig production in vivo

1981 • 16 citations

The Use of Allogeneic T Lymphocytes and Bacterial Lipopolysaccharide to Induce Immune Responses to Monovalent Haptens in Vitro

1974 • 16 citations

Activation of Human B Lymphocytes

1978 • 16 citations

MACROPHAGE FACTORS THAT ENHANCE THE ANTIBODY RESPONSE*

1979 • 16 citations

B and T lymphocyte colony formation in agar: 2-Mercaptoethanol-activated albumin can substitute for 2-mercaptoethanol

1979 • 15 citations

In vitro immune response of human peripheral lymphocytes. VI. Distribution and characterization of precursors for PHA- and protein A-induced colony-forming B cells.

1980 • 15 citations

Hybridomas and Cellular Immortality

1983 • 15 citations

T cell-dependent mediator and B-cell clones.

1975 • 14 citations

The Role of Humoral Factors in the Initiation of in Vitro Primary Immune Responses

1973 • 14 citations

Carrageenan stimulates populations of mouse “B” cells mostly nonoverlapping with those stimulated with LPS or dextran sulfate

1981 • 14 citations

CONTROL OF B‐CELL DIFFERENTIATION BY MACROPHAGES*

1979 • 14 citations

The effects of various mammalian sera on attachment efficiency and thymidine incorporation in primary cultures of mouse mammary epithelial cells

1977 • 14 citations

Inhibition of 3H-thymidine incorporation by hydroxyurea: Atypical response of mycoplasma-infected cells in culture

1975 • 14 citations

A serum‐free medium for the Mishell‐Dutton system

1977 • 13 citations

The Xenogeneic Effect

1975 • 13 citations

FACTORS INFLUENCING ACTIVATION OF B‐CELLS IN IMMUNITY*

1975 • 13 citations

Antibiotic resistance in Mycoplasma isolates from tissue cultures.

1967 • 13 citations

Antigen-specific helper factor reacts with antibodies to human beta 2 microglobulin.

1981 • 12 citations

Human splenic and peripheral blood lymphocyte response to lipopolysaccharide

1981 • 12 citations

In vitro anti-hapten response to a lactoside-conjugated protein

1975 • 12 citations

Mouse-Human Hybridomas. The Conversion of Non-Secreting Human B Cells into Ig Secretors

1979 • 11 citations

Human lymphocyte-mouse myeloma somatic cell hybrids: Selective hybrid formation

1977 • 11 citations

Irreversible Receptor Modulation on B Lymphocytes and the Control of Antibody‐Forming Cells by Antigen

1979 • 11 citations

In vitro immune response of human peripheral lymphocytes. VII. Effect of anti-mu and anti-delta antibodies on B colony formation and detection of abnormal B cells in patients with juvenile rheumatoid arthritis.

1980 • 11 citations

Cellular Cooperation and Stimulatory Factors in Antibody Responses: Limiting Dilution Analysis in Vitro

1974 • 10 citations

Partial characterization of an immunoenhancing factor from allogeneic human lymphocyte cell lines.

1976 • 10 citations

Sera and the in vitro induction of immune responses. II. Inhibitory effects of newborn calf sera on primary humoral responses.

1975 • 10 citations

Preliminary chemical and biologic characterization of (Fc)TRF: an Fc fragment-induced T cell-replacing factor.

1982 • 10 citations

Localization of Calmodulin in Tissue Culture Cells

1982 • 9 citations

Production of Specific Antibody Without Specific Immunization

1979 • 9 citations

Concanavalin A supernatant recruits antigen-insensitive IgG memory B lymphocyte precursors into an antigen-sensitive precursor pool.

1982 • 9 citations

COMPARISON OF THE PLAQUE‐STIMULATING AND THYMOCYTE‐STIMULATING ACTIVITIES DERIVED FROM HUMAN MONOCYTES

1979 • 9 citations

“SIGNAL THEORY” IN CELLULAR IMMUNOLOGY: COLLABORATION BETWEEN T‐ AND B‐LYMPHOCYTES IN THE IMMUNE RESPONSE

1975 • 9 citations

B-memory cells can be stimulated by antigen in vitro to become IgG antibody-secreting cells.

1977 • 8 citations

In Vitro Analysis of Allogeneic Lymphocyte Interaction

1979 • 8 citations

Macrophage Requirement for the in Vitro Response to an Insolubilized T‐independent Antigen

1979 • 7 citations

Anti-immunoglobulin stimulation of murine lymphocytes. VII. Identification of an age-dependent change in accessory cell function.

1982 • 7 citations

Hapten-specific murine colony-forming B cells. II. Delineation of a tolerogen-sensitive subpopulation of colony-forming B cells.

1981 • 7 citations

Early history of specific antibody-producing lymphocyte hybridomas.

1981 • 6 citations

B Lymphocyte Colony Formation in vitro: Ultrastructural Development of Individual Colonies

1980 • 6 citations

Induction of IgM Secretion by Fusing Murine B-Lymphoma with Myeloma Cells

1979 • 6 citations

Activation of human B lymphocytes

1981 • 5 citations

Sera and the in Vitro Induction of Immune Responses

1975 • 5 citations

Formation of heterophile antibodies by human tonsilar lymphocytes

1981 • 5 citations

In vitro Approach to Development of Immune Reactivity

1976 • 5 citations

Effects of the MER tubercle bacillus fraction on the production of antibodies in vitro

1980 • 5 citations

Letters to the Editor

1976 • 4 citations

The plaque-forming-cell (PFC) response of human blood lymphocytes

1981 • 4 citations

Specific helper factors

1980 • 4 citations

STIMULATION OF B‐IMMUNOCYTES BY A T‐CELL FACTOR PRODUCED IN MIXED LEUKOCYTE CULTURES*

1975 • 4 citations

B-cell subsets responsive to fluorescein-conjugated antigens

1981 • 3 citations

Anti-immunoglobulin stimulation of murine lymphocytes

1981 • 2 citations

Purification and characterization of a mouse T cell replacing factor from primary mixed lymphocyte culture supernatant

1980 • 2 citations